A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations

Detalhes bibliográficos
Autor(a) principal: Vannucci Nunes, M. [UNESP]
Data de Publicação: 1995
Outros Autores: Barbosa De Melo, M. [UNESP], Ferreira Costa, F. [UNESP], Varella-Garcia, M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/223991
Resumo: In order to rescue molecular information from chromosome preparations, we describe a rapid procedure to obtain DNA from cytogenetic preparations in microscope slides, stored for one to five years at room temperature. This technique was modified from previously described procedures and the DNA obtained was shown to be suitable for PCR amplification.
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spelling A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparationsIn order to rescue molecular information from chromosome preparations, we describe a rapid procedure to obtain DNA from cytogenetic preparations in microscope slides, stored for one to five years at room temperature. This technique was modified from previously described procedures and the DNA obtained was shown to be suitable for PCR amplification.IBILCE Departamento de Biologia UNESP, Caixa Postal 136, 15054-000 Sao Jose do Rio Preto, SPIBILCE Departamento de Biologia UNESP, Caixa Postal 136, 15054-000 Sao Jose do Rio Preto, SPUniversidade Estadual Paulista (UNESP)Vannucci Nunes, M. [UNESP]Barbosa De Melo, M. [UNESP]Ferreira Costa, F. [UNESP]Varella-Garcia, M. [UNESP]2022-04-28T19:54:03Z2022-04-28T19:54:03Z1995-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article489-490Brazilian Journal of Genetics, v. 18, n. 3, p. 489-490, 1995.0100-8455http://hdl.handle.net/11449/2239912-s2.0-0028806762Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Geneticsinfo:eu-repo/semantics/openAccess2022-04-28T19:54:03Zoai:repositorio.unesp.br:11449/223991Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:07:47.345435Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
title A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
spellingShingle A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
Vannucci Nunes, M. [UNESP]
title_short A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
title_full A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
title_fullStr A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
title_full_unstemmed A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
title_sort A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations
author Vannucci Nunes, M. [UNESP]
author_facet Vannucci Nunes, M. [UNESP]
Barbosa De Melo, M. [UNESP]
Ferreira Costa, F. [UNESP]
Varella-Garcia, M. [UNESP]
author_role author
author2 Barbosa De Melo, M. [UNESP]
Ferreira Costa, F. [UNESP]
Varella-Garcia, M. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Vannucci Nunes, M. [UNESP]
Barbosa De Melo, M. [UNESP]
Ferreira Costa, F. [UNESP]
Varella-Garcia, M. [UNESP]
description In order to rescue molecular information from chromosome preparations, we describe a rapid procedure to obtain DNA from cytogenetic preparations in microscope slides, stored for one to five years at room temperature. This technique was modified from previously described procedures and the DNA obtained was shown to be suitable for PCR amplification.
publishDate 1995
dc.date.none.fl_str_mv 1995-01-01
2022-04-28T19:54:03Z
2022-04-28T19:54:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Journal of Genetics, v. 18, n. 3, p. 489-490, 1995.
0100-8455
http://hdl.handle.net/11449/223991
2-s2.0-0028806762
identifier_str_mv Brazilian Journal of Genetics, v. 18, n. 3, p. 489-490, 1995.
0100-8455
2-s2.0-0028806762
url http://hdl.handle.net/11449/223991
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Genetics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 489-490
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129587958251520

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