Análise citogenética em espécies de Scinax pertencentes aos clados de S. catharinae e S. ruber, com o uso de técnicas de citogenética clássica e molecular

• Autor(a) principal: Oliveira, Gabriela Isabela Gomes de [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156440; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-04-26/000897904.pdf
• topic: Genetica animal; Citogenética animal; Hylidae; Cromossomos

The Scinax genus, which is distributed throughout the American continent from Mexico to Argentina and Uruguay, includes small sized animals known as tree frogs. From the taxonomy point of view, the genus belongs to the Dendropsophini tribe, Hylinae subfamily, Hylidae family and, according to extensive revisions in the family phylogeny, their representatives are currently distributed in two major clades of S. catharinae and S. ruber. The Scinax representatives which had their chromosomes analyzed so far showed 2n = 24, but different karyotypic patterns are observed for each clade, according to the morphology of the first two pairs, Ag-NOR position and the amount of C banded heterochromatin. In this study, we are presenting the cytogenetic data of 11 species of Scinax, three of them analyzed for the first time, five from the clade of S. catharinae (S. albicans, S. argyreornatus, S. hiemalis, S. littoralis and Scinax sp. gr. catharinae) and six from the clade of S. ruber (S. caldarum, S. crospedospilus, S. eurydice, S. fuscovarius, S. hayii and S. similis). The aim was to find new chromosome markers to better characterize both clades. The results of Giemsa staining and Ag-NOR comfirmed the the previous observed patterns for each clade. Replication bands obtained in this work for the species S. hiemalis, S. littoralis, S. crospedospilus, and S. eurydice were compared to those previously obtained in our laboratory for the species S. fuscovarius and S. similis, allowing to confirm that the observed differences with the use of conventional staining among species of distinct clades seem to be due to deletions on the short arms of chromosomes from pairs 1 and 2. The remaining chromosomes especially those of large size showed equivalent banding patterns. The C banding technique revealed on both clades subtle small bands, which only in S. crospedospilus, S. hayii, and S. littoralis appeared marked with...