Survey of viruses belonging to different genera and species in noble garlic in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/129280 |
Resumo: | Garlic (Allium sativum L.) is a host to several viruses, most commonly those belonging to the Allexivirus, Carlavirus, or Potyvirus genera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses were Onion yellow dwarf virus (OYDV, 56 %) and Leek yellow stripe virus (LYSV, 55 %). 187 samples (49 %) were positive for allexivirus, with 33 (9 %) showing single infections and 154 (41 %) showing mixed infections with Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and species belonging to the Carlavirus and Potyvirus genera. The predominant species in which allexiviruses were found were GarV-A and GarV-D. Only 15 samples (4 %) were infected solely by a carlavirus, and 63 (17 %) showed mixed infections with viruses from different genera. The dominant species of carlavirus was Garlic commom latent virus (GarCLV). Carlaviruses and allexiviruses are frequently associated with mixed infections with potyviruses, whereas mixed infections with carlaviruses and allexiviruses are rare. About 70 % of the plants collected were positive for at least one species of virus. |
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Survey of viruses belonging to different genera and species in noble garlic in BrazilAllexivirusAllium sativumCarlavirusPotyvirusoccurrenceGarlic (Allium sativum L.) is a host to several viruses, most commonly those belonging to the Allexivirus, Carlavirus, or Potyvirus genera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses were Onion yellow dwarf virus (OYDV, 56 %) and Leek yellow stripe virus (LYSV, 55 %). 187 samples (49 %) were positive for allexivirus, with 33 (9 %) showing single infections and 154 (41 %) showing mixed infections with Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and species belonging to the Carlavirus and Potyvirus genera. The predominant species in which allexiviruses were found were GarV-A and GarV-D. Only 15 samples (4 %) were infected solely by a carlavirus, and 63 (17 %) showed mixed infections with viruses from different genera. The dominant species of carlavirus was Garlic commom latent virus (GarCLV). Carlaviruses and allexiviruses are frequently associated with mixed infections with potyviruses, whereas mixed infections with carlaviruses and allexiviruses are rare. About 70 % of the plants collected were positive for at least one species of virus.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ FCA, Dept Plant Protect, BR-18610307 Botucatu, SP, BrazilSao Paulo State Univ FCA, Dept Plant Protect, BR-18610307 Botucatu, SP, BrazilFAPESP: 2010/16148-9CNPq: 472032/2010-0CAPES: 5279/11-9Univ Sao PaoloUniversidade Estadual Paulista (Unesp)Mituti, Tatiana [UNESP]Moura, Monika Fecury [UNESP]Marubayashi, Julio Massaharu [UNESP]Oliveira, Milena Leite [UNESP]Imaizumi, Vitor Massami [UNESP]Sakate, Renate Krause [UNESP]Pavan, Marcelo Agenor [UNESP]2015-10-21T20:45:01Z2015-10-21T20:45:01Z2015-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article278-281application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=enScientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 72, n. 3, p. 278-281, 2015.0103-9016http://hdl.handle.net/11449/12928010.1590/0103-9016-2014-0168S0103-90162015000300278WOS:000357631600012S0103-90162015000300278.pdf965982285569768594756645633629490000-0001-7526-640XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientia Agricola0,578info:eu-repo/semantics/openAccess2024-04-30T18:07:56Zoai:repositorio.unesp.br:11449/129280Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:03:39.692040Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
title |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
spellingShingle |
Survey of viruses belonging to different genera and species in noble garlic in Brazil Mituti, Tatiana [UNESP] Allexivirus Allium sativum Carlavirus Potyvirus occurrence |
title_short |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
title_full |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
title_fullStr |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
title_full_unstemmed |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
title_sort |
Survey of viruses belonging to different genera and species in noble garlic in Brazil |
author |
Mituti, Tatiana [UNESP] |
author_facet |
Mituti, Tatiana [UNESP] Moura, Monika Fecury [UNESP] Marubayashi, Julio Massaharu [UNESP] Oliveira, Milena Leite [UNESP] Imaizumi, Vitor Massami [UNESP] Sakate, Renate Krause [UNESP] Pavan, Marcelo Agenor [UNESP] |
author_role |
author |
author2 |
Moura, Monika Fecury [UNESP] Marubayashi, Julio Massaharu [UNESP] Oliveira, Milena Leite [UNESP] Imaizumi, Vitor Massami [UNESP] Sakate, Renate Krause [UNESP] Pavan, Marcelo Agenor [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mituti, Tatiana [UNESP] Moura, Monika Fecury [UNESP] Marubayashi, Julio Massaharu [UNESP] Oliveira, Milena Leite [UNESP] Imaizumi, Vitor Massami [UNESP] Sakate, Renate Krause [UNESP] Pavan, Marcelo Agenor [UNESP] |
dc.subject.por.fl_str_mv |
Allexivirus Allium sativum Carlavirus Potyvirus occurrence |
topic |
Allexivirus Allium sativum Carlavirus Potyvirus occurrence |
description |
Garlic (Allium sativum L.) is a host to several viruses, most commonly those belonging to the Allexivirus, Carlavirus, or Potyvirus genera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses were Onion yellow dwarf virus (OYDV, 56 %) and Leek yellow stripe virus (LYSV, 55 %). 187 samples (49 %) were positive for allexivirus, with 33 (9 %) showing single infections and 154 (41 %) showing mixed infections with Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C), Garlic virus D (GarV-D), and species belonging to the Carlavirus and Potyvirus genera. The predominant species in which allexiviruses were found were GarV-A and GarV-D. Only 15 samples (4 %) were infected solely by a carlavirus, and 63 (17 %) showed mixed infections with viruses from different genera. The dominant species of carlavirus was Garlic commom latent virus (GarCLV). Carlaviruses and allexiviruses are frequently associated with mixed infections with potyviruses, whereas mixed infections with carlaviruses and allexiviruses are rare. About 70 % of the plants collected were positive for at least one species of virus. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T20:45:01Z 2015-10-21T20:45:01Z 2015-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=en Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 72, n. 3, p. 278-281, 2015. 0103-9016 http://hdl.handle.net/11449/129280 10.1590/0103-9016-2014-0168 S0103-90162015000300278 WOS:000357631600012 S0103-90162015000300278.pdf 9659822855697685 9475664563362949 0000-0001-7526-640X |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/129280 |
identifier_str_mv |
Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 72, n. 3, p. 278-281, 2015. 0103-9016 10.1590/0103-9016-2014-0168 S0103-90162015000300278 WOS:000357631600012 S0103-90162015000300278.pdf 9659822855697685 9475664563362949 0000-0001-7526-640X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientia Agricola 0,578 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
278-281 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Sao Paolo |
publisher.none.fl_str_mv |
Univ Sao Paolo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129578070179840 |