Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10658-021-02212-z http://hdl.handle.net/11449/208340 |
Resumo: | In Brazil, Sclerotinia stem rot (SSR) control in soybean depends primarily on application of the fungicides carbendazim, fluazinam, and procymidone. While these fungicides can provide effective control of the disease, their protection period, which is crucial for timing their optimal application, is poorly understood. In the present study, the protection period of carbendazim, fluazinam and procymidone was determined using a detached leaf bioassay (DLB) and chromatography. For the DLB, soybean plants were treated twice with each fungicide with or without mineral oil as adjuvant; leaves were collected at two or three-day intervals from 11 to 23 days, inoculated with Sclerotinia sclerotiorum mycelium, and the necrotic area was assessed 48 h after inoculation. For the chromatography test, each fungicide was applied and the leaves were collected every two days after treatment for 14 days. Fungicide residue concentration in tissue was determined according to a modified version of the quick, easy, cheap, effective, rugged and safe (QuEChERS) method. At 5, 7, and 9 days after the first application, fluazinam, carbendazim and procymidone, respectively, had no protective effect even when applied with mineral oil. Greater control efficacy was achieved after a second application. Fluazinam showed protective activity up to 4 days while carbendazim and procymidone showed about 10 days. The mineral oil only enhanced protection activity of procymidone in the second application. The analytical chemical separation indicated that the relative concentration of fluazinam decreased at a faster rate than that of carbendazim and procymidone. At day 14, the concentration of carbendazim and procymidone decreased from 34.97 to 9.39 mg kg−1, and 58.98 to 11.22 mg kg−1, respectively, suggesting that 10–14 days represent the approximate timing in spacing two consecutive applications. The knowledge of the protection period of these fungicides sets the basis to improving their timing of applications. |
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Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatographyCarbendazimFluazinamGlycine maxMineral oilProcymidoneSclerotinia sclerotiorumIn Brazil, Sclerotinia stem rot (SSR) control in soybean depends primarily on application of the fungicides carbendazim, fluazinam, and procymidone. While these fungicides can provide effective control of the disease, their protection period, which is crucial for timing their optimal application, is poorly understood. In the present study, the protection period of carbendazim, fluazinam and procymidone was determined using a detached leaf bioassay (DLB) and chromatography. For the DLB, soybean plants were treated twice with each fungicide with or without mineral oil as adjuvant; leaves were collected at two or three-day intervals from 11 to 23 days, inoculated with Sclerotinia sclerotiorum mycelium, and the necrotic area was assessed 48 h after inoculation. For the chromatography test, each fungicide was applied and the leaves were collected every two days after treatment for 14 days. Fungicide residue concentration in tissue was determined according to a modified version of the quick, easy, cheap, effective, rugged and safe (QuEChERS) method. At 5, 7, and 9 days after the first application, fluazinam, carbendazim and procymidone, respectively, had no protective effect even when applied with mineral oil. Greater control efficacy was achieved after a second application. Fluazinam showed protective activity up to 4 days while carbendazim and procymidone showed about 10 days. The mineral oil only enhanced protection activity of procymidone in the second application. The analytical chemical separation indicated that the relative concentration of fluazinam decreased at a faster rate than that of carbendazim and procymidone. At day 14, the concentration of carbendazim and procymidone decreased from 34.97 to 9.39 mg kg−1, and 58.98 to 11.22 mg kg−1, respectively, suggesting that 10–14 days represent the approximate timing in spacing two consecutive applications. The knowledge of the protection period of these fungicides sets the basis to improving their timing of applications.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Carrington Research Extension Center North Dakota State UniversityDepartment of Plant Protection School of Agriculture São Paulo State University (UNESP)School of Agricultural Sciences Northwest Missouri State UniversityDepartment of Plant Protection School of Agriculture São Paulo State University (UNESP)CAPES: 001North Dakota State UniversityUniversidade Estadual Paulista (Unesp)Northwest Missouri State UniversityMiorini, Thomas J. J.Raetano, Carlos Gilberto [UNESP]Negrisoli, Matheus M. [UNESP]Pérez-Hernández, Oscar2021-06-25T11:10:34Z2021-06-25T11:10:34Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article877-889http://dx.doi.org/10.1007/s10658-021-02212-zEuropean Journal of Plant Pathology, v. 159, n. 4, p. 877-889, 2021.1573-84690929-1873http://hdl.handle.net/11449/20834010.1007/s10658-021-02212-z2-s2.0-85099753948Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Plant Pathologyinfo:eu-repo/semantics/openAccess2024-04-30T18:07:43Zoai:repositorio.unesp.br:11449/208340Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:32:51.203304Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
