Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s42770-022-00885-0 http://hdl.handle.net/11449/249483 |
Resumo: | Caseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections. |
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Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis2’,3’-DideoxyadenosineBactericidalBacteriostaticCaseous lymphadenitisSmall ruminantCaseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)School of Veterinary Medicine Molecular Biosciences Department University of California – DavisInstitute of Biosciences São Paulo State University (UNESP)Genetics Ecology and Evolution Department Federal University of Minas GeraisSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)CNPq: 132282/2019Universidade Estadual Paulista (UNESP)University of California – DavisUniversidade Federal de Minas Gerais (UFMG)Bernardino, Pedro Negri [UNESP]de Paula, Carolina Lechinski [UNESP]Pereira, Ana Flávia Marques [UNESP]Ribeiro, Márcio Garcia [UNESP]de Carvalho Azevedo, Vasco AristonBorges, Alexandre Secorun [UNESP]Fernandes-Júnior, Ary [UNESP]Oliveira-Filho, José Paes [UNESP]2023-07-29T15:42:32Z2023-07-29T15:42:32Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article559-563http://dx.doi.org/10.1007/s42770-022-00885-0Brazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023.1678-44051517-8382http://hdl.handle.net/11449/24948310.1007/s42770-022-00885-02-s2.0-85144136740Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T15:42:32Zoai:repositorio.unesp.br:11449/249483Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T15:42:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
title |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
spellingShingle |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis Bernardino, Pedro Negri [UNESP] 2’,3’-Dideoxyadenosine Bactericidal Bacteriostatic Caseous lymphadenitis Small ruminant |
title_short |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
title_full |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
title_fullStr |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
title_full_unstemmed |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
title_sort |
Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis |
author |
Bernardino, Pedro Negri [UNESP] |
author_facet |
Bernardino, Pedro Negri [UNESP] de Paula, Carolina Lechinski [UNESP] Pereira, Ana Flávia Marques [UNESP] Ribeiro, Márcio Garcia [UNESP] de Carvalho Azevedo, Vasco Ariston Borges, Alexandre Secorun [UNESP] Fernandes-Júnior, Ary [UNESP] Oliveira-Filho, José Paes [UNESP] |
author_role |
author |
author2 |
de Paula, Carolina Lechinski [UNESP] Pereira, Ana Flávia Marques [UNESP] Ribeiro, Márcio Garcia [UNESP] de Carvalho Azevedo, Vasco Ariston Borges, Alexandre Secorun [UNESP] Fernandes-Júnior, Ary [UNESP] Oliveira-Filho, José Paes [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of California – Davis Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Bernardino, Pedro Negri [UNESP] de Paula, Carolina Lechinski [UNESP] Pereira, Ana Flávia Marques [UNESP] Ribeiro, Márcio Garcia [UNESP] de Carvalho Azevedo, Vasco Ariston Borges, Alexandre Secorun [UNESP] Fernandes-Júnior, Ary [UNESP] Oliveira-Filho, José Paes [UNESP] |
dc.subject.por.fl_str_mv |
2’,3’-Dideoxyadenosine Bactericidal Bacteriostatic Caseous lymphadenitis Small ruminant |
topic |
2’,3’-Dideoxyadenosine Bactericidal Bacteriostatic Caseous lymphadenitis Small ruminant |
description |
Caseous lymphadenitis is a well-known disease caused by Corynebacterium pseudotuberculosis affecting small ruminants with small significance to human health because of its minor zoonotic potential. In both cases, few treatment options are available and conventional antimicrobial therapy is commonly refractory due to development of pyogranulomatous reactions, bringing great interest in discovering novel therapeutics for more suitable approaches. Dideoxynucleotides presented antibacterial action against various bacteria but were never described for C. pseudotuberculosis. Hypothesizing the antimicrobial action of 2’,3’-dideoxiadenosine (ddATP) against C. pseudotuberculosis, we performed for the first time an investigation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in the ATCC® 19,410 strain and a well-characterized clinical isolate of C. pseudotuberculosis. We also assessed potential synergism with penicillin. ddATP showed a growth delay effect for C. pseudotuberculosis at 2 µmol/mL and a MIC and MBC of 4 µmol/mL against the ATCC® 19,410 strain, but not for the clinical strain. An antimicrobial effect was observed when using concentrations lower than the MIC of ddATP associated with penicillin for both strains tested. Our data suggest the potential of nucleotide analogs, especially adenosine, and its combination with penicillin, as a possible novel treatment for C. pseudotuberculosis-induced infections, and contributes with knowledge regarding alternative drugs to treat C. pseudotuberculosis infections. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T15:42:32Z 2023-07-29T15:42:32Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s42770-022-00885-0 Brazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023. 1678-4405 1517-8382 http://hdl.handle.net/11449/249483 10.1007/s42770-022-00885-0 2-s2.0-85144136740 |
url |
http://dx.doi.org/10.1007/s42770-022-00885-0 http://hdl.handle.net/11449/249483 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 54, n. 1, p. 559-563, 2023. 1678-4405 1517-8382 10.1007/s42770-022-00885-0 2-s2.0-85144136740 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
559-563 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965530291961856 |