Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.lfs.2022.121017 http://hdl.handle.net/11449/249213 |
Resumo: | Aim: To analyze the consumption of oxygen and to quantify the mitochondrial respiratory chain proteins (OXPHOS) in the gastrocnemius muscle of rats exposed to cigarette smoke and/or RT practitioners. Main methods: Wistar rats were divided into groups: Control (C), Smoker (S), Exercise (E) and Exercise Smoker (ES). Groups F and ES were exposed to the smoke of 4 cigarettes for 30 min, 2× a day, 5× a week, for 16 weeks. Groups E and ES performed four climbs with progressive load, 1× per day, 5× per week, for 16 weeks. The gastrocnemius muscle was collected for analysis of OXPHOS content and oxygen consumption. Groups S (vs. C) and ES (vs. C and E) showed lower body weight gain when observing the evolution curve. Key findings: The S rats showed a reduction in the NDUFB8 proteins of complex 1, SDHB of complex 2, MTC01 of complex 4 and ATP5A of complex 5 (ATP Synthase) compared to Group C. Additionally, S rats also showed increased consumption of O2 in Basal, Leak, Complex I and I/II combined measures compared to the other groups, suggesting that the activity of the mitochondria of these animals increased in terms of coupling and uncoupling parameters. Significance: Our data suggest that exposure to cigarette smoke for 16 weeks is capable of causing impairment of mitochondrial function with reduced expression of respiratory chain proteins in skeletal muscle. However, the RT was effective in preventing impairment of mitochondrial function in the skeletal muscle of rats exposed to secondary cigarette smoke. |
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Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smokeMitochondriaResistive exerciseRespiratory chainSkeletal muscleSmokingAim: To analyze the consumption of oxygen and to quantify the mitochondrial respiratory chain proteins (OXPHOS) in the gastrocnemius muscle of rats exposed to cigarette smoke and/or RT practitioners. Main methods: Wistar rats were divided into groups: Control (C), Smoker (S), Exercise (E) and Exercise Smoker (ES). Groups F and ES were exposed to the smoke of 4 cigarettes for 30 min, 2× a day, 5× a week, for 16 weeks. Groups E and ES performed four climbs with progressive load, 1× per day, 5× per week, for 16 weeks. The gastrocnemius muscle was collected for analysis of OXPHOS content and oxygen consumption. Groups S (vs. C) and ES (vs. C and E) showed lower body weight gain when observing the evolution curve. Key findings: The S rats showed a reduction in the NDUFB8 proteins of complex 1, SDHB of complex 2, MTC01 of complex 4 and ATP5A of complex 5 (ATP Synthase) compared to Group C. Additionally, S rats also showed increased consumption of O2 in Basal, Leak, Complex I and I/II combined measures compared to the other groups, suggesting that the activity of the mitochondria of these animals increased in terms of coupling and uncoupling parameters. Significance: Our data suggest that exposure to cigarette smoke for 16 weeks is capable of causing impairment of mitochondrial function with reduced expression of respiratory chain proteins in skeletal muscle. However, the RT was effective in preventing impairment of mitochondrial function in the skeletal muscle of rats exposed to secondary cigarette smoke.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Physiotherapy School of Sciences and Technology Sao Paulo State University (UNESP), SPDepartment of Physiology and Biophysics – Institute of Biomedical Sciences I University of Sao Paulo, SPDepartment of Chemistry and Biochemistry School of Sciences and Technology Sao Paulo State University (UNESP), SPDepartment of Physiotherapy School of Sciences and Technology Sao Paulo State University (UNESP), SPDepartment of Chemistry and Biochemistry School of Sciences and Technology Sao Paulo State University (UNESP), SPFAPESP: 2013/05629-4CNPq: 303282/2020-7Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Moreno, Ana Caroline Rippi [UNESP]Olean-Oliveira, André [UNESP]Olean-Oliveira, Tiago [UNESP]Nunes, Maria TerezaTeixeira, Marcos F.S. [UNESP]Seraphim, Patricia Monteiro [UNESP]2023-07-29T14:13:21Z2023-07-29T14:13:21Z2022-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lfs.2022.121017Life Sciences, v. 309.1879-06310024-3205http://hdl.handle.net/11449/24921310.1016/j.lfs.2022.1210172-s2.0-85139007107Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2024-06-18T18:44:14Zoai:repositorio.unesp.br:11449/249213Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:44.799164Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
