Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes

Detalhes bibliográficos
Autor(a) principal: Schmidt, Elizabeth Moreira dos Santos [UNESP]
Data de Publicação: 2021
Outros Autores: Fachiolli, Daniele Floriano [UNESP], de Oliveira, Raphaela Moreira [UNESP], Almeida, Fabiana Alves [UNESP], Pariz, Cristiano Magalhães [UNESP], de Lima Meirelles, Paulo Roberto [UNESP], Costa, Ciniro [UNESP], Tvarijonaviciute, Asta, Erel, Ozcan, Neselioglu, Salim, Ceron, Jose Joaquin, Rubio, Camila Peres [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.3390/ani11102856
Texto Completo: http://dx.doi.org/10.3390/ani11102856
http://hdl.handle.net/11449/229616
Resumo: This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.
id UNSP_a73f094c728ba8daaba391cb3b30feed
oai_identifier_str oai:repositorio.unesp.br:11449/229616
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodesAntioxidantClosantelHaemonchusIntegrated crop-livestockOxidative stressRuminantsThis work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Faculty of Veterinary Medicine University Center of the Federal District-UDFDepartment of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Department of Parasitology Biosciences Institute Campus of Botucatu São Paulo State University (FMVZ-UNESP)Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU) Regional Campus of International Excellence ‘Campus Mare Nostrum’ University of Murcia, EspinardoDepartment of Biochemistry Faculty of Medicine Ankara Yildirim Beyazit University Ankara City HospitalDepartment of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Department of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Department of Parasitology Biosciences Institute Campus of Botucatu São Paulo State University (FMVZ-UNESP)FAPESP: 2017/05055Universidade Estadual Paulista (UNESP)University Center of the Federal District-UDFUniversity of MurciaAnkara City HospitalSchmidt, Elizabeth Moreira dos Santos [UNESP]Fachiolli, Daniele Floriano [UNESP]de Oliveira, Raphaela Moreira [UNESP]Almeida, Fabiana Alves [UNESP]Pariz, Cristiano Magalhães [UNESP]de Lima Meirelles, Paulo Roberto [UNESP]Costa, Ciniro [UNESP]Tvarijonaviciute, AstaErel, OzcanNeselioglu, SalimCeron, Jose JoaquinRubio, Camila Peres [UNESP]2022-04-29T08:34:50Z2022-04-29T08:34:50Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani11102856Animals, v. 11, n. 10, 2021.2076-2615http://hdl.handle.net/11449/22961610.3390/ani111028562-s2.0-85116015795Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2024-09-06T18:55:10Zoai:repositorio.unesp.br:11449/229616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-06T18:55:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
title Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
spellingShingle Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
Schmidt, Elizabeth Moreira dos Santos [UNESP]
Antioxidant
Closantel
Haemonchus
Integrated crop-livestock
Oxidative stress
Ruminants
Schmidt, Elizabeth Moreira dos Santos [UNESP]
Antioxidant
Closantel
Haemonchus
Integrated crop-livestock
Oxidative stress
Ruminants
title_short Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
title_full Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
title_fullStr Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
title_full_unstemmed Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
title_sort Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
author Schmidt, Elizabeth Moreira dos Santos [UNESP]
author_facet Schmidt, Elizabeth Moreira dos Santos [UNESP]
Schmidt, Elizabeth Moreira dos Santos [UNESP]
Fachiolli, Daniele Floriano [UNESP]
de Oliveira, Raphaela Moreira [UNESP]
Almeida, Fabiana Alves [UNESP]
Pariz, Cristiano Magalhães [UNESP]
de Lima Meirelles, Paulo Roberto [UNESP]
Costa, Ciniro [UNESP]
Tvarijonaviciute, Asta
Erel, Ozcan
Neselioglu, Salim
Ceron, Jose Joaquin
Rubio, Camila Peres [UNESP]
Fachiolli, Daniele Floriano [UNESP]
de Oliveira, Raphaela Moreira [UNESP]
Almeida, Fabiana Alves [UNESP]
Pariz, Cristiano Magalhães [UNESP]
de Lima Meirelles, Paulo Roberto [UNESP]
Costa, Ciniro [UNESP]
Tvarijonaviciute, Asta
Erel, Ozcan
Neselioglu, Salim
Ceron, Jose Joaquin
Rubio, Camila Peres [UNESP]
author_role author
author2 Fachiolli, Daniele Floriano [UNESP]
de Oliveira, Raphaela Moreira [UNESP]
Almeida, Fabiana Alves [UNESP]
Pariz, Cristiano Magalhães [UNESP]
de Lima Meirelles, Paulo Roberto [UNESP]
Costa, Ciniro [UNESP]
Tvarijonaviciute, Asta
Erel, Ozcan
Neselioglu, Salim
Ceron, Jose Joaquin
Rubio, Camila Peres [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University Center of the Federal District-UDF
University of Murcia
Ankara City Hospital
dc.contributor.author.fl_str_mv Schmidt, Elizabeth Moreira dos Santos [UNESP]
Fachiolli, Daniele Floriano [UNESP]
de Oliveira, Raphaela Moreira [UNESP]
Almeida, Fabiana Alves [UNESP]
Pariz, Cristiano Magalhães [UNESP]
de Lima Meirelles, Paulo Roberto [UNESP]
Costa, Ciniro [UNESP]
Tvarijonaviciute, Asta
Erel, Ozcan
Neselioglu, Salim
Ceron, Jose Joaquin
Rubio, Camila Peres [UNESP]
dc.subject.por.fl_str_mv Antioxidant
Closantel
Haemonchus
Integrated crop-livestock
Oxidative stress
Ruminants
topic Antioxidant
Closantel
Haemonchus
Integrated crop-livestock
Oxidative stress
Ruminants
description This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-01
2022-04-29T08:34:50Z
2022-04-29T08:34:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ani11102856
Animals, v. 11, n. 10, 2021.
2076-2615
http://hdl.handle.net/11449/229616
10.3390/ani11102856
2-s2.0-85116015795
url http://dx.doi.org/10.3390/ani11102856
http://hdl.handle.net/11449/229616
identifier_str_mv Animals, v. 11, n. 10, 2021.
2076-2615
10.3390/ani11102856
2-s2.0-85116015795
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animals
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1822183855818276864
dc.identifier.doi.none.fl_str_mv 10.3390/ani11102856