Survey of viruses belonging to different genera and species in noble garlic in Brazil

Detalhes bibliográficos
Autor(a) principal: Mituti, Tatiana [UNESP]
Data de Publicação: 2015
Outros Autores: Moura, Monika Fecury [UNESP], Marubayashi, Julio Massaharu [UNESP], Oliveira, Milena Leite [UNESP], Imaizumi, Vitor Massami [UNESP], Sakate, Renate Krause [UNESP], Pavan, Marcelo Agenor [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129280
Resumo: Garlic (Allium sativum L.) is a host to several viruses, most commonly those belonging to the Allexivirus, Carlavirus, or Potyvirus genera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses were Onion yellow dwarf virus (OYDV, 56 %) and Leek yellow stripe virus (LYSV, 55 %). 187 samples (49 %) were positive for allexivirus, with 33 (9 %) showing single infections and 154 (41 %) showing mixed infections with Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and species belonging to the Carlavirus and Potyvirus genera. The predominant species in which allexiviruses were found were GarV-A and GarV-D. Only 15 samples (4 %) were infected solely by a carlavirus, and 63 (17 %) showed mixed infections with viruses from different genera. The dominant species of carlavirus was Garlic commom latent virus (GarCLV). Carlaviruses and allexiviruses are frequently associated with mixed infections with potyviruses, whereas mixed infections with carlaviruses and allexiviruses are rare. About 70 % of the plants collected were positive for at least one species of virus.
id UNSP_a81f167a9265c2eea0d8551295fae628
oai_identifier_str oai:repositorio.unesp.br:11449/129280
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Survey of viruses belonging to different genera and species in noble garlic in BrazilAllexivirusAllium sativumCarlavirusPotyvirusoccurrenceGarlic (Allium sativum L.) is a host to several viruses, most commonly those belonging to the Allexivirus, Carlavirus, or Potyvirus genera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses were Onion yellow dwarf virus (OYDV, 56 %) and Leek yellow stripe virus (LYSV, 55 %). 187 samples (49 %) were positive for allexivirus, with 33 (9 %) showing single infections and 154 (41 %) showing mixed infections with Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and species belonging to the Carlavirus and Potyvirus genera. The predominant species in which allexiviruses were found were GarV-A and GarV-D. Only 15 samples (4 %) were infected solely by a carlavirus, and 63 (17 %) showed mixed infections with viruses from different genera. The dominant species of carlavirus was Garlic commom latent virus (GarCLV). Carlaviruses and allexiviruses are frequently associated with mixed infections with potyviruses, whereas mixed infections with carlaviruses and allexiviruses are rare. About 70 % of the plants collected were positive for at least one species of virus.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ FCA, Dept Plant Protect, BR-18610307 Botucatu, SP, BrazilSao Paulo State Univ FCA, Dept Plant Protect, BR-18610307 Botucatu, SP, BrazilFAPESP: 2010/16148-9CNPq: 472032/2010-0CAPES: 5279/11-9Univ Sao PaoloUniversidade Estadual Paulista (Unesp)Mituti, Tatiana [UNESP]Moura, Monika Fecury [UNESP]Marubayashi, Julio Massaharu [UNESP]Oliveira, Milena Leite [UNESP]Imaizumi, Vitor Massami [UNESP]Sakate, Renate Krause [UNESP]Pavan, Marcelo Agenor [UNESP]2015-10-21T20:45:01Z2015-10-21T20:45:01Z2015-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article278-281application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=enScientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 72, n. 3, p. 278-281, 2015.0103-9016http://hdl.handle.net/11449/12928010.1590/0103-9016-2014-0168S0103-90162015000300278WOS:000357631600012S0103-90162015000300278.pdf965982285569768594756645633629490000-0001-7526-640XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientia Agricola0,578info:eu-repo/semantics/openAccess2024-04-30T18:07:56Zoai:repositorio.unesp.br:11449/129280Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:03:39.692040Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Survey of viruses belonging to different genera and species in noble garlic in Brazil
