Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2019.09.020 http://hdl.handle.net/11449/197534 |
Resumo: | Differences in reproductive physiology between cattle breeds may help to explain distinct responses to assisted reproductive techniques and to define breed-specific protocols with improved efficiency. Germinal vesicle (GV) stage oocytes are characterized by increasing levels of chromatin compaction enclosed within the nucleus (graded from GV0 to GV3), associated with different developmental competence. The first objective of this study was to characterize chromatin configuration of GV stage oocytes recovered by OPU at random days of the estrous cycle from Nelore (Bos indices) and Holstein (Bos taurus) cows. In Nelore 90% of the oocytes presented advanced stages of chromatin compaction associated with higher developmental competence (GV2 and GV3), while in Holstein, only 65% of the oocytes were at these stages. Then, aiming to obtain a more homogeneous population of oocytes in Holstein, we tested two synchronization protocols combining aspiration of all visible follicles at a random day (day 0), two IM injections of FSH 12 h apart on day 2, and OPU on day 4 (OPU/D4) or 5 (OPU/D5). The protocol OPU/D4 provided around 45% of the oocytes with low chromatin compaction (GV1), while the protocol OPU/D5 provided 70% of the oocytes at GV2 and 20% at GV3. Finally, we assessed the effects of a culture system known to prevent meiotic resumption on chromatin configuration of the GV2 enriched oocyte population obtained with the protocol OPU/D5. After 9 h of culture most oocytes transited from GV2 to GV3, with 90% of the oocytes at GV3 stage. This study demonstrates differences between Nelore and Holstein cows regarding patterns of chromatin configuration that may account for their different performance in IVM/IVF. In addition, it provides novel references for the design of protocols aiming to regulate oocyte quality before IVM for the optimization of IVF outcomes. (C) 2019 Elsevier Inc. All rights reserved. |
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Characterization and control of oocyte large-scale chromatin configuration in different cattle breedsOPUFollicle synchronizationGerminal vesiclePre-maturationNPPCEstradiolProgesteroneTestosteroneDifferences in reproductive physiology between cattle breeds may help to explain distinct responses to assisted reproductive techniques and to define breed-specific protocols with improved efficiency. Germinal vesicle (GV) stage oocytes are characterized by increasing levels of chromatin compaction enclosed within the nucleus (graded from GV0 to GV3), associated with different developmental competence. The first objective of this study was to characterize chromatin configuration of GV stage oocytes recovered by OPU at random days of the estrous cycle from Nelore (Bos indices) and Holstein (Bos taurus) cows. In Nelore 90% of the oocytes presented advanced stages of chromatin compaction associated with higher developmental competence (GV2 and GV3), while in Holstein, only 65% of the oocytes were at these stages. Then, aiming to obtain a more homogeneous population of oocytes in Holstein, we tested two synchronization protocols combining aspiration of all visible follicles at a random day (day 0), two IM injections of FSH 12 h apart on day 2, and OPU on day 4 (OPU/D4) or 5 (OPU/D5). The protocol OPU/D4 provided around 45% of the oocytes with low chromatin compaction (GV1), while the protocol OPU/D5 provided 70% of the oocytes at GV2 and 20% at GV3. Finally, we assessed the effects of a culture system known to prevent meiotic resumption on chromatin configuration of the GV2 enriched oocyte population obtained with the protocol OPU/D5. After 9 h of culture most oocytes transited from GV2 to GV3, with 90% of the oocytes at GV3 stage. This study demonstrates differences between Nelore and Holstein cows regarding patterns of chromatin configuration that may account for their different performance in IVM/IVF. In addition, it provides novel references for the design of protocols aiming to regulate oocyte quality before IVM for the optimization of IVF outcomes. (C) 2019 Elsevier Inc. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Biosci, Dept Physiol, Ovarian Mol Physiol Lab, Sao Paulo, BrazilUniv Sao Paulo, Dept Anim Reprod, Sao Paulo, BrazilUniv Milan, Dept Hlth Anim Sci & Food Safety, Reprod & Dev Biol Lab, Milan, ItalySao Paulo State Univ, Inst Biosci, Dept Physiol, Ovarian Mol Physiol Lab, Sao Paulo, BrazilCAPES: 001FAPESP: 2017/07588-4FAPESP: 2016/21671-9Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Univ MilanSilva Soares, Ana Caroline [UNESP]Sakoda, Jhessica Naomi [UNESP]Gama, Isabela Lima [UNESP]Bayeux, Bernardo MarcozziLodde, ValentinaLuciano, Alberto MariaBuratini, Jose [UNESP]2020-12-11T01:57:24Z2020-12-11T01:57:24Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article146-152http://dx.doi.org/10.1016/j.theriogenology.2019.09.020Theriogenology. New York: Elsevier Science Inc, v. 141, p. 146-152, 2020.0093-691Xhttp://hdl.handle.net/11449/19753410.1016/j.theriogenology.2019.09.020WOS:000494049100020Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2021-10-23T04:53:47Zoai:repositorio.unesp.br:11449/197534Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:22:44.241952Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
