Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis

Detalhes bibliográficos
Autor(a) principal: Bernardino, Pedro Negri [UNESP]
Data de Publicação: 2023
Outros Autores: de Paula, Carolina Lechinski [UNESP], Pereira, Ana Flávia Marques [UNESP], Ribeiro, Márcio Garcia [UNESP], de Carvalho Azevedo, Vasco Ariston, Borges, Alexandre Secorun [UNESP], Fernandes-Júnior, Ary [UNESP], Oliveira-Filho, José Paes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s42770-022-00885-0
http://hdl.handle.net/11449/249483
Resumo: Caseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.
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spelling Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis2’,3’-DideoxyadenosineBactericidalBacteriostaticCaseous lymphadenitisSmall ruminantCaseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)School of Veterinary Medicine Molecular Biosciences Department University of California – DavisInstitute of Biosciences São Paulo State University (UNESP)Genetics Ecology and Evolution Department Federal University of Minas GeraisSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)CNPq: 132282/2019Universidade Estadual Paulista (UNESP)University of California – DavisUniversidade Federal de Minas Gerais (UFMG)Bernardino, Pedro Negri [UNESP]de Paula, Carolina Lechinski [UNESP]Pereira, Ana Flávia Marques [UNESP]Ribeiro, Márcio Garcia [UNESP]de Carvalho Azevedo, Vasco AristonBorges, Alexandre Secorun [UNESP]Fernandes-Júnior, Ary [UNESP]Oliveira-Filho, José Paes [UNESP]2023-07-29T15:42:32Z2023-07-29T15:42:32Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article559-563http://dx.doi.org/10.1007/s42770-022-00885-0Brazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023.1678-44051517-8382http://hdl.handle.net/11449/24948310.1007/s42770-022-00885-02-s2.0-85144136740Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T15:42:32Zoai:repositorio.unesp.br:11449/249483Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T15:42:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
title Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
spellingShingle Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
Bernardino, Pedro Negri [UNESP]
2’,3’-Dideoxyadenosine
Bactericidal
Bacteriostatic
Caseous lymphadenitis
Small ruminant
title_short Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
title_full Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
title_fullStr Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
title_full_unstemmed Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
title_sort Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
author Bernardino, Pedro Negri [UNESP]
author_facet Bernardino, Pedro Negri [UNESP]
de Paula, Carolina Lechinski [UNESP]
Pereira, Ana Flávia Marques [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Carvalho Azevedo, Vasco Ariston
Borges, Alexandre Secorun [UNESP]
Fernandes-Júnior, Ary [UNESP]
Oliveira-Filho, José Paes [UNESP]
author_role author
author2 de Paula, Carolina Lechinski [UNESP]
Pereira, Ana Flávia Marques [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Carvalho Azevedo, Vasco Ariston
Borges, Alexandre Secorun [UNESP]
Fernandes-Júnior, Ary [UNESP]
Oliveira-Filho, José Paes [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of California – Davis
Universidade Federal de Minas Gerais (UFMG)
dc.contributor.author.fl_str_mv Bernardino, Pedro Negri [UNESP]
de Paula, Carolina Lechinski [UNESP]
Pereira, Ana Flávia Marques [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Carvalho Azevedo, Vasco Ariston
Borges, Alexandre Secorun [UNESP]
Fernandes-Júnior, Ary [UNESP]
Oliveira-Filho, José Paes [UNESP]
dc.subject.por.fl_str_mv 2’,3’-Dideoxyadenosine
Bactericidal
Bacteriostatic
Caseous lymphadenitis
Small ruminant
topic 2’,3’-Dideoxyadenosine
Bactericidal
Bacteriostatic
Caseous lymphadenitis
Small ruminant
description Caseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T15:42:32Z
2023-07-29T15:42:32Z
2023-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s42770-022-00885-0
Brazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023.
1678-4405
1517-8382
http://hdl.handle.net/11449/249483
10.1007/s42770-022-00885-0
2-s2.0-85144136740
url http://dx.doi.org/10.1007/s42770-022-00885-0
http://hdl.handle.net/11449/249483
identifier_str_mv Brazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023.
1678-4405
1517-8382
10.1007/s42770-022-00885-0
2-s2.0-85144136740
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 559-563
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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