Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/JPER.20-0613 http://hdl.handle.net/11449/208252 |
Resumo: | Background: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical-size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5-mm-diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low-viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high-viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups (P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles (P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG (P ≤0.05). Increased number of TRAP-positive cells was observed in DBG/LV and DBG/HV (P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV (P ≤0.05). Conclusion: The combination of HA in a high-viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria. |
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Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defectsanimal experimentationbiomarkersbonebone regenerationbone transplantationBackground: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical-size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5-mm-diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low-viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high-viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups (P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles (P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG (P ≤0.05). Increased number of TRAP-positive cells was observed in DBG/LV and DBG/HV (P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV (P ≤0.05). Conclusion: The combination of HA in a high-viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria.Department of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP)Department of Basic Science School of Dentistry São Paulo State University (UNESP)Private practice. Director of the Pro-clinic Nucleus of Orofacial HarmonizationDepartment of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP)Department of Basic Science School of Dentistry São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Private practice. Director of the Pro-clinic Nucleus of Orofacial HarmonizationMatheus, Henrique R [UNESP]Ervolino, Edilson [UNESP]Gusman, David Jonathan Rodrigues [UNESP]Alves, Breno Edson Sendão [UNESP]Fiorin, Luiz Guilherme [UNESP]Pereira, Priscilla Aparecidade Almeida, Juliano Milanezi [UNESP]2021-06-25T11:09:07Z2021-06-25T11:09:07Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/JPER.20-0613Journal of Periodontology.0022-3492http://hdl.handle.net/11449/20825210.1002/JPER.20-06132-s2.0-85097763783Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Periodontologyinfo:eu-repo/semantics/openAccess2021-10-23T18:56:55Zoai:repositorio.unesp.br:11449/208252Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T18:56:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
title |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
spellingShingle |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects Matheus, Henrique R [UNESP] animal experimentation biomarkers bone bone regeneration bone transplantation |
title_short |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
title_full |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
title_fullStr |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
title_full_unstemmed |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
title_sort |
Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects |
author |
Matheus, Henrique R [UNESP] |
author_facet |
Matheus, Henrique R [UNESP] Ervolino, Edilson [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Alves, Breno Edson Sendão [UNESP] Fiorin, Luiz Guilherme [UNESP] Pereira, Priscilla Aparecida de Almeida, Juliano Milanezi [UNESP] |
author_role |
author |
author2 |
Ervolino, Edilson [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Alves, Breno Edson Sendão [UNESP] Fiorin, Luiz Guilherme [UNESP] Pereira, Priscilla Aparecida de Almeida, Juliano Milanezi [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Private practice. Director of the Pro-clinic Nucleus of Orofacial Harmonization |
dc.contributor.author.fl_str_mv |
Matheus, Henrique R [UNESP] Ervolino, Edilson [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Alves, Breno Edson Sendão [UNESP] Fiorin, Luiz Guilherme [UNESP] Pereira, Priscilla Aparecida de Almeida, Juliano Milanezi [UNESP] |
dc.subject.por.fl_str_mv |
animal experimentation biomarkers bone bone regeneration bone transplantation |
topic |
animal experimentation biomarkers bone bone regeneration bone transplantation |
description |
Background: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical-size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5-mm-diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low-viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high-viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups (P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles (P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG (P ≤0.05). Increased number of TRAP-positive cells was observed in DBG/LV and DBG/HV (P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV (P ≤0.05). Conclusion: The combination of HA in a high-viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T11:09:07Z 2021-06-25T11:09:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/JPER.20-0613 Journal of Periodontology. 0022-3492 http://hdl.handle.net/11449/208252 10.1002/JPER.20-0613 2-s2.0-85097763783 |
url |
http://dx.doi.org/10.1002/JPER.20-0613 http://hdl.handle.net/11449/208252 |
identifier_str_mv |
Journal of Periodontology. 0022-3492 10.1002/JPER.20-0613 2-s2.0-85097763783 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Periodontology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803046394263502848 |