title Survey of viruses belonging to different genera and species in noble garlic in Brazil
spellingShingle Survey of viruses belonging to different genera and species in noble garlic in Brazil
Mituti, Tatiana [UNESP]
Allexivirus
Allium sativum
Carlavirus
Potyvirus
occurrence
title_short Survey of viruses belonging to different genera and species in noble garlic in Brazil
title_full Survey of viruses belonging to different genera and species in noble garlic in Brazil
title_fullStr Survey of viruses belonging to different genera and species in noble garlic in Brazil
title_full_unstemmed Survey of viruses belonging to different genera and species in noble garlic in Brazil
title_sort Survey of viruses belonging to different genera and species in noble garlic in Brazil
author Mituti, Tatiana [UNESP]
author_facet Mituti, Tatiana [UNESP]
Moura, Monika Fecury [UNESP]
Marubayashi, Julio Massaharu [UNESP]
Oliveira, Milena Leite [UNESP]
Imaizumi, Vitor Massami [UNESP]
Sakate, Renate Krause [UNESP]
Pavan, Marcelo Agenor [UNESP]
author_role author
author2 Moura, Monika Fecury [UNESP]
Marubayashi, Julio Massaharu [UNESP]
Oliveira, Milena Leite [UNESP]
Imaizumi, Vitor Massami [UNESP]
Sakate, Renate Krause [UNESP]
Pavan, Marcelo Agenor [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Mituti, Tatiana [UNESP]
Moura, Monika Fecury [UNESP]
Marubayashi, Julio Massaharu [UNESP]
Oliveira, Milena Leite [UNESP]
Imaizumi, Vitor Massami [UNESP]
Sakate, Renate Krause [UNESP]
Pavan, Marcelo Agenor [UNESP]
dc.subject.por.fl_str_mv Allexivirus
Allium sativum
Carlavirus
Potyvirus
occurrence
topic Allexivirus
Allium sativum
Carlavirus
Potyvirus
occurrence
description Garlic (Allium sativum L.) is a host to several viruses, most commonly those belonging to the Allexivirus, Carlavirus, or Potyvirus genera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses were Onion yellow dwarf virus (OYDV, 56 %) and Leek yellow stripe virus (LYSV, 55 %). 187 samples (49 %) were positive for allexivirus, with 33 (9 %) showing single infections and 154 (41 %) showing mixed infections with Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and species belonging to the Carlavirus and Potyvirus genera. The predominant species in which allexiviruses were found were GarV-A and GarV-D. Only 15 samples (4 %) were infected solely by a carlavirus, and 63 (17 %) showed mixed infections with viruses from different genera. The dominant species of carlavirus was Garlic commom latent virus (GarCLV). Carlaviruses and allexiviruses are frequently associated with mixed infections with potyviruses, whereas mixed infections with carlaviruses and allexiviruses are rare. About 70 % of the plants collected were positive for at least one species of virus.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T20:45:01Z
2015-10-21T20:45:01Z
2015-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=en
Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 72, n. 3, p. 278-281, 2015.
0103-9016
http://hdl.handle.net/11449/129280
10.1590/0103-9016-2014-0168
S0103-90162015000300278
WOS:000357631600012
S0103-90162015000300278.pdf
9659822855697685
9475664563362949
0000-0001-7526-640X
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129280
identifier_str_mv Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 72, n. 3, p. 278-281, 2015.
0103-9016
10.1590/0103-9016-2014-0168
S0103-90162015000300278
WOS:000357631600012
S0103-90162015000300278.pdf
9659822855697685
9475664563362949
0000-0001-7526-640X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientia Agricola
0,578
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 278-281
application/pdf
dc.publisher.none.fl_str_mv Univ Sao Paolo
publisher.none.fl_str_mv Univ Sao Paolo
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129578070179840