Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke

Detalhes bibliográficos
Autor(a) principal: Moreno, Ana Caroline Rippi [UNESP]
Data de Publicação: 2022
Outros Autores: Olean-Oliveira, André [UNESP], Olean-Oliveira, Tiago [UNESP], Nunes, Maria Tereza, Teixeira, Marcos F.S. [UNESP], Seraphim, Patricia Monteiro [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.lfs.2022.121017
http://hdl.handle.net/11449/249213
Resumo: Aim: To analyze the consumption of oxygen and to quantify the mitochondrial respiratory chain proteins (OXPHOS) in the gastrocnemius muscle of rats exposed to cigarette smoke and/or RT practitioners. Main methods: Wistar rats were divided into groups: Control (C), Smoker (S), Exercise (E) and Exercise Smoker (ES). Groups F and ES were exposed to the smoke of 4 cigarettes for 30 min, 2× a day, 5× a week, for 16 weeks. Groups E and ES performed four climbs with progressive load, 1× per day, 5× per week, for 16 weeks. The gastrocnemius muscle was collected for analysis of OXPHOS content and oxygen consumption. Groups S (vs. C) and ES (vs. C and E) showed lower body weight gain when observing the evolution curve. Key findings: The S rats showed a reduction in the NDUFB8 proteins of complex 1, SDHB of complex 2, MTC01 of complex 4 and ATP5A of complex 5 (ATP Synthase) compared to Group C. Additionally, S rats also showed increased consumption of O2 in Basal, Leak, Complex I and I/II combined measures compared to the other groups, suggesting that the activity of the mitochondria of these animals increased in terms of coupling and uncoupling parameters. Significance: Our data suggest that exposure to cigarette smoke for 16 weeks is capable of causing impairment of mitochondrial function with reduced expression of respiratory chain proteins in skeletal muscle. However, the RT was effective in preventing impairment of mitochondrial function in the skeletal muscle of rats exposed to secondary cigarette smoke.
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spelling Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smokeMitochondriaResistive exerciseRespiratory chainSkeletal muscleSmokingAim: To analyze the consumption of oxygen and to quantify the mitochondrial respiratory chain proteins (OXPHOS) in the gastrocnemius muscle of rats exposed to cigarette smoke and/or RT practitioners. Main methods: Wistar rats were divided into groups: Control (C), Smoker (S), Exercise (E) and Exercise Smoker (ES). Groups F and ES were exposed to the smoke of 4 cigarettes for 30 min, 2× a day, 5× a week, for 16 weeks. Groups E and ES performed four climbs with progressive load, 1× per day, 5× per week, for 16 weeks. The gastrocnemius muscle was collected for analysis of OXPHOS content and oxygen consumption. Groups S (vs. C) and ES (vs. C and E) showed lower body weight gain when observing the evolution curve. Key findings: The S rats showed a reduction in the NDUFB8 proteins of complex 1, SDHB of complex 2, MTC01 of complex 4 and ATP5A of complex 5 (ATP Synthase) compared to Group C. Additionally, S rats also showed increased consumption of O2 in Basal, Leak, Complex I and I/II combined measures compared to the other groups, suggesting that the activity of the mitochondria of these animals increased in terms of coupling and uncoupling parameters. Significance: Our data suggest that exposure to cigarette smoke for 16 weeks is capable of causing impairment of mitochondrial function with reduced expression of respiratory chain proteins in skeletal muscle. However, the RT was effective in preventing impairment of mitochondrial function in the skeletal muscle of rats exposed to secondary cigarette smoke.