Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.molcatb.2017.04.002 http://hdl.handle.net/11449/174462 |
Resumo: | In order to improve economic viability of an enzymatic process, the use of an operationally stable and low-cost biocatalyst is encouraged. Although the immobilization of lipases is widely reported, the search for new supports and immobilization protocols with better properties is still important. In this study, mono- and heterofunctionalized silica magnetic microparticles (SMMPs) were synthetized for immobilization of lipase B from Candida antarctica (CALB). The SMMPs were prepared in a micro-emulsion system containing sodium silicate and superparamagnetic iron oxide nanoparticles, followed by chemical modification with octyl groups and octyl plus aldehyde groups. These supports allowed the immobilization of CALB by hydrophobic adsorption or hydrophobic/covalent linkages, achieving immobilization yield of 88% and recovered activities of 128% and 59%, respectively. The performance of the magnetic biocatalysts was evaluated in the synthesis of xylose fatty acid esters (laurate or oleate) in tert-butyl alcohol medium, yielding around 60% conversion after 48 h under optimized conditions (xylose/fatty acid molar ratio of 1:0.2, 55 °C, and activity load of 37.5 U/g). The magnetic biocatalyst was used in 10 reaction cycles of 48 h at 46 °C maintaining high xylose conversions. Besides, the biocatalyst might be easily and quickly recovered from the reaction medium by an external magnetic field, an operational advantage in the case of viscous and complex media, e.g., medium containing insoluble sugars and molecular sieves. |
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Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipasesCandida antarctica lipase BHeterofunctionalizationSilica magnetic microparticlesXylose fatty acid esterIn order to improve economic viability of an enzymatic process, the use of an operationally stable and low-cost biocatalyst is encouraged. Although the immobilization of lipases is widely reported, the search for new supports and immobilization protocols with better properties is still important. In this study, mono- and heterofunctionalized silica magnetic microparticles (SMMPs) were synthetized for immobilization of lipase B from Candida antarctica (CALB). The SMMPs were prepared in a micro-emulsion system containing sodium silicate and superparamagnetic iron oxide nanoparticles, followed by chemical modification with octyl groups and octyl plus aldehyde groups. These supports allowed the immobilization of CALB by hydrophobic adsorption or hydrophobic/covalent linkages, achieving immobilization yield of 88% and recovered activities of 128% and 59%, respectively. The performance of the magnetic biocatalysts was evaluated in the synthesis of xylose fatty acid esters (laurate or oleate) in tert-butyl alcohol medium, yielding around 60% conversion after 48 h under optimized conditions (xylose/fatty acid molar ratio of 1:0.2, 55 °C, and activity load of 37.5 U/g). The magnetic biocatalyst was used in 10 reaction cycles of 48 h at 46 °C maintaining high xylose conversions. Besides, the biocatalyst might be easily and quickly recovered from the reaction medium by an external magnetic field, an operational advantage in the case of viscous and complex media, e.g., medium containing insoluble sugars and molecular sieves.Graduate Program in Chemical Engineering Department of Chemical Engineering Federal University of São Carlos, Rodovia Washington Luiz, km 235Department of Biochemistry and Chemical Technology Institute of Chemistry UNESP – São Paulo State UniversityKopp Technologies (KTech) Rua Alfredo Lopes, 1717, Jardim Macarengo, 13560-460Department of Biochemistry and Chemical Technology Institute of Chemistry UNESP – São Paulo State UniversityUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Rua Alfredo Lopesde Lima, Lionete NunesVieira, Gustavo Nakamura Alves [UNESP]Kopp, WillianTardioli, Paulo WaldirGiordano, Raquel L.C.2018-12-11T17:11:14Z2018-12-11T17:11:14Z2016-10-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleS491-S499application/pdfhttp://dx.doi.org/10.1016/j.molcatb.2017.04.002Journal of Molecular Catalysis B: Enzymatic, v. 133, p. S491-S499.1873-31581381-1177http://hdl.handle.net/11449/17446210.1016/j.molcatb.2017.04.0022-s2.0-850174354862-s2.0-85017435486.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Catalysis B: Enzymatic0,522info:eu-repo/semantics/openAccess2024-01-05T06:22:06Zoai:repositorio.unesp.br:11449/174462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:09:32.423132Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
spellingShingle |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases de Lima, Lionete Nunes Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester |
title_short |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_full |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_fullStr |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_full_unstemmed |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
title_sort |
Mono- and heterofunctionalized silica magnetic microparticles (SMMPs) as new carriers for immobilization of lipases |
author |
de Lima, Lionete Nunes |
author_facet |
de Lima, Lionete Nunes Vieira, Gustavo Nakamura Alves [UNESP] Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. |
author_role |
author |
author2 |
Vieira, Gustavo Nakamura Alves [UNESP] Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) Rua Alfredo Lopes |
dc.contributor.author.fl_str_mv |
de Lima, Lionete Nunes Vieira, Gustavo Nakamura Alves [UNESP] Kopp, Willian Tardioli, Paulo Waldir Giordano, Raquel L.C. |
dc.subject.por.fl_str_mv |
Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester |
topic |
Candida antarctica lipase B Heterofunctionalization Silica magnetic microparticles Xylose fatty acid ester |
description |
In order to improve economic viability of an enzymatic process, the use of an operationally stable and low-cost biocatalyst is encouraged. Although the immobilization of lipases is widely reported, the search for new supports and immobilization protocols with better properties is still important. In this study, mono- and heterofunctionalized silica magnetic microparticles (SMMPs) were synthetized for immobilization of lipase B from Candida antarctica (CALB). The SMMPs were prepared in a micro-emulsion system containing sodium silicate and superparamagnetic iron oxide nanoparticles, followed by chemical modification with octyl groups and octyl plus aldehyde groups. These supports allowed the immobilization of CALB by hydrophobic adsorption or hydrophobic/covalent linkages, achieving immobilization yield of 88% and recovered activities of 128% and 59%, respectively. The performance of the magnetic biocatalysts was evaluated in the synthesis of xylose fatty acid esters (laurate or oleate) in tert-butyl alcohol medium, yielding around 60% conversion after 48 h under optimized conditions (xylose/fatty acid molar ratio of 1:0.2, 55 °C, and activity load of 37.5 U/g). The magnetic biocatalyst was used in 10 reaction cycles of 48 h at 46 °C maintaining high xylose conversions. Besides, the biocatalyst might be easily and quickly recovered from the reaction medium by an external magnetic field, an operational advantage in the case of viscous and complex media, e.g., medium containing insoluble sugars and molecular sieves. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-29 2018-12-11T17:11:14Z 2018-12-11T17:11:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.molcatb.2017.04.002 Journal of Molecular Catalysis B: Enzymatic, v. 133, p. S491-S499. 1873-3158 1381-1177 http://hdl.handle.net/11449/174462 10.1016/j.molcatb.2017.04.002 2-s2.0-85017435486 2-s2.0-85017435486.pdf |
url |
http://dx.doi.org/10.1016/j.molcatb.2017.04.002 http://hdl.handle.net/11449/174462 |
identifier_str_mv |
Journal of Molecular Catalysis B: Enzymatic, v. 133, p. S491-S499. 1873-3158 1381-1177 10.1016/j.molcatb.2017.04.002 2-s2.0-85017435486 2-s2.0-85017435486.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Molecular Catalysis B: Enzymatic 0,522 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
S491-S499 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129398393536512 |