Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems
Main Author: | |
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Publication Date: | 2020 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.ecoenv.2020.110778 http://hdl.handle.net/11449/201800 |
Summary: | Field peas (Pisum sativum L.) are widely cultivated throughout the world as a cool season grain and forage crop. Boron (B) toxicity is caused by high B concentration in the soil or irrigation water, and is particularly problematic in medium or heavier textured soil types with moderate alkalinity and low annual rainfall. Previous studies have indicated that B-toxicity increases oxidative stress in plants, and B-tolerance has been considered an important target in field pea plant breeding programmes. Inducers of tolerance may be a promising alternative for plant breeding. Little research has been conducted on the combined use of silicon (Si) and salicylic acid (SA) to remediate B-toxicity in field peas. The present study revealed the physiological and biochemical plant responses of applying Si + SA under B-toxicity (15 mg B L−1) on two Brazilian field pea cultivars (Iapar 83 and BRS Forrageira). A semi-hydroponic experiment was conducted using a completely randomized factorial design (2 × 5): with two field pea cultivars and five treatments which were formed by individual and combined applications of Si and SA under B-toxicity plus a control (control, B, B + Si, B + SA, and B + Si + SA). Si (2 mmol L−1) was applied to plants in two forms (root and leaf), while for SA (36 μmol L−1) only foliar applications were applied. Our results demonstrated that the combined use of exogenous Si + SA in field peas increased tolerance to B-toxicity through an intensified antioxidant plant defence system, resulting in a better regulation of reactive oxygen species (ROS) production and degradation. It significantly increased total chlorophyll and carotenoids contents, the activities of major antioxidant enzymes, and reduced MDA and H2O2 contents, resulting in increased fresh shoot and total plant dry biomass. The application of Si + SA alleviated the inhibitory effects of boron toxicity in field peas, resulting in greater plant growth by preventing oxidative membrane damage through an increased tolerance to B-excess within the plant tissue. Therefore, the use of Si + SA is an important and sustainable strategy to alleviate B-toxicity in field pea cultivation. |
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Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systemsAntioxidant responseBoron toxicityOxidative damagePisum sativum (L.)Salicylic acidSiliconField peas (Pisum sativum L.) are widely cultivated throughout the world as a cool season grain and forage crop. Boron (B) toxicity is caused by high B concentration in the soil or irrigation water, and is particularly problematic in medium or heavier textured soil types with moderate alkalinity and low annual rainfall. Previous studies have indicated that B-toxicity increases oxidative stress in plants, and B-tolerance has been considered an important target in field pea plant breeding programmes. Inducers of tolerance may be a promising alternative for plant breeding. Little research has been conducted on the combined use of silicon (Si) and salicylic acid (SA) to remediate B-toxicity in field peas. The present study revealed the physiological and biochemical plant responses of applying Si + SA under B-toxicity (15 mg B L−1) on two Brazilian field pea cultivars (Iapar 83 and BRS Forrageira). A semi-hydroponic experiment was conducted using a completely randomized factorial design (2 × 5): with two field pea cultivars and five treatments which were formed by individual and combined applications of Si and SA under B-toxicity plus a control (control, B, B + Si, B + SA, and B + Si + SA). Si (2 mmol L−1) was applied to plants in two forms (root and leaf), while for SA (36 μmol L−1) only foliar applications were applied. Our results demonstrated that the combined use of exogenous Si + SA in field peas increased tolerance to B-toxicity through an intensified antioxidant plant defence system, resulting in a better regulation of reactive oxygen species (ROS) production and degradation. It significantly increased total chlorophyll and carotenoids contents, the activities of major antioxidant enzymes, and reduced MDA and H2O2 contents, resulting in increased fresh shoot and total plant dry biomass. The application of Si + SA alleviated the inhibitory effects of boron toxicity in field peas, resulting in greater plant growth by preventing oxidative membrane damage through an increased tolerance to B-excess within the plant tissue. Therefore, the use of Si + SA is an important and sustainable strategy to alleviate B-toxicity in field pea cultivation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP). Faculty of Agrarian and Veterinary Sciences. Department of Biology Applied to AgricultureSão Paulo State University (UNESP). Faculty of Agrarian and Veterinary Sciences. Department of Agricultural Production SciencesCurtin University. School of Molecular and Life Sciences, GPO Box U1987Semi-Arid National Institute (INSA). Crop Production CenterSão Paulo State University (UNESP). Faculty of Agrarian and Veterinary Sciences. Department of Biology Applied to AgricultureSão Paulo State University (UNESP). Faculty of Agrarian and Veterinary Sciences. Department of Agricultural Production SciencesFAPESP: 2017/04787-6CNPq: 314380/2018–3Universidade Estadual Paulista (Unesp)Curtin University. School