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physiotherapy School of Sciences and Technology Sao Paulo State University (UNESP), SPDepartment of Physiology and Biophysics – Institute of Biomedical Sciences I University of Sao Paulo, SPDepartment of Chemistry and Biochemistry School of Sciences and Technology Sao Paulo State University (UNESP), SPDepartment of Physiotherapy School of Sciences and Technology Sao Paulo State University (UNESP), SPDepartment of Chemistry and Biochemistry School of Sciences and Technology Sao Paulo State University (UNESP), SPFAPESP: 2013/05629-4CNPq: 303282/2020-7Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Moreno, Ana Caroline Rippi [UNESP]Olean-Oliveira, André [UNESP]Olean-Oliveira, Tiago [UNESP]Nunes, Maria TerezaTeixeira, Marcos F.S. [UNESP]Seraphim, Patricia Monteiro [UNESP]2023-07-29T14:13:21Z2023-07-29T14:13:21Z2022-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lfs.2022.121017Life Sciences, v. 309.1879-06310024-3205http://hdl.handle.net/11449/24921310.1016/j.lfs.2022.1210172-s2.0-85139007107Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2024-06-18T18:44:14Zoai:repositorio.unesp.br:11449/249213Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:44.799164Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
title Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
spellingShingle Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
Moreno, Ana Caroline Rippi [UNESP]
Mitochondria
Resistive exercise
Respiratory chain
Skeletal muscle
Smoking
title_short Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
title_full Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
title_fullStr Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
title_full_unstemmed Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
title_sort Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
author Moreno, Ana Caroline Rippi [UNESP]
author_facet Moreno, Ana Caroline Rippi [UNESP]
Olean-Oliveira, André [UNESP]
Olean-Oliveira, Tiago [UNESP]
Nunes, Maria Tereza
Teixeira, Marcos F.S. [UNESP]
Seraphim, Patricia Monteiro [UNESP]
author_role author
author2 Olean-Oliveira, André [UNESP]
Olean-Oliveira, Tiago [UNESP]
Nunes, Maria Tereza
Teixeira, Marcos F.S. [UNESP]
Seraphim, Patricia Monteiro [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Moreno, Ana Caroline Rippi [UNESP]
Olean-Oliveira, André [UNESP]
Olean-Oliveira, Tiago [UNESP]
Nunes, Maria Tereza
Teixeira, Marcos F.S. [UNESP]
Seraphim, Patricia Monteiro [UNESP]
dc.subject.por.fl_str_mv Mitochondria
Resistive exercise
Respiratory chain
Skeletal muscle
Smoking
topic Mitochondria
Resistive exercise
Respiratory chain
Skeletal muscle
Smoking
description Aim: To analyze the consumption of oxygen and to quantify the mitochondrial respiratory chain proteins (OXPHOS) in the gastrocnemius muscle of rats exposed to cigarette smoke and/or RT practitioners. Main methods: Wistar rats were divided into groups: Control (C), Smoker (S), Exercise (E) and Exercise Smoker (ES). Groups F and ES were exposed to the smoke of 4 cigarettes for 30 min, 2× a day, 5× a week, for 16 weeks. Groups E and ES performed four climbs with progressive load, 1× per day, 5× per week, for 16 weeks. The gastrocnemius muscle was collected for analysis of OXPHOS content and oxygen consumption. Groups S (vs. C) and ES (vs. C and E) showed lower body weight gain when observing the evolution curve. Key findings: The S rats showed a reduction in the NDUFB8 proteins of complex 1, SDHB of complex 2, MTC01 of complex 4 and ATP5A of complex 5 (ATP Synthase) compared to Group C. Additionally, S rats also showed increased consumption of O2 in Basal, Leak, Complex I and I/II combined measures compared to the other groups, suggesting that the activity of the mitochondria of these animals increased in terms of coupling and uncoupling parameters. Significance: Our data suggest that exposure to cigarette smoke for 16 weeks is capable of causing impairment of mitochondrial function with reduced expression of respiratory chain proteins in skeletal muscle. However, the RT was effective in preventing impairment of mitochondrial function in the skeletal muscle of rats exposed to secondary cigarette smoke.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-15
2023-07-29T14:13:21Z
2023-07-29T14:13:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.lfs.2022.121017
Life Sciences, v. 309.
1879-0631
0024-3205
http://hdl.handle.net/11449/249213
10.1016/j.lfs.2022.121017
2-s2.0-85139007107
url http://dx.doi.org/10.1016/j.lfs.2022.121017
http://hdl.handle.net/11449/249213
identifier_str_mv Life Sciences, v. 309.
1879-0631
0024-3205
10.1016/j.lfs.2022.121017
2-s2.0-85139007107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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