Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1519-6984.249617 http://hdl.handle.net/11449/218117 |
Resumo: | Hibernation is a natural condition of animals that lives in the temperate zone, although some tropical lizards also experience hibernation annually, such as the lizard native from South America, Salvator merianae, or “tegu” lizard. Even though physiological and metabolic characteristic associated with hibernation have been extensively studied, possible alterations in the red blood cells (RBC) integrity during this period remains unclear. Dehydration and fasting are natural consequences of hibernating for several months and it could be related to some cellular modifications. In this study, we investigated if the osmotic tolerance of RBCs of tegu lizard under hibernation is different from the cells obtained from animals while normal activity. Additionally, we indirectly investigated if the RBCs membrane of hibernating tegus could be associated with oxidation by quantifying oxidized biomolecules and the activity of antioxidant enzymes. Our findings suggest that RBCs are more fragile during the hibernation period, although we did not find evidence of an oxidative stress scenario associated with the accentuated fragility. Even though we did not exclude the possibility of oxidative damage during hibernation, we suggested that an increased RBCs volume as a consequence of hypoosmotic blood during hibernation could also affect RBCs integrity as noted. |
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Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernationEfeitos de oxidação e osmotolerância dos eritrócitos de Salvator merianae (Squamata: Teiidae) durante a hibernaçãoteguosmotic fragilityerythrocytesoxidative stresstegufragilidade osmóticaeritrócitosestresse oxidativoHibernation is a natural condition of animals that lives in the temperate zone, although some tropical lizards also experience hibernation annually, such as the lizard native from South America, Salvator merianae, or “tegu” lizard. Even though physiological and metabolic characteristic associated with hibernation have been extensively studied, possible alterations in the red blood cells (RBC) integrity during this period remains unclear. Dehydration and fasting are natural consequences of hibernating for several months and it could be related to some cellular modifications. In this study, we investigated if the osmotic tolerance of RBCs of tegu lizard under hibernation is different from the cells obtained from animals while normal activity. Additionally, we indirectly investigated if the RBCs membrane of hibernating tegus could be associated with oxidation by quantifying oxidized biomolecules and the activity of antioxidant enzymes. Our findings suggest that RBCs are more fragile during the hibernation period, although we did not find evidence of an oxidative stress scenario associated with the accentuated fragility. Even though we did not exclude the possibility of oxidative damage during hibernation, we suggested that an increased RBCs volume as a consequence of hypoosmotic blood during hibernation could also affect RBCs integrity as noted.A hibernação é uma condição natural dos animais que vivem na zona temperada, embora alguns lagartos tropicais também experenciem hibernação anualmente, como é o caso do lagarto nativo da América do Sul, Salvator merianae ou “teiú”. Embora as características fisiológicas e metabólicas associadas à hibernação tenham sido amplamente estudadas, possíveis alterações na integridade das hemácias durante esse período ainda permanecem obscuras. A desidratação e o jejum são consequências naturais da hibernação por vários meses e podem estar relacionadas a algumas modificações celulares. Neste estudo, investigamos se a tolerância osmótica de hemácias do lagarto teiú sob hibernação são diferentes das células obtidas de animais em atividade normal. Além disso, investigamos indiretamente por meio da quantificação de biomoléculas oxidadas e da atividade de enzimas antioxidantes se a membrana das hemácias dos teiús em hibernação poderia estar associada à oxidação. Nossos resultados sugerem que as hemácias possuem maior fragilidade durante o período de hibernação, embora não tenhamos encontrado evidências de um cenário de estresse oxidativo associado à essa fragilidade acentuada. Embora não tenhamos excluído a possibilidade de dano oxidativo durante a hibernação, sugerimos que um aumento no volume das hemácias como consequência de sangue hipoosmótico durante a hibernação também poderia afetar a integridade de hemácias, tal como foi observado.Universidade Estadual Paulista, Instituto de Biologia, Letras e Ciências ExatasUniversidade