Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/rda.14077 http://hdl.handle.net/11449/223243 |
Resumo: | The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n = 21) or 12-day (G-12SOV, n = 21) progesterone (P4)-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6–7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p <.05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p >.05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p <.05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p >.05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4-device removal) was positively correlated (p <.05) with the number of CL (r =.95) and viable embryos (r =.91) in G-12SOV. The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes. |
id |
UNSP_a3cf32bc28c029ed9eca1b395c1d7e65 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/223243 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushingin vivo embryo productionnaturalized sheep breednon-surgical embryo recoveryNSERsuperovulationThe present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n = 21) or 12-day (G-12SOV, n = 21) progesterone (P4)-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6–7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p <.05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p >.05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p <.05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p >.05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4-device removal) was positively correlated (p <.05) with the number of CL (r =.95) and viable embryos (r =.91) in G-12SOV. The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual PaulistaEmbrapa Caprinos e OvinosDepartamento de Reprodução e Avaliação Animal Universidade Federal Rural do Rio de JaneiroFaculdade de Veterinária Universidade Federal FluminenseEmbrapa Pecuária SudesteDepartment of Biomedical Sciences Ontario Veterinary College University of GuelphFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual PaulistaFAPEMIG: CVZ-PPM 00201-17Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal Rural do Rio de JaneiroUniversidade Federal Fluminense (UFF)University of GuelphOliveira, Maria Emilia Franco [UNESP]Arrais, Aline Matosde Mello, Marco Roberto BourgVergani, Gabriel Brun [UNESP]Figueira, Lucas MachadoEsteves, Sérgio NovitaPereira, Verônica Schinaider do AmaralGarcia, Alexandre RossettoBartlewski, Pawel Mieczyslawda Fonseca, Jeferson Ferreira2022-04-28T19:49:32Z2022-04-28T19:49:32Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article393-401http://dx.doi.org/10.1111/rda.14077Reproduction in Domestic Animals, v. 57, n. 4, p. 393-401, 2022.1439-05310936-6768http://hdl.handle.net/11449/22324310.1111/rda.140772-s2.0-85122747293Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccess2022-04-28T19:49:32Zoai:repositorio.unesp.br:11449/223243Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:49:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
title |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
spellingShingle |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing Oliveira, Maria Emilia Franco [UNESP] in vivo embryo production naturalized sheep breed non-surgical embryo recovery NSER superovulation |
title_short |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
title_full |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
title_fullStr |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
title_full_unstemmed |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
title_sort |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing |
author |
Oliveira, Maria Emilia Franco [UNESP] |
author_facet |
Oliveira, Maria Emilia Franco [UNESP] Arrais, Aline Matos de Mello, Marco Roberto Bourg Vergani, Gabriel Brun [UNESP] Figueira, Lucas Machado Esteves, Sérgio Novita Pereira, Verônica Schinaider do Amaral Garcia, Alexandre Rossetto Bartlewski, Pawel Mieczyslaw da Fonseca, Jeferson Ferreira |
author_role |
author |
author2 |
Arrais, Aline Matos de Mello, Marco Roberto Bourg Vergani, Gabriel Brun [UNESP] Figueira, Lucas Machado Esteves, Sérgio Novita Pereira, Verônica Schinaider do Amaral Garcia, Alexandre Rossetto Bartlewski, Pawel Mieczyslaw da Fonseca, Jeferson Ferreira |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Federal Rural do Rio de Janeiro Universidade Federal Fluminense (UFF) University of Guelph |
dc.contributor.author.fl_str_mv |
Oliveira, Maria Emilia Franco [UNESP] Arrais, Aline Matos de Mello, Marco Roberto Bourg Vergani, Gabriel Brun [UNESP] Figueira, Lucas Machado Esteves, Sérgio Novita Pereira, Verônica Schinaider do Amaral Garcia, Alexandre Rossetto Bartlewski, Pawel Mieczyslaw da Fonseca, Jeferson Ferreira |
dc.subject.por.fl_str_mv |
in vivo embryo production naturalized sheep breed non-surgical embryo recovery NSER superovulation |
topic |
in vivo embryo production naturalized sheep breed non-surgical embryo recovery NSER superovulation |
description |
The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV, n = 21) or 12-day (G-12SOV, n = 21) progesterone (P4)-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6–7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p <.05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p >.05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p <.05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p >.05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4-device removal) was positively correlated (p <.05) with the number of CL (r =.95) and viable embryos (r =.91) in G-12SOV. The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:49:32Z 2022-04-28T19:49:32Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/rda.14077 Reproduction in Domestic Animals, v. 57, n. 4, p. 393-401, 2022. 1439-0531 0936-6768 http://hdl.handle.net/11449/223243 10.1111/rda.14077 2-s2.0-85122747293 |
url |
http://dx.doi.org/10.1111/rda.14077 http://hdl.handle.net/11449/223243 |
identifier_str_mv |
Reproduction in Domestic Animals, v. 57, n. 4, p. 393-401, 2022. 1439-0531 0936-6768 10.1111/rda.14077 2-s2.0-85122747293 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction in Domestic Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
393-401 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965609484615680 |