Blood cells attachment after root conditioning and PRP application: An in vitro study

• Autor(a) principal: Dantas, Andréa Abi Rached [UNESP]
• Data de Publicação: 2012
• Outros Autores: Fontanari, Lucas Amaral [UNESP], de Paula Ishi, Eduardo, Leite, Fábio Renato Manzolli, Zandim-Barcelos, Daniela Leal [UNESP], Rached, Ricardo Samih Georges Abi [UNESP], Sampaio, José Eduardo Cezar [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.5005/jp-journals-10024-1147; http://hdl.handle.net/11449/73311
• topic: Blood; Citric acid; Edetic acid; Laboratory research; Platelet-rich plasma; Root conditioning; Smear layer; Tetracycline; chelating agent; citrate sodium; citric acid; edetic acid; fibrin; tetracycline; blood cell; blood clotting; cell adhesion; cementum; dental surgery; dentin; drug effect; human; male; physiology; reparative dentistry; scanning electron microscopy; thrombocyte rich plasma; tooth disease; tooth root; ultrastructure; Blood Cells; Blood Coagulation; Cell Adhesion; Chelating Agents; Citrates; Citric Acid; Dental Cementum; Dentin; Edetic Acid; Fibrin; Humans; Male; Microscopy, Electron, Scanning; Platelet-Rich Plasma; Smear Layer; Tissue Conditioning (Dental); Tooth Demineralization; Tooth Root

Aim: Root conditioning is aimed at smear layer removal and at dental matrix collagen exposure, which may promote periodontal regeneration. This in vitro study assessed smear layer removal, collagen fiber exposure and the influence of PRP (platelet-rich plasma) application on adhesion of blood cells to the root surface using scanning electron microscopy (SEM). Materials and methods: Scaled root samples (n = 160) were set in five groups and conditioned with: group I - control group (saline solution); group II (EDTA 24%); group III (citric acid 25%); group IV (tetracycline hydrochloride 50 mg/ml); group V (sodium citrate 30%). Eighty samples were assessed using the root surface modification index (RSMI). The other eighty samples were set in two groups. The first group (n = 40) received PRP gel application with a soft brush and the second group (n = 40) received PRP application and then a blood drop. The fibrin clot formation was assessed in the first group and the blood cells adhesion was assessed in the second group using the BEAI (blood elements adhesion index). A previously trained, calibrated, and blind examiner evaluated photomicrographs. Statistical analysis was performed using the Kruskal-Wallis's and Dunn's tests. Results: Group III attained the best results for RSMI and BEAI. Moreover, it was the only group showing fibrin clot formation. Conclusion: Citric acid was the most efficient conditioner for smear layer removal, collagen fiber exposure and blood cell adhesion. Moreover, it was the only group showing fibrin clot formation after PRP application. Clinical significance: This study demonstrated that root conditioning followed by PRP application may favor blood cell adhesion on root surface which may optimize periodontal healing.