Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/cbin.11458 http://hdl.handle.net/11449/201008 |
Resumo: | The postlactational involution of the mammary gland is a complex process. It involves the collapse of the alveoli and the remodeling of the extracellular matrix, which in turn implies a complex set of interrelations between the epithelial, stromal, and extracellular matrix elements. The telocytes, a new type of CD34-positive stromal cell that differs from fibroblasts in morphological terms and gene expression, were detected in the stroma of several tissues, including the mammary gland; however, their function remains elusive. The present study employed three-dimensional reconstructions and immunohistochemical, ultrastructural, and immunofluorescence techniques in histological sections of the mammary gland of the Mongolian gerbil during lactation and postlactational involution to evaluate the presence of telocytes and to investigate a possible function for these cells. By means of immunofluorescence assays for CD34 and c-kit, major markers of telocytes, and also through morphological and ultrastructural evidences, telocytes were observed to surround the mammary ducts and collapsing alveoli. It was also found that these cells are associated with matrix metalloproteinase 9, which indicates that telocytes can play a role in extracellular matrix digestion, as well as vascular endothelial growth factor, a factor that promotes angiogenesis. Together, these data indicate that telocytes are a distinct cell type in the mammary gland and, for the first time, show that these cells possibly play a role in tissue remodeling and angiogenesis during the postlactional involution of the mammary gland. |
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Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbilsCD34extracellular matrix remodelinggerbilmammary gland involutiontelocytesTGF-β1VEGFThe postlactational involution of the mammary gland is a complex process. It involves the collapse of the alveoli and the remodeling of the extracellular matrix, which in turn implies a complex set of interrelations between the epithelial, stromal, and extracellular matrix elements. The telocytes, a new type of CD34-positive stromal cell that differs from fibroblasts in morphological terms and gene expression, were detected in the stroma of several tissues, including the mammary gland; however, their function remains elusive. The present study employed three-dimensional reconstructions and immunohistochemical, ultrastructural, and immunofluorescence techniques in histological sections of the mammary gland of the Mongolian gerbil during lactation and postlactational involution to evaluate the presence of telocytes and to investigate a possible function for these cells. By means of immunofluorescence assays for CD34 and c-kit, major markers of telocytes, and also through morphological and ultrastructural evidences, telocytes were observed to surround the mammary ducts and collapsing alveoli. It was also found that these cells are associated with matrix metalloproteinase 9, which indicates that telocytes can play a role in extracellular matrix digestion, as well as vascular endothelial growth factor, a factor that promotes angiogenesis. Together, these data indicate that telocytes are a distinct cell type in the mammary gland and, for the first time, show that these cells possibly play a role in tissue remodeling and angiogenesis during the postlactional involution of the mammary gland.Department of Structural and Functional Biology State University of CampinasLaboratory of Microscopy and Microanalysis, Department of Biology University of Estadual Paulista (UNESP)Department of Structural and Functional Biology Institute of Biosciences University of Estadual Paulista (UNESP)Laboratory of Microscopy and Microanalysis, Department of Biology University of Estadual Paulista (UNESP)Department of Structural and Functional Biology Institute of Biosciences University of Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Sanches, Bruno D. A.Leonel, Ellen C. R. [UNESP]Maldarine, Juliana S.Tamarindo, Guilherme H.Barquilha, Caroline N. [UNESP]Felisbino, Sérgio L. [UNESP]Goés, Rejane M. [UNESP]Vilamaior, Patricia S. L. [UNESP]Taboga, Sebastião R. [UNESP]2020-12-12T02:21:44Z2020-12-12T02:21:44Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/cbin.11458Cell Biology International.1095-83551065-6995http://hdl.handle.net/11449/20100810.1002/cbin.114582-s2.0-85090312721Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCell Biology Internationalinfo:eu-repo/semantics/openAccess2024-04-11T19:33:51Zoai:repositorio.unesp.br:11449/201008Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:37:52.944263Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
title |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
spellingShingle |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils Sanches, Bruno D. A. CD34 extracellular matrix remodeling gerbil mammary gland involution telocytes TGF-β1 VEGF |
title_short |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
title_full |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
title_fullStr |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
title_full_unstemmed |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
title_sort |
Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils |
author |
Sanches, Bruno D. A. |
author_facet |
Sanches, Bruno D. A. Leonel, Ellen C. R. [UNESP] Maldarine, Juliana S. Tamarindo, Guilherme H. Barquilha, Caroline N. [UNESP] Felisbino, Sérgio L. [UNESP] Goés, Rejane M. [UNESP] Vilamaior, Patricia S. L. [UNESP] Taboga, Sebastião R. [UNESP] |
author_role |
author |
author2 |
Leonel, Ellen C. R. [UNESP] Maldarine, Juliana S. Tamarindo, Guilherme H. Barquilha, Caroline N. [UNESP] Felisbino, Sérgio L. [UNESP] Goés, Rejane M. [UNESP] Vilamaior, Patricia S. L. [UNESP] Taboga, Sebastião R. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sanches, Bruno D. A. Leonel, Ellen C. R. [UNESP] Maldarine, Juliana S. Tamarindo, Guilherme H. Barquilha, Caroline N. [UNESP] Felisbino, Sérgio L. [UNESP] Goés, Rejane M. [UNESP] Vilamaior, Patricia S. L. [UNESP] Taboga, Sebastião R. [UNESP] |
dc.subject.por.fl_str_mv |
CD34 extracellular matrix remodeling gerbil mammary gland involution telocytes TGF-β1 VEGF |
topic |
CD34 extracellular matrix remodeling gerbil mammary gland involution telocytes TGF-β1 VEGF |
description |
The postlactational involution of the mammary gland is a complex process. It involves the collapse of the alveoli and the remodeling of the extracellular matrix, which in turn implies a complex set of interrelations between the epithelial, stromal, and extracellular matrix elements. The telocytes, a new type of CD34-positive stromal cell that differs from fibroblasts in morphological terms and gene expression, were detected in the stroma of several tissues, including the mammary gland; however, their function remains elusive. The present study employed three-dimensional reconstructions and immunohistochemical, ultrastructural, and immunofluorescence techniques in histological sections of the mammary gland of the Mongolian gerbil during lactation and postlactational involution to evaluate the presence of telocytes and to investigate a possible function for these cells. By means of immunofluorescence assays for CD34 and c-kit, major markers of telocytes, and also through morphological and ultrastructural evidences, telocytes were observed to surround the mammary ducts and collapsing alveoli. It was also found that these cells are associated with matrix metalloproteinase 9, which indicates that telocytes can play a role in extracellular matrix digestion, as well as vascular endothelial growth factor, a factor that promotes angiogenesis. Together, these data indicate that telocytes are a distinct cell type in the mammary gland and, for the first time, show that these cells possibly play a role in tissue remodeling and angiogenesis during the postlactional involution of the mammary gland. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:21:44Z 2020-12-12T02:21:44Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/cbin.11458 Cell Biology International. 1095-8355 1065-6995 http://hdl.handle.net/11449/201008 10.1002/cbin.11458 2-s2.0-85090312721 |
url |
http://dx.doi.org/10.1002/cbin.11458 http://hdl.handle.net/11449/201008 |
identifier_str_mv |
Cell Biology International. 1095-8355 1065-6995 10.1002/cbin.11458 2-s2.0-85090312721 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cell Biology International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129098307862528 |