title |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
spellingShingle |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography Miorini, Thomas J. J. Carbendazim Fluazinam Glycine max Mineral oil Procymidone Sclerotinia sclerotiorum |
title_short |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
title_full |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
title_fullStr |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
title_full_unstemmed |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
title_sort |
Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography |
author |
Miorini, Thomas J. J. |
author_facet |
Miorini, Thomas J. J. Raetano, Carlos Gilberto [UNESP] Negrisoli, Matheus M. [UNESP] Pérez-Hernández, Oscar |
author_role |
author |
author2 |
Raetano, Carlos Gilberto [UNESP] Negrisoli, Matheus M. [UNESP] Pérez-Hernández, Oscar |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
North Dakota State University Universidade Estadual Paulista (Unesp) Northwest Missouri State University |
dc.contributor.author.fl_str_mv |
Miorini, Thomas J. J. Raetano, Carlos Gilberto [UNESP] Negrisoli, Matheus M. [UNESP] Pérez-Hernández, Oscar |
dc.subject.por.fl_str_mv |
Carbendazim Fluazinam Glycine max Mineral oil Procymidone Sclerotinia sclerotiorum |
topic |
Carbendazim Fluazinam Glycine max Mineral oil Procymidone Sclerotinia sclerotiorum |
description |
In Brazil, Sclerotinia stem rot (SSR) control in soybean depends primarily on application of the fungicides carbendazim, fluazinam, and procymidone. While these fungicides can provide effective control of the disease, their protection period, which is crucial for timing their optimal application, is poorly understood. In the present study, the protection period of carbendazim, fluazinam and procymidone was determined using a detached leaf bioassay (DLB) and chromatography. For the DLB, soybean plants were treated twice with each fungicide with or without mineral oil as adjuvant; leaves were collected at two or three-day intervals from 11 to 23 days, inoculated with Sclerotinia sclerotiorum mycelium, and the necrotic area was assessed 48 h after inoculation. For the chromatography test, each fungicide was applied and the leaves were collected every two days after treatment for 14 days. Fungicide residue concentration in tissue was determined according to a modified version of the quick, easy, cheap, effective, rugged and safe (QuEChERS) method. At 5, 7, and 9 days after the first application, fluazinam, carbendazim and procymidone, respectively, had no protective effect even when applied with mineral oil. Greater control efficacy was achieved after a second application. Fluazinam showed protective activity up to 4 days while carbendazim and procymidone showed about 10 days. The mineral oil only enhanced protection activity of procymidone in the second application. The analytical chemical separation indicated that the relative concentration of fluazinam decreased at a faster rate than that of carbendazim and procymidone. At day 14, the concentration of carbendazim and procymidone decreased from 34.97 to 9.39 mg kg−1, and 58.98 to 11.22 mg kg−1, respectively, suggesting that 10–14 days represent the approximate timing in spacing two consecutive applications. The knowledge of the protection period of these fungicides sets the basis to improving their timing of applications. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:10:34Z 2021-06-25T11:10:34Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10658-021-02212-z European Journal of Plant Pathology, v. 159, n. 4, p. 877-889, 2021. 1573-8469 0929-1873 http://hdl.handle.net/11449/208340 10.1007/s10658-021-02212-z 2-s2.0-85099753948 |
url |
http://dx.doi.org/10.1007/s10658-021-02212-z http://hdl.handle.net/11449/208340 |
identifier_str_mv |
European Journal of Plant Pathology, v. 159, n. 4, p. 877-889, 2021. 1573-8469 0929-1873 10.1007/s10658-021-02212-z 2-s2.0-85099753948 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Plant Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
877-889 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129332635238400 |