A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus

Detalhes bibliográficos
Autor(a) principal: Basso, Caroline R. [UNESP]
Data de Publicação: 2019
Outros Autores: Crulhas, Bruno P. [UNESP], Magro, Massimiliano, Vianello, Fabio, Pedrosa, Valber A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.talanta.2019.01.058
http://hdl.handle.net/11449/187289
Resumo: A new immunosensor using hybrid nanomaterials for the detection of dengue virus was demonstrated in this work. This immunosensor composed of nanoparticles of γ-Fe2O3(SAMN) modified with MPA- SAMN@MPA was characterized by FTIR spectroscopy, transmission electron microscopy,quartz crystal microbalance, UV–vis and LSPR technique. The binding of SAMN@MPA with AuNPs conjugated with aptamers(SAMN@MPA@AuNPs@aptamer) provides specific chemical bonds to four dengue serotypes. Colorimetric changes in the modification steps provided rapid visual detection of the virus without the use of equipment. Variations of aptamers concentrations 1.0-10.0 μM where the 3.0 μM aptamer concentration is sufficient to completely cover the surface of the modified AuNPs with an R2 value of> 0.99. This new proposed methodology presenting some advantages in relation to traditional detection methods such as time optimization and cost,can be used as a diagnostic method.
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spelling A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virusDengue virusHybrid nanomaterialLocalized surface plasmon resonanceA new immunosensor using hybrid nanomaterials for the detection of dengue virus was demonstrated in this work. This immunosensor composed of nanoparticles of γ-Fe2O3(SAMN) modified with MPA- SAMN@MPA was characterized by FTIR spectroscopy, transmission electron microscopy,quartz crystal microbalance, UV–vis and LSPR technique. The binding of SAMN@MPA with AuNPs conjugated with aptamers(SAMN@MPA@AuNPs@aptamer) provides specific chemical bonds to four dengue serotypes. Colorimetric changes in the modification steps provided rapid visual detection of the virus without the use of equipment. Variations of aptamers concentrations 1.0-10.0 μM where the 3.0 μM aptamer concentration is sufficient to completely cover the surface of the modified AuNPs with an R2 value of> 0.99. This new proposed methodology presenting some advantages in relation to traditional detection methods such as time optimization and cost,can be used as a diagnostic method.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Bioscience Department of Chemistry and Biochemistry UNESPDepartment of Comparative Biomedicine and Food Science University of PaduaInstitute of Bioscience Department of Chemistry and Biochemistry UNESPUniversidade Estadual Paulista (Unesp)University of PaduaBasso, Caroline R. [UNESP]Crulhas, Bruno P. [UNESP]Magro, MassimilianoVianello, FabioPedrosa, Valber A. [UNESP]2019-10-06T15:31:33Z2019-10-06T15:31:33Z2019-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article482-490http://dx.doi.org/10.1016/j.talanta.2019.01.058Talanta, v. 197, p. 482-490.0039-9140http://hdl.handle.net/11449/18728910.1016/j.talanta.2019.01.0582-s2.0-85060475512Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTalantainfo:eu-repo/semantics/openAccess2021-10-22T21:15:50Zoai:repositorio.unesp.br:11449/187289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:32.346588Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
title A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
spellingShingle A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
Basso, Caroline R. [UNESP]
Dengue virus
Hybrid nanomaterial
Localized surface plasmon resonance
title_short A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
title_full A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
title_fullStr A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
title_full_unstemmed A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
title_sort A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus
author Basso, Caroline R. [UNESP]
author_facet Basso, Caroline R. [UNESP]
Crulhas, Bruno P. [UNESP]
Magro, Massimiliano
Vianello, Fabio
Pedrosa, Valber A. [UNESP]
author_role author
author2 Crulhas, Bruno P. [UNESP]
Magro, Massimiliano
Vianello, Fabio
Pedrosa, Valber A. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Padua
dc.contributor.author.fl_str_mv Basso, Caroline R. [UNESP]
Crulhas, Bruno P. [UNESP]
Magro, Massimiliano
Vianello, Fabio
Pedrosa, Valber A. [UNESP]
dc.subject.por.fl_str_mv Dengue virus
Hybrid nanomaterial
Localized surface plasmon resonance
topic Dengue virus
Hybrid nanomaterial
Localized surface plasmon resonance
description A new immunosensor using hybrid nanomaterials for the detection of dengue virus was demonstrated in this work. This immunosensor composed of nanoparticles of γ-Fe2O3(SAMN) modified with MPA- SAMN@MPA was characterized by FTIR spectroscopy, transmission electron microscopy,quartz crystal microbalance, UV–vis and LSPR technique. The binding of SAMN@MPA with AuNPs conjugated with aptamers(SAMN@MPA@AuNPs@aptamer) provides specific chemical bonds to four dengue serotypes. Colorimetric changes in the modification steps provided rapid visual detection of the virus without the use of equipment. Variations of aptamers concentrations 1.0-10.0 μM where the 3.0 μM aptamer concentration is sufficient to completely cover the surface of the modified AuNPs with an R2 value of> 0.99. This new proposed methodology presenting some advantages in relation to traditional detection methods such as time optimization and cost,can be used as a diagnostic method.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:31:33Z
2019-10-06T15:31:33Z
2019-05-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.talanta.2019.01.058
Talanta, v. 197, p. 482-490.
0039-9140
http://hdl.handle.net/11449/187289
10.1016/j.talanta.2019.01.058
2-s2.0-85060475512
url http://dx.doi.org/10.1016/j.talanta.2019.01.058
http://hdl.handle.net/11449/187289
identifier_str_mv Talanta, v. 197, p. 482-490.
0039-9140
10.1016/j.talanta.2019.01.058
2-s2.0-85060475512
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Talanta
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 482-490
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128524402294784

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