Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4081/ejh.2021.3274 http://hdl.handle.net/11449/222148 |
Resumo: | The vertical ladder-based protocols contribute to the NMJ junction's adaptations, and when combined with and without load, can be potentiated. The present study aimed to investigate postsynaptic regions of the biceps brachii muscle in adult male Wistar rats submitted to different vertical ladder-based protocols (Sedentary-S; Climbing-C; Climbing with Load-LC and Combined Climbing-CC). The protocols (C, LC, CC) were per-formed in 24 sessions, 3 x/week, for 8 weeks. The myofibrillar ATPase analysis showed an increase in cross-sectional area (CSA) of the muscle fibers Type I in all trained Groups; Type II in C and LC and reduction in CC; Type IIx higher in all trained Groups. In the postsynaptic cleft, the stained area presents smaller in Groups C, LC, and CC; the total area showed smaller than LC and higher in C and CC. The stained and total perimeter, and dispersion showed a reduction in C, LC, and CC, higher maximum diameter in Groups C and CC, and decreased in LC. Regarding the postsynaptic cleft distribution, the stained area presented a decrease in all trained Groups. The integrated density presented higher principally in CC. The NMJ count showed an increase in all trained Groups. We concluded that the vertical ladder-based protocols combined contributed to the post-synaptic region adaptations. |
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Repositório Institucional da UNESP |
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Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophyHypertrophyImmunohisto-chemistryMorphometryMuscle fiber typeNeuromuscular junctionSynapseThe vertical ladder-based protocols contribute to the NMJ junction's adaptations, and when combined with and without load, can be potentiated. The present study aimed to investigate postsynaptic regions of the biceps brachii muscle in adult male Wistar rats submitted to different vertical ladder-based protocols (Sedentary-S; Climbing-C; Climbing with Load-LC and Combined Climbing-CC). The protocols (C, LC, CC) were per-formed in 24 sessions, 3 x/week, for 8 weeks. The myofibrillar ATPase analysis showed an increase in cross-sectional area (CSA) of the muscle fibers Type I in all trained Groups; Type II in C and LC and reduction in CC; Type IIx higher in all trained Groups. In the postsynaptic cleft, the stained area presents smaller in Groups C, LC, and CC; the total area showed smaller than LC and higher in C and CC. The stained and total perimeter, and dispersion showed a reduction in C, LC, and CC, higher maximum diameter in Groups C and CC, and decreased in LC. Regarding the postsynaptic cleft distribution, the stained area presented a decrease in all trained Groups. The integrated density presented higher principally in CC. The NMJ count showed an increase in all trained Groups. We concluded that the vertical ladder-based protocols combined contributed to the post-synaptic region adaptations.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences (IB) São Paulo State University (UNESP)Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences (IB) São Paulo State University (UNESP)CAPES: 001FAPESP: 2017/12525-1FAPESP: 2017/21977-3Universidade Estadual Paulista (UNESP)Neto, Jurandyr Pimentel [UNESP]Rocha, Lara Caetano [UNESP]Jacob, Carolina Dos Santos [UNESP]Barbosa, Gabriela Klein [UNESP]Ciena, Adriano Polican [UNESP]2022-04-28T19:42:41Z2022-04-28T19:42:41Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4081/ejh.2021.3274European Journal of Histochemistry, v. 65, n. S1, 2021.2038-83061121-760Xhttp://hdl.handle.net/11449/22214810.4081/ejh.2021.32742-s2.0-85112108771Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Histochemistryinfo:eu-repo/semantics/openAccess2022-04-28T19:42:41Zoai:repositorio.unesp.br:11449/222148Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:46:41.989486Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
title |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
spellingShingle |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy Neto, Jurandyr Pimentel [UNESP] Hypertrophy Immunohisto-chemistry Morphometry Muscle fiber type Neuromuscular junction Synapse |
title_short |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
title_full |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
title_fullStr |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
title_full_unstemmed |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
title_sort |
Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy |
author |
Neto, Jurandyr Pimentel [UNESP] |
author_facet |
Neto, Jurandyr Pimentel [UNESP] Rocha, Lara Caetano [UNESP] Jacob, Carolina Dos Santos [UNESP] Barbosa, Gabriela Klein [UNESP] Ciena, Adriano Polican [UNESP] |
author_role |
author |
author2 |
Rocha, Lara Caetano [UNESP] Jacob, Carolina Dos Santos [UNESP] Barbosa, Gabriela Klein [UNESP] Ciena, Adriano Polican [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Neto, Jurandyr Pimentel [UNESP] Rocha, Lara Caetano [UNESP] Jacob, Carolina Dos Santos [UNESP] Barbosa, Gabriela Klein [UNESP] Ciena, Adriano Polican [UNESP] |
dc.subject.por.fl_str_mv |
Hypertrophy Immunohisto-chemistry Morphometry Muscle fiber type Neuromuscular junction Synapse |
topic |
Hypertrophy Immunohisto-chemistry Morphometry Muscle fiber type Neuromuscular junction Synapse |
description |
The vertical ladder-based protocols contribute to the NMJ junction's adaptations, and when combined with and without load, can be potentiated. The present study aimed to investigate postsynaptic regions of the biceps brachii muscle in adult male Wistar rats submitted to different vertical ladder-based protocols (Sedentary-S; Climbing-C; Climbing with Load-LC and Combined Climbing-CC). The protocols (C, LC, CC) were per-formed in 24 sessions, 3 x/week, for 8 weeks. The myofibrillar ATPase analysis showed an increase in cross-sectional area (CSA) of the muscle fibers Type I in all trained Groups; Type II in C and LC and reduction in CC; Type IIx higher in all trained Groups. In the postsynaptic cleft, the stained area presents smaller in Groups C, LC, and CC; the total area showed smaller than LC and higher in C and CC. The stained and total perimeter, and dispersion showed a reduction in C, LC, and CC, higher maximum diameter in Groups C and CC, and decreased in LC. Regarding the postsynaptic cleft distribution, the stained area presented a decrease in all trained Groups. The integrated density presented higher principally in CC. The NMJ count showed an increase in all trained Groups. We concluded that the vertical ladder-based protocols combined contributed to the post-synaptic region adaptations. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-01 2022-04-28T19:42:41Z 2022-04-28T19:42:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4081/ejh.2021.3274 European Journal of Histochemistry, v. 65, n. S1, 2021. 2038-8306 1121-760X http://hdl.handle.net/11449/222148 10.4081/ejh.2021.3274 2-s2.0-85112108771 |
url |
http://dx.doi.org/10.4081/ejh.2021.3274 http://hdl.handle.net/11449/222148 |
identifier_str_mv |
European Journal of Histochemistry, v. 65, n. S1, 2021. 2038-8306 1121-760X 10.4081/ejh.2021.3274 2-s2.0-85112108771 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Histochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128561641422848 |