title |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
spellingShingle |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke Moreno, Ana Caroline Rippi [UNESP] Mitochondria Resistive exercise Respiratory chain Skeletal muscle Smoking |
title_short |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
title_full |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
title_fullStr |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
title_full_unstemmed |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
title_sort |
Resistance training prevents damage to the mitochondrial function of the skeletal muscle of rats exposed to secondary cigarette smoke |
author |
Moreno, Ana Caroline Rippi [UNESP] |
author_facet |
Moreno, Ana Caroline Rippi [UNESP] Olean-Oliveira, André [UNESP] Olean-Oliveira, Tiago [UNESP] Nunes, Maria Tereza Teixeira, Marcos F.S. [UNESP] Seraphim, Patricia Monteiro [UNESP] |
author_role |
author |
author2 |
Olean-Oliveira, André [UNESP] Olean-Oliveira, Tiago [UNESP] Nunes, Maria Tereza Teixeira, Marcos F.S. [UNESP] Seraphim, Patricia Monteiro [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Moreno, Ana Caroline Rippi [UNESP] Olean-Oliveira, André [UNESP] Olean-Oliveira, Tiago [UNESP] Nunes, Maria Tereza Teixeira, Marcos F.S. [UNESP] Seraphim, Patricia Monteiro [UNESP] |
dc.subject.por.fl_str_mv |
Mitochondria Resistive exercise Respiratory chain Skeletal muscle Smoking |
topic |
Mitochondria Resistive exercise Respiratory chain Skeletal muscle Smoking |
description |
Aim: To analyze the consumption of oxygen and to quantify the mitochondrial respiratory chain proteins (OXPHOS) in the gastrocnemius muscle of rats exposed to cigarette smoke and/or RT practitioners. Main methods: Wistar rats were divided into groups: Control (C), Smoker (S), Exercise (E) and Exercise Smoker (ES). Groups F and ES were exposed to the smoke of 4 cigarettes for 30 min, 2× a day, 5× a week, for 16 weeks. Groups E and ES performed four climbs with progressive load, 1× per day, 5× per week, for 16 weeks. The gastrocnemius muscle was collected for analysis of OXPHOS content and oxygen consumption. Groups S (vs. C) and ES (vs. C and E) showed lower body weight gain when observing the evolution curve. Key findings: The S rats showed a reduction in the NDUFB8 proteins of complex 1, SDHB of complex 2, MTC01 of complex 4 and ATP5A of complex 5 (ATP Synthase) compared to Group C. Additionally, S rats also showed increased consumption of O2 in Basal, Leak, Complex I and I/II combined measures compared to the other groups, suggesting that the activity of the mitochondria of these animals increased in terms of coupling and uncoupling parameters. Significance: Our data suggest that exposure to cigarette smoke for 16 weeks is capable of causing impairment of mitochondrial function with reduced expression of respiratory chain proteins in skeletal muscle. However, the RT was effective in preventing impairment of mitochondrial function in the skeletal muscle of rats exposed to secondary cigarette smoke. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-15 2023-07-29T14:13:21Z 2023-07-29T14:13:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.lfs.2022.121017 Life Sciences, v. 309. 1879-0631 0024-3205 http://hdl.handle.net/11449/249213 10.1016/j.lfs.2022.121017 2-s2.0-85139007107 |
url |
http://dx.doi.org/10.1016/j.lfs.2022.121017 http://hdl.handle.net/11449/249213 |
identifier_str_mv |
Life Sciences, v. 309. 1879-0631 0024-3205 10.1016/j.lfs.2022.121017 2-s2.0-85139007107 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Life Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129059026108416 |