Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.indcrop.2019.111795 http://hdl.handle.net/11449/201202 |
Resumo: | Pycnoporus sanguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 °C. The highest endoglucanase, exoglucanase, β-glucosidase, xylanase, β-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g−1, respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g−1 for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L−1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 °C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained. |
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Repositório Institucional da UNESP |
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Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol productionCellulosic ethanolEnzymatic saccharificationLignocellulasesPretreatmentSolid-state fermentationPycnoporus sanguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 °C. The highest endoglucanase, exoglucanase, β-glucosidase, xylanase, β-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g−1, respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g−1 for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L−1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 °C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained.São Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) Institute of ChemistrySão Paulo State University (UNESP) Faculty of Pharmaceutical SciencesSão Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) Institute of ChemistrySão Paulo State University (UNESP) Faculty of Pharmaceutical SciencesUniversidade Estadual Paulista (Unesp)Pereira Scarpa, Josiani de Cassia [UNESP]Paganini Marques, Natália [UNESP]Alves Monteiro, Diego [UNESP]Martins, Gisele Marta [UNESP]Paula, Ariela Veloso de [UNESP]Boscolo, Maurício [UNESP]da Silva, Roberto [UNESP]Gomes, Eleni [UNESP]Alonso Bocchini, Daniela [UNESP]2020-12-12T02:26:39Z2020-12-12T02:26:39Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.indcrop.2019.111795Industrial Crops and Products, v. 141.0926-6690http://hdl.handle.net/11449/20120210.1016/j.indcrop.2019.1117952-s2.0-85072779398Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndustrial Crops and Productsinfo:eu-repo/semantics/openAccess2021-10-22T12:58:32Zoai:repositorio.unesp.br:11449/201202Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T12:58:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
title |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
spellingShingle |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production Pereira Scarpa, Josiani de Cassia [UNESP] Cellulosic ethanol Enzymatic saccharification Lignocellulases Pretreatment Solid-state fermentation |
title_short |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
title_full |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
title_fullStr |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
title_full_unstemmed |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
title_sort |
Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production |
author |
Pereira Scarpa, Josiani de Cassia [UNESP] |
author_facet |
Pereira Scarpa, Josiani de Cassia [UNESP] Paganini Marques, Natália [UNESP] Alves Monteiro, Diego [UNESP] Martins, Gisele Marta [UNESP] Paula, Ariela Veloso de [UNESP] Boscolo, Maurício [UNESP] da Silva, Roberto [UNESP] Gomes, Eleni [UNESP] Alonso Bocchini, Daniela [UNESP] |
author_role |
author |
author2 |
Paganini Marques, Natália [UNESP] Alves Monteiro, Diego [UNESP] Martins, Gisele Marta [UNESP] Paula, Ariela Veloso de [UNESP] Boscolo, Maurício [UNESP] da Silva, Roberto [UNESP] Gomes, Eleni [UNESP] Alonso Bocchini, Daniela [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pereira Scarpa, Josiani de Cassia [UNESP] Paganini Marques, Natália [UNESP] Alves Monteiro, Diego [UNESP] Martins, Gisele Marta [UNESP] Paula, Ariela Veloso de [UNESP] Boscolo, Maurício [UNESP] da Silva, Roberto [UNESP] Gomes, Eleni [UNESP] Alonso Bocchini, Daniela [UNESP] |
dc.subject.por.fl_str_mv |
Cellulosic ethanol Enzymatic saccharification Lignocellulases Pretreatment Solid-state fermentation |
topic |
Cellulosic ethanol Enzymatic saccharification Lignocellulases Pretreatment Solid-state fermentation |
description |
Pycnoporus sanguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 °C. The highest endoglucanase, exoglucanase, β-glucosidase, xylanase, β-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g−1, respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g−1 for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L−1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 °C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-12T02:26:39Z 2020-12-12T02:26:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.indcrop.2019.111795 Industrial Crops and Products, v. 141. 0926-6690 http://hdl.handle.net/11449/201202 10.1016/j.indcrop.2019.111795 2-s2.0-85072779398 |
url |
http://dx.doi.org/10.1016/j.indcrop.2019.111795 http://hdl.handle.net/11449/201202 |
identifier_str_mv |
Industrial Crops and Products, v. 141. 0926-6690 10.1016/j.indcrop.2019.111795 2-s2.0-85072779398 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Industrial Crops and Products |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789854697783296 |