Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-016-2140-3 http://hdl.handle.net/11449/159495 |
Resumo: | For better evaluation of the efficacy of low-level laser therapy in treating painful diabetic neuropathy and in protecting nerve fiber damage, we conducted a study with type 1 diabetic rats induced by streptozotocin. It is well known that diabetic peripheral neuropathy is the leading cause of pain in those individuals who suffer from diabetes. Despite the efficacy of insulin in controlling glucose level in blood, there is no effective treatment to prevent or reverse neuropathic damage for total pain relief.Male Wistar rats were divided into saline, vehicle, and treatment groups. A single intraperitoneal (i.p.) injection of streptozotocin (STZ) (85 mg/kg) was administered for the induction of diabetes. The von Frey filaments were used to assess nociceptive thresholds (allodynia). Behavioral measurements were accessed 14, 28, 48, and 56 days after STZ administration. Rats were irradiated with GaAs Laser (Gallium Arsenide, Laserpulse, Ibramed Brazil) emitting a wavelength of 904 nm, an output power of 45 mWpk, beam spot size at target 0.13 cm(2), a frequency of 9500 Hz, a pulse time 60 ns, and an energy density of 6,23 J/cm(2).The application of four sessions of low-level laser therapy was sufficient to reverse allodynia and protect peripheral nerve damage in diabetic rats.The results of this study indicate that low-level laser therapy is feasible to treat painful diabetic condition in rats using this protocol. Although its efficacy in reversing painful stimuli and protecting nerve fibers from damage was demonstrated, this treatment protocol must be further evaluated in biochemical levels to confirm its biological effects. |
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Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetesDiabetes mellitusSciatic nerveNociceptionMyelin sheathRatStreptozotocinFor better evaluation of the efficacy of low-level laser therapy in treating painful diabetic neuropathy and in protecting nerve fiber damage, we conducted a study with type 1 diabetic rats induced by streptozotocin. It is well known that diabetic peripheral neuropathy is the leading cause of pain in those individuals who suffer from diabetes. Despite the efficacy of insulin in controlling glucose level in blood, there is no effective treatment to prevent or reverse neuropathic damage for total pain relief.Male Wistar rats were divided into saline, vehicle, and treatment groups. A single intraperitoneal (i.p.) injection of streptozotocin (STZ) (85 mg/kg) was administered for the induction of diabetes. The von Frey filaments were used to assess nociceptive thresholds (allodynia). Behavioral measurements were accessed 14, 28, 48, and 56 days after STZ administration. Rats were irradiated with GaAs Laser (Gallium Arsenide, Laserpulse, Ibramed Brazil) emitting a wavelength of 904 nm, an output power of 45 mWpk, beam spot size at target 0.13 cm(2), a frequency of 9500 Hz, a pulse time 60 ns, and an energy density of 6,23 J/cm(2).The application of four sessions of low-level laser therapy was sufficient to reverse allodynia and protect peripheral nerve damage in diabetic rats.The results of this study indicate that low-level laser therapy is feasible to treat painful diabetic condition in rats using this protocol. Although its efficacy in reversing painful stimuli and protecting nerve fibers from damage was demonstrated, this treatment protocol must be further evaluated in biochemical levels to confirm its biological effects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Ave Prof Lineu Prestes 2415, BR-05508900 Sao Paulo, BrazilUniv Estadual Paulista, Inst Biosci, BR-13506900 Sao Paulo, BrazilUniv Estadual Paulista, Inst Biosci, BR-13506900 Sao Paulo, BrazilFAPESP: 2014/24533-0SpringerUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Correia Rocha, Igor RafaelCiena, Adriano Polican [UNESP]Rosa, Alyne SantanaMartins, Daniel OliveiraChacur, Marucia2018-11-26T15:44:01Z2018-11-26T15:44:01Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article495-501application/pdfhttp://dx.doi.org/10.1007/s10103-016-2140-3Lasers In Medical Science. London: Springer London Ltd, v. 32, n. 3, p. 495-501, 2017.0268-8921http://hdl.handle.net/11449/15949510.1007/s10103-016-2140-3WOS:000398716200002WOS000398716200002.pdf3940359723253380000-0002-2912-3376Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers In Medical Science0,713info:eu-repo/semantics/openAccess2024-01-08T06:26:19Zoai:repositorio.unesp.br:11449/159495Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:26:29.968761Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
