Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.3390/ani11102856 |
Texto Completo: | http://dx.doi.org/10.3390/ani11102856 http://hdl.handle.net/11449/229616 |
Resumo: | This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease. |
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Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodesAntioxidantClosantelHaemonchusIntegrated crop-livestockOxidative stressRuminantsThis work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Faculty of Veterinary Medicine University Center of the Federal District-UDFDepartment of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Department of Parasitology Biosciences Institute Campus of Botucatu São Paulo State University (FMVZ-UNESP)Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU) Regional Campus of International Excellence ‘Campus Mare Nostrum’ University of Murcia, EspinardoDepartment of Biochemistry Faculty of Medicine Ankara Yildirim Beyazit University Ankara City HospitalDepartment of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Department of Animal Nutrition and Breeding School of Veterinary Medicine and Animal Science Campus of Botucatu São Paulo State University (FMVZ-UNESP)Department of Parasitology Biosciences Institute Campus of Botucatu São Paulo State University (FMVZ-UNESP)FAPESP: 2017/05055Universidade Estadual Paulista (UNESP)University Center of the Federal District-UDFUniversity of MurciaAnkara City HospitalSchmidt, Elizabeth Moreira dos Santos [UNESP]Fachiolli, Daniele Floriano [UNESP]de Oliveira, Raphaela Moreira [UNESP]Almeida, Fabiana Alves [UNESP]Pariz, Cristiano Magalhães [UNESP]de Lima Meirelles, Paulo Roberto [UNESP]Costa, Ciniro [UNESP]Tvarijonaviciute, AstaErel, OzcanNeselioglu, SalimCeron, Jose JoaquinRubio, Camila Peres [UNESP]2022-04-29T08:34:50Z2022-04-29T08:34:50Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani11102856Animals, v. 11, n. 10, 2021.2076-2615http://hdl.handle.net/11449/22961610.3390/ani111028562-s2.0-85116015795Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2024-09-06T18:55:10Zoai:repositorio.unesp.br:11449/229616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-06T18:55:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
title |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
spellingShingle |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes Schmidt, Elizabeth Moreira dos Santos [UNESP] Antioxidant Closantel Haemonchus Integrated crop-livestock Oxidative stress Ruminants Schmidt, Elizabeth Moreira dos Santos [UNESP] Antioxidant Closantel Haemonchus Integrated crop-livestock Oxidative stress Ruminants |
title_short |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
title_full |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
title_fullStr |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
title_full_unstemmed |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
title_sort |
Changes in serum thiol-disulphide homeostasis in sheep with gastrointestinal nematodes |
author |
Schmidt, Elizabeth Moreira dos Santos [UNESP] |
author_facet |
Schmidt, Elizabeth Moreira dos Santos [UNESP] Schmidt, Elizabeth Moreira dos Santos [UNESP] Fachiolli, Daniele Floriano [UNESP] de Oliveira, Raphaela Moreira [UNESP] Almeida, Fabiana Alves [UNESP] Pariz, Cristiano Magalhães [UNESP] de Lima Meirelles, Paulo Roberto [UNESP] Costa, Ciniro [UNESP] Tvarijonaviciute, Asta Erel, Ozcan Neselioglu, Salim Ceron, Jose Joaquin Rubio, Camila Peres [UNESP] Fachiolli, Daniele Floriano [UNESP] de Oliveira, Raphaela Moreira [UNESP] Almeida, Fabiana Alves [UNESP] Pariz, Cristiano Magalhães [UNESP] de Lima Meirelles, Paulo Roberto [UNESP] Costa, Ciniro [UNESP] Tvarijonaviciute, Asta Erel, Ozcan Neselioglu, Salim Ceron, Jose Joaquin Rubio, Camila Peres [UNESP] |
author_role |
author |
author2 |
Fachiolli, Daniele Floriano [UNESP] de Oliveira, Raphaela Moreira [UNESP] Almeida, Fabiana Alves [UNESP] Pariz, Cristiano Magalhães [UNESP] de Lima Meirelles, Paulo Roberto [UNESP] Costa, Ciniro [UNESP] Tvarijonaviciute, Asta Erel, Ozcan Neselioglu, Salim Ceron, Jose Joaquin Rubio, Camila Peres [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University Center of the Federal District-UDF University of Murcia Ankara City Hospital |
dc.contributor.author.fl_str_mv |
Schmidt, Elizabeth Moreira dos Santos [UNESP] Fachiolli, Daniele Floriano [UNESP] de Oliveira, Raphaela Moreira [UNESP] Almeida, Fabiana Alves [UNESP] Pariz, Cristiano Magalhães [UNESP] de Lima Meirelles, Paulo Roberto [UNESP] Costa, Ciniro [UNESP] Tvarijonaviciute, Asta Erel, Ozcan Neselioglu, Salim Ceron, Jose Joaquin Rubio, Camila Peres [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant Closantel Haemonchus Integrated crop-livestock Oxidative stress Ruminants |
topic |
Antioxidant Closantel Haemonchus Integrated crop-livestock Oxidative stress Ruminants |
description |
This work aimed to evaluate the thiol-disulphide homeostasis in serum of lambs naturally infected by gastrointestinal nematodes presenting different levels of parasite load indirectly indicated by faecal worm egg counts (EPG). Furthermore, the possible changes in the thiol-disulphide dynamic after different procedures to reduce the parasitic charge, such as the integrated crop-livestock system or anthelmintic treatment, were assessed. The results were compared with a panel of various oxidative stress and inflammatory biomarkers. The lambs were divided into three groups: animals highly infected (EPG higher than 5000) and packed cell volume (PCV) lower than 24% (G1); animals highly infected (EPG higher than 5000) and normal PCV (>24%) (G2); and animals presenting EPG lower than 5000 and normal PCV (>24%) (G3). The highly infected lambs (G1 and G2) showed lower total thiol (TT) and native thiol (SH) (p ≤ 0.01) than those from G3. After treatment, TT and SH increased significantly in all groups (p ≤ 0.01), and the disulphide (SS)/TT and SS/SH ratios decreased significantly (p < 0.01) in G1 and G2. These results show that the thiol-disulphide balance was impaired in lambs infected by gastrointestinal nematodes and that it could be potentially used as a biomarker to monitor this disease. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-29T08:34:50Z 2022-04-29T08:34:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ani11102856 Animals, v. 11, n. 10, 2021. 2076-2615 http://hdl.handle.net/11449/229616 10.3390/ani11102856 2-s2.0-85116015795 |
url |
http://dx.doi.org/10.3390/ani11102856 http://hdl.handle.net/11449/229616 |
identifier_str_mv |
Animals, v. 11, n. 10, 2021. 2076-2615 10.3390/ani11102856 2-s2.0-85116015795 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1822183855818276864 |
dc.identifier.doi.none.fl_str_mv |
10.3390/ani11102856 |