Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido

Detalhes bibliográficos
Autor(a) principal: Santana Nunes, Janaira
Data de Publicação: 2017
Outros Autores: Rocha de Brito, Manuela, Cunha Zied, Diego [UNESP], Aparecida das Graças Leite, Eloisa, Souza Dias, Eustáquio, Alves, Eduardo
Tipo de documento: Artigo
Idioma: eng
spa
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.riam.2016.04.006
http://hdl.handle.net/11449/178614
Resumo: Background Lecanicillium fungicola causes dry bubble disease in Agaricus bisporus mushrooms leading to significant economic losses in commercial production. Aims To monitor the infection process of L. fungicola in Brazilian strains of A. bisporus. Methods The interaction between the mycelium of L. fungicola (LF.1) and three strains of A. bisporus (ABI 7, ABI 11/14 and ABI 11/21) was studied. Electron microscopy and X-ray microanalyses of vegetative growth and basidiocarp infection were evaluated. Results Micrographs show that the vegetative mycelium of the Brazilian strains of A. bisporus is not infected by the parasite. The images show that the pathogen can interlace the hyphae of A. bisporus without causing damage, which contributes to the presence of L. fungicola during the substrate colonization, allowing their presence during primordial formation of A. bisporus. In the basidiocarp, germ tubes form within 16 h of infection with L. fungicola and the beginning of penetration takes place within 18 h, both without the formation of specialized structures. Conclusions Scanning electron microscopy enabled the process of colonization and reproduction to be observed within the formation of phialides, conidiophores and verticils of L. fungicola. The formation of calcium oxalate crystals by the pathogen was also visible using the X-ray microanalysis, both at the hyphae in the Petri plate and at basidiocarp infection site.
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spelling Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barridoEvaluation of the infection process by Lecanicillium fungicola in Agaricus bisporus by scanning electron microscopyMushroomMycopathogenScanning electron microscopyX-ray microanalysesBackground Lecanicillium fungicola causes dry bubble disease in Agaricus bisporus mushrooms leading to significant economic losses in commercial production. Aims To monitor the infection process of L. fungicola in Brazilian strains of A. bisporus. Methods The interaction between the mycelium of L. fungicola (LF.1) and three strains of A. bisporus (ABI 7, ABI 11/14 and ABI 11/21) was studied. Electron microscopy and X-ray microanalyses of vegetative growth and basidiocarp infection were evaluated. Results Micrographs show that the vegetative mycelium of the Brazilian strains of A. bisporus is not infected by the parasite. The images show that the pathogen can interlace the hyphae of A. bisporus without causing damage, which contributes to the presence of L. fungicola during the substrate colonization, allowing their presence during primordial formation of A. bisporus. In the basidiocarp, germ tubes form within 16 h of infection with L. fungicola and the beginning of penetration takes place within 18 h, both without the formation of specialized structures. Conclusions Scanning electron microscopy enabled the process of colonization and reproduction to be observed within the formation of phialides, conidiophores and verticils of L. fungicola. The formation of calcium oxalate crystals by the pathogen was also visible using the X-ray microanalysis, both at the hyphae in the Petri plate and at basidiocarp infection site.Universidade Federal de LavrasUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Tecnológicas Câmpus de DracenaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Tecnológicas Câmpus de DracenaUniversidade Federal de Lavras (UFLA)Universidade Estadual Paulista (Unesp)Santana Nunes, JanairaRocha de Brito, ManuelaCunha Zied, Diego [UNESP]Aparecida das Graças Leite, EloisaSouza Dias, EustáquioAlves, Eduardo2018-12-11T17:31:19Z2018-12-11T17:31:19Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article36-42application/pdfhttp://dx.doi.org/10.1016/j.riam.2016.04.006Revista Iberoamericana de Micologia, v. 34, n. 1, p. 36-42, 2017.2173-91881130-1406http://hdl.handle.net/11449/17861410.1016/j.riam.2016.04.0062-s2.0-850113713922-s2.0-85011371392.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengspaRevista Iberoamericana de Micologia0,432info:eu-repo/semantics/openAccess2024-05-07T13:47:37Zoai:repositorio.unesp.br:11449/178614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:27:36.162216Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
Evaluation of the infection process by Lecanicillium fungicola in Agaricus bisporus by scanning electron microscopy
title Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
spellingShingle Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
Santana Nunes, Janaira
Mushroom
Mycopathogen
Scanning electron microscopy
X-ray microanalyses
title_short Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
title_full Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
title_fullStr Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
title_full_unstemmed Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
title_sort Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
author Santana Nunes, Janaira
author_facet Santana Nunes, Janaira
Rocha de Brito, Manuela
Cunha Zied, Diego [UNESP]
Aparecida das Graças Leite, Eloisa
Souza Dias, Eustáquio
Alves, Eduardo
author_role author
author2 Rocha de Brito, Manuela
Cunha Zied, Diego [UNESP]
Aparecida das Graças Leite, Eloisa
Souza Dias, Eustáquio
Alves, Eduardo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Lavras (UFLA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Santana Nunes, Janaira
Rocha de Brito, Manuela
Cunha Zied, Diego [UNESP]
Aparecida das Graças Leite, Eloisa
Souza Dias, Eustáquio
Alves, Eduardo
dc.subject.por.fl_str_mv Mushroom
Mycopathogen
Scanning electron microscopy
X-ray microanalyses
topic Mushroom
Mycopathogen
Scanning electron microscopy
X-ray microanalyses
description Background Lecanicillium fungicola causes dry bubble disease in Agaricus bisporus mushrooms leading to significant economic losses in commercial production. Aims To monitor the infection process of L. fungicola in Brazilian strains of A. bisporus. Methods The interaction between the mycelium of L. fungicola (LF.1) and three strains of A. bisporus (ABI 7, ABI 11/14 and ABI 11/21) was studied. Electron microscopy and X-ray microanalyses of vegetative growth and basidiocarp infection were evaluated. Results Micrographs show that the vegetative mycelium of the Brazilian strains of A. bisporus is not infected by the parasite. The images show that the pathogen can interlace the hyphae of A. bisporus without causing damage, which contributes to the presence of L. fungicola during the substrate colonization, allowing their presence during primordial formation of A. bisporus. In the basidiocarp, germ tubes form within 16 h of infection with L. fungicola and the beginning of penetration takes place within 18 h, both without the formation of specialized structures. Conclusions Scanning electron microscopy enabled the process of colonization and reproduction to be observed within the formation of phialides, conidiophores and verticils of L. fungicola. The formation of calcium oxalate crystals by the pathogen was also visible using the X-ray microanalysis, both at the hyphae in the Petri plate and at basidiocarp infection site.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
2018-12-11T17:31:19Z
2018-12-11T17:31:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.riam.2016.04.006
Revista Iberoamericana de Micologia, v. 34, n. 1, p. 36-42, 2017.
2173-9188
1130-1406
http://hdl.handle.net/11449/178614
10.1016/j.riam.2016.04.006
2-s2.0-85011371392
2-s2.0-85011371392.pdf
url http://dx.doi.org/10.1016/j.riam.2016.04.006
http://hdl.handle.net/11449/178614
identifier_str_mv Revista Iberoamericana de Micologia, v. 34, n. 1, p. 36-42, 2017.
2173-9188
1130-1406
10.1016/j.riam.2016.04.006
2-s2.0-85011371392
2-s2.0-85011371392.pdf
dc.language.iso.fl_str_mv eng
spa
language eng
spa
dc.relation.none.fl_str_mv Revista Iberoamericana de Micologia
0,432
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 36-42
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129322072932352

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