title |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
spellingShingle |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes Correia Rocha, Igor Rafael Diabetes mellitus Sciatic nerve Nociception Myelin sheath Rat Streptozotocin |
title_short |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
title_full |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
title_fullStr |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
title_full_unstemmed |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
title_sort |
Photobiostimulation reverses allodynia and peripheral nerve damage in streptozotocin-induced type 1 diabetes |
author |
Correia Rocha, Igor Rafael |
author_facet |
Correia Rocha, Igor Rafael Ciena, Adriano Polican [UNESP] Rosa, Alyne Santana Martins, Daniel Oliveira Chacur, Marucia |
author_role |
author |
author2 |
Ciena, Adriano Polican [UNESP] Rosa, Alyne Santana Martins, Daniel Oliveira Chacur, Marucia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Correia Rocha, Igor Rafael Ciena, Adriano Polican [UNESP] Rosa, Alyne Santana Martins, Daniel Oliveira Chacur, Marucia |
dc.subject.por.fl_str_mv |
Diabetes mellitus Sciatic nerve Nociception Myelin sheath Rat Streptozotocin |
topic |
Diabetes mellitus Sciatic nerve Nociception Myelin sheath Rat Streptozotocin |
description |
For better evaluation of the efficacy of low-level laser therapy in treating painful diabetic neuropathy and in protecting nerve fiber damage, we conducted a study with type 1 diabetic rats induced by streptozotocin. It is well known that diabetic peripheral neuropathy is the leading cause of pain in those individuals who suffer from diabetes. Despite the efficacy of insulin in controlling glucose level in blood, there is no effective treatment to prevent or reverse neuropathic damage for total pain relief.Male Wistar rats were divided into saline, vehicle, and treatment groups. A single intraperitoneal (i.p.) injection of streptozotocin (STZ) (85 mg/kg) was administered for the induction of diabetes. The von Frey filaments were used to assess nociceptive thresholds (allodynia). Behavioral measurements were accessed 14, 28, 48, and 56 days after STZ administration. Rats were irradiated with GaAs Laser (Gallium Arsenide, Laserpulse, Ibramed Brazil) emitting a wavelength of 904 nm, an output power of 45 mWpk, beam spot size at target 0.13 cm(2), a frequency of 9500 Hz, a pulse time 60 ns, and an energy density of 6,23 J/cm(2).The application of four sessions of low-level laser therapy was sufficient to reverse allodynia and protect peripheral nerve damage in diabetic rats.The results of this study indicate that low-level laser therapy is feasible to treat painful diabetic condition in rats using this protocol. Although its efficacy in reversing painful stimuli and protecting nerve fibers from damage was demonstrated, this treatment protocol must be further evaluated in biochemical levels to confirm its biological effects. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-11-26T15:44:01Z 2018-11-26T15:44:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-016-2140-3 Lasers In Medical Science. London: Springer London Ltd, v. 32, n. 3, p. 495-501, 2017. 0268-8921 http://hdl.handle.net/11449/159495 10.1007/s10103-016-2140-3 WOS:000398716200002 WOS000398716200002.pdf 394035972325338 0000-0002-2912-3376 |
url |
http://dx.doi.org/10.1007/s10103-016-2140-3 http://hdl.handle.net/11449/159495 |
identifier_str_mv |
Lasers In Medical Science. London: Springer London Ltd, v. 32, n. 3, p. 495-501, 2017. 0268-8921 10.1007/s10103-016-2140-3 WOS:000398716200002 WOS000398716200002.pdf 394035972325338 0000-0002-2912-3376 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers In Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
495-501 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129427855376384 |