Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ejbt.2019.10.003 http://hdl.handle.net/11449/196350 |
Resumo: | Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes. Results: Under infective conditions, 446 genes were up-and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up-and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence. Conclusions: Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac. (C) 2019 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved. |
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Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citriCitrus sinensisDNA microarraysGene expressionHypothetical genesOligonucleotide Array Sequence AnalysisRT-qPCRTranscriptomeType III secretion systemsVirulenceXanthan gumBackground: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes. Results: Under infective conditions, 446 genes were up-and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up-and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence. Conclusions: Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac. (C) 2019 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fund for Citrus Plant Protection (FUNDECITRUS)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Vales do Jequitirthonha e Mucuri, Fac Ciencias Agr, Dept Engn Florestal, Diamantina, MG, BrazilUniv Fed Ouro Preto, Inst Ciencias Exatas & Biol, Dept Ciencias Biol, Ouro Preto, MG, BrazilUniv Fed Ouro Preto, Ouro Preto, Nucleo Pesquisas Ciencias Biol, Ouro Preto, MG, BrazilUniv Fed Vales do Jequitirthonha e Mucuri, Inst Ciencias Agr, Unai, MG, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Campus Jaboticabal, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Campus Jaboticabal, Jaboticabal, SP, BrazilFAPESP: 02/13862-6FAPEMIG: CBB-APQ-04425-10CAPES: 001Univ Catolica De ValparaisoUniv Fed Vales do Jequitirthonha e MucuriUniv Fed Ouro PretoUniversidade Estadual Paulista (Unesp)Laia, Marcelo Luiz deMoreira, Leandro MarcioGoncalves, Janaina FernandesTiraboschi Ferro, Maria Ines [UNESP]Pinto Rodrigues, Any CarolinySantos, Jessica Naiara dosFelestrino, Erica BarbosaFerro, Jesus Aparecido [UNESP]2020-12-10T19:41:49Z2020-12-10T19:41:49Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article30-41http://dx.doi.org/10.1016/j.ejbt.2019.10.003Electronic Journal Of Biotechnology. Valparaiso: Univ Catolica De Valparaiso, v. 42, p. 30-41, 2019.0717-3458http://hdl.handle.net/11449/19635010.1016/j.ejbt.2019.10.003WOS:000498892600005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengElectronic Journal Of Biotechnologyinfo:eu-repo/semantics/openAccess2021-10-23T07:14:43Zoai:repositorio.unesp.br:11449/196350Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T07:14:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
title |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
spellingShingle |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri Laia, Marcelo Luiz de Citrus sinensis DNA microarrays Gene expression Hypothetical genes Oligonucleotide Array Sequence Analysis RT-qPCR Transcriptome Type III secretion systems Virulence Xanthan gum |
title_short |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
title_full |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
title_fullStr |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
title_full_unstemmed |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
title_sort |
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri |
author |
Laia, Marcelo Luiz de |
author_facet |
Laia, Marcelo Luiz de Moreira, Leandro Marcio Goncalves, Janaina Fernandes Tiraboschi Ferro, Maria Ines [UNESP] Pinto Rodrigues, Any Caroliny Santos, Jessica Naiara dos Felestrino, Erica Barbosa Ferro, Jesus Aparecido [UNESP] |
author_role |
author |
author2 |
Moreira, Leandro Marcio Goncalves, Janaina Fernandes Tiraboschi Ferro, Maria Ines [UNESP] Pinto Rodrigues, Any Caroliny Santos, Jessica Naiara dos Felestrino, Erica Barbosa Ferro, Jesus Aparecido [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Vales do Jequitirthonha e Mucuri Univ Fed Ouro Preto Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Laia, Marcelo Luiz de Moreira, Leandro Marcio Goncalves, Janaina Fernandes Tiraboschi Ferro, Maria Ines [UNESP] Pinto Rodrigues, Any Caroliny Santos, Jessica Naiara dos Felestrino, Erica Barbosa Ferro, Jesus Aparecido [UNESP] |
dc.subject.por.fl_str_mv |
Citrus sinensis DNA microarrays Gene expression Hypothetical genes Oligonucleotide Array Sequence Analysis RT-qPCR Transcriptome Type III secretion systems Virulence Xanthan gum |
topic |
Citrus sinensis DNA microarrays Gene expression Hypothetical genes Oligonucleotide Array Sequence Analysis RT-qPCR Transcriptome Type III secretion systems Virulence Xanthan gum |
description |
Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes. Results: Under infective conditions, 446 genes were up-and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up-and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence. Conclusions: Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac. (C) 2019 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2020-12-10T19:41:49Z 2020-12-10T19:41:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ejbt.2019.10.003 Electronic Journal Of Biotechnology. Valparaiso: Univ Catolica De Valparaiso, v. 42, p. 30-41, 2019. 0717-3458 http://hdl.handle.net/11449/196350 10.1016/j.ejbt.2019.10.003 WOS:000498892600005 |
url |
http://dx.doi.org/10.1016/j.ejbt.2019.10.003 http://hdl.handle.net/11449/196350 |
identifier_str_mv |
Electronic Journal Of Biotechnology. Valparaiso: Univ Catolica De Valparaiso, v. 42, p. 30-41, 2019. 0717-3458 10.1016/j.ejbt.2019.10.003 WOS:000498892600005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Electronic Journal Of Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
30-41 |
dc.publisher.none.fl_str_mv |
Univ Catolica De Valparaiso |
publisher.none.fl_str_mv |
Univ Catolica De Valparaiso |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964981422194688 |