Federal de Mato Grosso do SulFundação Parque Tecnológico ItaipuUniversidade Estadual Paulista, Instituto de Biologia, Letras e Ciências ExatasInstituto Internacional de EcologiaUniversidade Estadual Paulista (UNESP)Universidade Federal de Mato Grosso do SulFundação Parque Tecnológico ItaipuVicente-ferreira, G. S. [UNESP]Martins, G. S. [UNESP]Chaves, N. A. [UNESP]Silva, D. G. H. [UNESP]Bonini-domingos, C. R. [UNESP]2022-04-28T16:55:57Z2022-04-28T16:55:57Z2021-10-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1519-6984.249617Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2021.1519-69841678-4375http://hdl.handle.net/11449/21811710.1590/1519-6984.249617S1519-69842024000100108S1519-69842024000100108.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2023-11-04T06:09:28Zoai:repositorio.unesp.br:11449/218117Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-04T06:09:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation Efeitos de oxidação e osmotolerância dos eritrócitos de Salvator merianae (Squamata: Teiidae) durante a hibernação |
title |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation |
spellingShingle |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation Vicente-ferreira, G. S. [UNESP] tegu osmotic fragility erythrocytes oxidative stress tegu fragilidade osmótica eritrócitos estresse oxidativo |
title_short |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation |
title_full |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation |
title_fullStr |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation |
title_full_unstemmed |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation |
title_sort |
Oxidative and osmotolerant effects in Salvator merianae (Squamata: Teiidae) red blood cells during hibernation |
author |
Vicente-ferreira, G. S. [UNESP] |
author_facet |
Vicente-ferreira, G. S. [UNESP] Martins, G. S. [UNESP] Chaves, N. A. [UNESP] Silva, D. G. H. [UNESP] Bonini-domingos, C. R. [UNESP] |
author_role |
author |
author2 |
Martins, G. S. [UNESP] Chaves, N. A. [UNESP] Silva, D. G. H. [UNESP] Bonini-domingos, C. R. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de Mato Grosso do Sul Fundação Parque Tecnológico Itaipu |
dc.contributor.author.fl_str_mv |
Vicente-ferreira, G. S. [UNESP] Martins, G. S. [UNESP] Chaves, N. A. [UNESP] Silva, D. G. H. [UNESP] Bonini-domingos, C. R. [UNESP] |
dc.subject.por.fl_str_mv |
tegu osmotic fragility erythrocytes oxidative stress tegu fragilidade osmótica eritrócitos estresse oxidativo |
topic |
tegu osmotic fragility erythrocytes oxidative stress tegu fragilidade osmótica eritrócitos estresse oxidativo |
description |
Hibernation is a natural condition of animals that lives in the temperate zone, although some tropical lizards also experience hibernation annually, such as the lizard native from South America, Salvator merianae, or “tegu” lizard. Even though physiological and metabolic characteristic associated with hibernation have been extensively studied, possible alterations in the red blood cells (RBC) integrity during this period remains unclear. Dehydration and fasting are natural consequences of hibernating for several months and it could be related to some cellular modifications. In this study, we investigated if the osmotic tolerance of RBCs of tegu lizard under hibernation is different from the cells obtained from animals while normal activity. Additionally, we indirectly investigated if the RBCs membrane of hibernating tegus could be associated with oxidation by quantifying oxidized biomolecules and the activity of antioxidant enzymes. Our findings suggest that RBCs are more fragile during the hibernation period, although we did not find evidence of an oxidative stress scenario associated with the accentuated fragility. Even though we did not exclude the possibility of oxidative damage during hibernation, we suggested that an increased RBCs volume as a consequence of hypoosmotic blood during hibernation could also affect RBCs integrity as noted. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-29 2022-04-28T16:55:57Z 2022-04-28T16:55:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1519-6984.249617 Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2021. 1519-6984 1678-4375 http://hdl.handle.net/11449/218117 10.1590/1519-6984.249617 S1519-69842024000100108 S1519-69842024000100108.pdf |
url |
http://dx.doi.org/10.1590/1519-6984.249617 http://hdl.handle.net/11449/218117 |
identifier_str_mv |
Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2021. 1519-6984 1678-4375 10.1590/1519-6984.249617 S1519-69842024000100108 S1519-69842024000100108.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046314886299648 |