title |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
spellingShingle |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds Silva Soares, Ana Caroline [UNESP] OPU Follicle synchronization Germinal vesicle Pre-maturation NPPC Estradiol Progesterone Testosterone |
title_short |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
title_full |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
title_fullStr |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
title_full_unstemmed |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
title_sort |
Characterization and control of oocyte large-scale chromatin configuration in different cattle breeds |
author |
Silva Soares, Ana Caroline [UNESP] |
author_facet |
Silva Soares, Ana Caroline [UNESP] Sakoda, Jhessica Naomi [UNESP] Gama, Isabela Lima [UNESP] Bayeux, Bernardo Marcozzi Lodde, Valentina Luciano, Alberto Maria Buratini, Jose [UNESP] |
author_role |
author |
author2 |
Sakoda, Jhessica Naomi [UNESP] Gama, Isabela Lima [UNESP] Bayeux, Bernardo Marcozzi Lodde, Valentina Luciano, Alberto Maria Buratini, Jose [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Univ Milan |
dc.contributor.author.fl_str_mv |
Silva Soares, Ana Caroline [UNESP] Sakoda, Jhessica Naomi [UNESP] Gama, Isabela Lima [UNESP] Bayeux, Bernardo Marcozzi Lodde, Valentina Luciano, Alberto Maria Buratini, Jose [UNESP] |
dc.subject.por.fl_str_mv |
OPU Follicle synchronization Germinal vesicle Pre-maturation NPPC Estradiol Progesterone Testosterone |
topic |
OPU Follicle synchronization Germinal vesicle Pre-maturation NPPC Estradiol Progesterone Testosterone |
description |
Differences in reproductive physiology between cattle breeds may help to explain distinct responses to assisted reproductive techniques and to define breed-specific protocols with improved efficiency. Germinal vesicle (GV) stage oocytes are characterized by increasing levels of chromatin compaction enclosed within the nucleus (graded from GV0 to GV3), associated with different developmental competence. The first objective of this study was to characterize chromatin configuration of GV stage oocytes recovered by OPU at random days of the estrous cycle from Nelore (Bos indices) and Holstein (Bos taurus) cows. In Nelore 90% of the oocytes presented advanced stages of chromatin compaction associated with higher developmental competence (GV2 and GV3), while in Holstein, only 65% of the oocytes were at these stages. Then, aiming to obtain a more homogeneous population of oocytes in Holstein, we tested two synchronization protocols combining aspiration of all visible follicles at a random day (day 0), two IM injections of FSH 12 h apart on day 2, and OPU on day 4 (OPU/D4) or 5 (OPU/D5). The protocol OPU/D4 provided around 45% of the oocytes with low chromatin compaction (GV1), while the protocol OPU/D5 provided 70% of the oocytes at GV2 and 20% at GV3. Finally, we assessed the effects of a culture system known to prevent meiotic resumption on chromatin configuration of the GV2 enriched oocyte population obtained with the protocol OPU/D5. After 9 h of culture most oocytes transited from GV2 to GV3, with 90% of the oocytes at GV3 stage. This study demonstrates differences between Nelore and Holstein cows regarding patterns of chromatin configuration that may account for their different performance in IVM/IVF. In addition, it provides novel references for the design of protocols aiming to regulate oocyte quality before IVM for the optimization of IVF outcomes. (C) 2019 Elsevier Inc. All rights reserved. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-11T01:57:24Z 2020-12-11T01:57:24Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2019.09.020 Theriogenology. New York: Elsevier Science Inc, v. 141, p. 146-152, 2020. 0093-691X http://hdl.handle.net/11449/197534 10.1016/j.theriogenology.2019.09.020 WOS:000494049100020 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2019.09.020 http://hdl.handle.net/11449/197534 |
identifier_str_mv |
Theriogenology. New York: Elsevier Science Inc, v. 141, p. 146-152, 2020. 0093-691X 10.1016/j.theriogenology.2019.09.020 WOS:000494049100020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
146-152 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128802019082240 |