Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)

• Autor(a) principal: Petacci, Fernando
• Data de Publicação: 2010
• Outros Autores: Freitas, Silvia S., Brunetti, Iguatemy Lourenço [UNESP], Khalil, Najeh M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4067/S0716-97602010000100008; http://hdl.handle.net/11449/7244
• topic: Astilbin; Myeloperoxidase; Horseradish peroxidase; Reactive Oxygen Species; Hypochlorous acid

Astilbin (5,7,3',4'-tetrahydroxy-2,3-dihydroflavonol-3-beta-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra molls, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and horseradish peroxidase (HRP), and its antioxidant activity against hypochlorous acid (HOCl) and total antioxidant activity (TAC) by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS center dot+). Astilbin inhibited MPO and HRP activities in a concentration-dependent relationship and effectively scavenged HOC. The TAC by ABTS center dot+ of astilbin (IC50 similar to 20 mM) was higher than that of uric acid, which was used as a positive control. These data demonstrate that astilbin is a potent antioxidant and that it inhibits MPO and HRP activities efficiently.