Effects of eating disorders on oral fungal diversity

• Autor(a) principal: Back Brito, Graziella Nuernberg [UNESP]
• Data de Publicação: 2012
• Outros Autores: da Mota, Adolfo Jose, de Souza Bernardes, Luciano Angelo, Takamune, Silvia Satomi, Gomes Barbosa Prado, Eliane de Fatima, Cordas, Taki Athanassios, Balducci, Ivan [UNESP], da Nobrega, Francisco Gorgonio [UNESP], Koga-Ito, Cristiane Yumi [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.oooo.2011.10.007; http://hdl.handle.net/11449/22593

Background. The eating disorders anorexia and bulimia nervosa can cause several systemic and oral alterations related to poor nutrition and induced vomiting; however, the oral microflora of these patients is poorly studied.Objective. The aim of this study was to evaluate fungal microflora in the oral cavity of these patients by culture-dependent and culture-independent methods.Study Design. Oral rinse samples were cultured to assess the prevalence of Candida species, and the isolates were identified by API system. Microorganism counts were compared by the Mann-Whitney test (5%). Ribotyping, a type of molecular analysis, was performed by sequencing the D1/D2 regions of 28S rRNA.Results. Our results demonstrated that the eating disorder group showed higher oral Candida spp. prevalence with culture-dependent methods and higher species diversity with culture-independent methods.Conclusions. Eating disorders can lead to an increased oral Candida carriage. Culture-independent identification found greater fungal diversity than culture-dependent methods. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;113:512-517)