Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | |
Tipo de documento: | Capítulo de livro |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/221089 |
Resumo: | Considering the main four steps for second-generation ethanol (E2G) production from a vegetal biomass, namely (1) pretreatment, (2) hydrolysis, (3) fermentation and (4) distillation, hydrolysis may be considered one of the most challenging operations. Although chemical or acidic hydrolysis is possible and somehow efficient, the enzymatic route of vegetal biomasses saccharification is prefered in most of the applications because it presents some advantages like mild conditions of operation, high specificity of the enymes and low generation of toxic residues, either for the environment or for the sucessive steps of the E2G production. However, mainly due to the restricted worldwide marketshare of enzymes, the cost of hydrolytic enzymes (such as cellulases, hemicellulases, ligninases and amylases) and of auxilar enzymes associated can make the enzymatic route of hydrolysis of vegetal biomasses unfeasible. In this context, the solidstate cultivation (SSC), especially of filamentous fungi on agro-industrial by-products (such as bagasses, brans, fruit pulp and peels and remaining on crops leaves) appears as a feasible and sustainable alternative for the supply of enzymes for E2G production chain. The main idea defended here is that the enzymes can be produced within the same biorefinery that have E2G as the major product, in which a paralel line can, at one end, be supplied with highly available vegetal biomasses as substrates for microbial cultivation and enzymes synthesis and, at the opposite end, supply the needed enzymes cocktails as an input for enzymatic hydrolysis or saccharification step on the central line of the biorefinery, the E2G production chain. On the above, in the current chapter, an overview of several works on enzymes production (cellulases, hemicellulases and amylases) by SSC using different types of bioreactors (packed-beds and rotating drums) is presented and discussed on the light of the available literature and on the findings of the research group with which the author has contributed since last decade. |
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Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternativeAgro-industrial by-productsEnzymatic hydrolysisSecond-generation ethanolSolid-state cultivationConsidering the main four steps for second-generation ethanol (E2G) production from a vegetal biomass, namely (1) pretreatment, (2) hydrolysis, (3) fermentation and (4) distillation, hydrolysis may be considered one of the most challenging operations. Although chemical or acidic hydrolysis is possible and somehow efficient, the enzymatic route of vegetal biomasses saccharification is prefered in most of the applications because it presents some advantages like mild conditions of operation, high specificity of the enymes and low generation of toxic residues, either for the environment or for the sucessive steps of the E2G production. However, mainly due to the restricted worldwide marketshare of enzymes, the cost of hydrolytic enzymes (such as cellulases, hemicellulases, ligninases and amylases) and of auxilar enzymes associated can make the enzymatic route of hydrolysis of vegetal biomasses unfeasible. In this context, the solidstate cultivation (SSC), especially of filamentous fungi on agro-industrial by-products (such as bagasses, brans, fruit pulp and peels and remaining on crops leaves) appears as a feasible and sustainable alternative for the supply of enzymes for E2G production chain. The main idea defended here is that the enzymes can be produced within the same biorefinery that have E2G as the major product, in which a paralel line can, at one end, be supplied with highly available vegetal biomasses as substrates for microbial cultivation and enzymes synthesis and, at the opposite end, supply the needed enzymes cocktails as an input for enzymatic hydrolysis or saccharification step on the central line of the biorefinery, the E2G production chain. On the above, in the current chapter, an overview of several works on enzymes production (cellulases, hemicellulases and amylases) by SSC using different types of bioreactors (packed-beds and rotating drums) is presented and discussed on the light of the available literature and on the findings of the research group with which the author has contributed since last decade.Department of Chemical Engineering Federal University of São CarlosDepartment of Chemistry and Environmental Sciences São Paulo State UniversityDepartment of Chemistry and Environmental Sciences São Paulo State UniversityUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Casciatori, Fernanda PerpétuaCasciatori-Frassatto, Priscila Aparecida [UNESP]2022-04-28T19:08:58Z2022-04-28T19:08:58Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart285-314Bioethanol and Beyond: Advances in Production Process and Future Directions, p. 285-314.http://hdl.handle.net/11449/2210892-s2.0-85048390506Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioethanol and Beyond: Advances in Production Process and Future Directionsinfo:eu-repo/semantics/openAccess2022-04-28T19:08:58Zoai:repositorio.unesp.br:11449/221089Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:08:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
title |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
spellingShingle |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative Casciatori, Fernanda Perpétua Agro-industrial by-products Enzymatic hydrolysis Second-generation ethanol Solid-state cultivation |
title_short |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
title_full |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
title_fullStr |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
title_full_unstemmed |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
title_sort |
Enzymes for the biochemical route of second-generation ethanol: Production by solid-state cultivation as a feasible and sustainable alternative |
author |
Casciatori, Fernanda Perpétua |
author_facet |
Casciatori, Fernanda Perpétua Casciatori-Frassatto, Priscila Aparecida [UNESP] |
author_role |
author |
author2 |
Casciatori-Frassatto, Priscila Aparecida [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Casciatori, Fernanda Perpétua Casciatori-Frassatto, Priscila Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Agro-industrial by-products Enzymatic hydrolysis Second-generation ethanol Solid-state cultivation |
topic |
Agro-industrial by-products Enzymatic hydrolysis Second-generation ethanol Solid-state cultivation |
description |
Considering the main four steps for second-generation ethanol (E2G) production from a vegetal biomass, namely (1) pretreatment, (2) hydrolysis, (3) fermentation and (4) distillation, hydrolysis may be considered one of the most challenging operations. Although chemical or acidic hydrolysis is possible and somehow efficient, the enzymatic route of vegetal biomasses saccharification is prefered in most of the applications because it presents some advantages like mild conditions of operation, high specificity of the enymes and low generation of toxic residues, either for the environment or for the sucessive steps of the E2G production. However, mainly due to the restricted worldwide marketshare of enzymes, the cost of hydrolytic enzymes (such as cellulases, hemicellulases, ligninases and amylases) and of auxilar enzymes associated can make the enzymatic route of hydrolysis of vegetal biomasses unfeasible. In this context, the solidstate cultivation (SSC), especially of filamentous fungi on agro-industrial by-products (such as bagasses, brans, fruit pulp and peels and remaining on crops leaves) appears as a feasible and sustainable alternative for the supply of enzymes for E2G production chain. The main idea defended here is that the enzymes can be produced within the same biorefinery that have E2G as the major product, in which a paralel line can, at one end, be supplied with highly available vegetal biomasses as substrates for microbial cultivation and enzymes synthesis and, at the opposite end, supply the needed enzymes cocktails as an input for enzymatic hydrolysis or saccharification step on the central line of the biorefinery, the E2G production chain. On the above, in the current chapter, an overview of several works on enzymes production (cellulases, hemicellulases and amylases) by SSC using different types of bioreactors (packed-beds and rotating drums) is presented and discussed on the light of the available literature and on the findings of the research group with which the author has contributed since last decade. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 2022-04-28T19:08:58Z 2022-04-28T19:08:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bookPart |
format |
bookPart |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Bioethanol and Beyond: Advances in Production Process and Future Directions, p. 285-314. http://hdl.handle.net/11449/221089 2-s2.0-85048390506 |
identifier_str_mv |
Bioethanol and Beyond: Advances in Production Process and Future Directions, p. 285-314. 2-s2.0-85048390506 |
url |
http://hdl.handle.net/11449/221089 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioethanol and Beyond: Advances in Production Process and Future Directions |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
285-314 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965437797072896 |