of Molecular and Life SciencesSemi-Arid National Institute (INSA). Crop Production CenterOliveira, Kevein Ruas [UNESP]Souza Junior, Jonas Pereira [UNESP]Bennett, Sarita JaneChecchio, Mirela Vantini [UNESP]Alves, Rita de CássiaFelisberto, Guilherme [UNESP]Prado, Renato de Mello [UNESP]Gratão, Priscila Lupino [UNESP]2020-12-12T02:42:09Z2020-12-12T02:42:09Z2020-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecoenv.2020.110778Ecotoxicology and Environmental Safety, v. 201.1090-24140147-6513http://hdl.handle.net/11449/20180010.1016/j.ecoenv.2020.1107782-s2.0-85085308981Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safetyinfo:eu-repo/semantics/openAccess2021-10-22T22:17:22Zoai:repositorio.unesp.br:11449/201800Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T22:17:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
title |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
spellingShingle |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems Oliveira, Kevein Ruas [UNESP] Antioxidant response Boron toxicity Oxidative damage Pisum sativum (L.) Salicylic acid Silicon |
title_short |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
title_full |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
title_fullStr |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
title_full_unstemmed |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
title_sort |
Exogenous silicon and salicylic acid applications improve tolerance to boron toxicity in field pea cultivars by intensifying antioxidant defence systems |
author |
Oliveira, Kevein Ruas [UNESP] |
author_facet |
Oliveira, Kevein Ruas [UNESP] Souza Junior, Jonas Pereira [UNESP] Bennett, Sarita Jane Checchio, Mirela Vantini [UNESP] Alves, Rita de Cássia Felisberto, Guilherme [UNESP] Prado, Renato de Mello [UNESP] Gratão, Priscila Lupino [UNESP] |
author_role |
author |
author2 |
Souza Junior, Jonas Pereira [UNESP] Bennett, Sarita Jane Checchio, Mirela Vantini [UNESP] Alves, Rita de Cássia Felisberto, Guilherme [UNESP] Prado, Renato de Mello [UNESP] Gratão, Priscila Lupino [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Curtin University. School of Molecular and Life Sciences Semi-Arid National Institute (INSA). Crop Production Center |
dc.contributor.author.fl_str_mv |
Oliveira, Kevein Ruas [UNESP] Souza Junior, Jonas Pereira [UNESP] Bennett, Sarita Jane Checchio, Mirela Vantini [UNESP] Alves, Rita de Cássia Felisberto, Guilherme [UNESP] Prado, Renato de Mello [UNESP] Gratão, Priscila Lupino [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant response Boron toxicity Oxidative damage Pisum sativum (L.) Salicylic acid Silicon |
topic |
Antioxidant response Boron toxicity Oxidative damage Pisum sativum (L.) Salicylic acid Silicon |
description |
Field peas (Pisum sativum L.) are widely cultivated throughout the world as a cool season grain and forage crop. Boron (B) toxicity is caused by high B concentration in the soil or irrigation water, and is particularly problematic in medium or heavier textured soil types with moderate alkalinity and low annual rainfall. Previous studies have indicated that B-toxicity increases oxidative stress in plants, and B-tolerance has been considered an important target in field pea plant breeding programmes. Inducers of tolerance may be a promising alternative for plant breeding. Little research has been conducted on the combined use of silicon (Si) and salicylic acid (SA) to remediate B-toxicity in field peas. The present study revealed the physiological and biochemical plant responses of applying Si + SA under B-toxicity (15 mg B L−1) on two Brazilian field pea cultivars (Iapar 83 and BRS Forrageira). A semi-hydroponic experiment was conducted using a completely randomized factorial design (2 × 5): with two field pea cultivars and five treatments which were formed by individual and combined applications of Si and SA under B-toxicity plus a control (control, B, B + Si, B + SA, and B + Si + SA). Si (2 mmol L−1) was applied to plants in two forms (root and leaf), while for SA (36 μmol L−1) only foliar applications were applied. Our results demonstrated that the combined use of exogenous Si + SA in field peas increased tolerance to B-toxicity through an intensified antioxidant plant defence system, resulting in a better regulation of reactive oxygen species (ROS) production and degradation. It significantly increased total chlorophyll and carotenoids contents, the activities of major antioxidant enzymes, and reduced MDA and H2O2 contents, resulting in increased fresh shoot and total plant dry biomass. The application of Si + SA alleviated the inhibitory effects of boron toxicity in field peas, resulting in greater plant growth by preventing oxidative membrane damage through an increased tolerance to B-excess within the plant tissue. Therefore, the use of Si + SA is an important and sustainable strategy to alleviate B-toxicity in field pea cultivation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:42:09Z 2020-12-12T02:42:09Z 2020-09-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2020.110778 Ecotoxicology and Environmental Safety, v. 201. 1090-2414 0147-6513 http://hdl.handle.net/11449/201800 10.1016/j.ecoenv.2020.110778 2-s2.0-85085308981 |
url |
http://dx.doi.org/10.1016/j.ecoenv.2020.110778 http://hdl.handle.net/11449/201800 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 201. 1090-2414 0147-6513 10.1016/j.ecoenv.2020.110778 2-s2.0-85085308981 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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