Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/15287394.2021.1898505 http://hdl.handle.net/11449/210727 |
Resumo: | Copaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin. |
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Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo modelsCopaifera langsdorffiiafzelinquercitringenotoxicityantigenotoxicityCopaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Franca, Lab Mutagenese, Franca, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilFAPESP: 2011/1360-7Taylor & Francis IncUniv FrancaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Ozelin, Saulo DuarteSenedese, Juliana MarquesAlves, Jacqueline MoraisMunari, Carla CarolinaCosta, Juliana De Carvalho DaResende, Flavia Aparecida [UNESP]Campos, Debora Leite [UNESP]Lima, Ildercilio Mota De SouzaAndrade, Augusto FariaVaranda, Eliana Aparecida [UNESP]Bastos, Jairo KenuppTavares, Denise Crispim2021-06-26T04:30:37Z2021-06-26T04:30:37Z2021-03-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article569-581http://dx.doi.org/10.1080/15287394.2021.1898505Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 84, n. 14, p. 569-581, 2021.1528-7394http://hdl.handle.net/11449/21072710.1080/15287394.2021.1898505WOS:000630293500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Toxicology And Environmental Health-part A-current Issuesinfo:eu-repo/semantics/openAccess2024-06-24T13:07:52Zoai:repositorio.unesp.br:11449/210727Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:44:01.495081Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
title |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
spellingShingle |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models Ozelin, Saulo Duarte Copaifera langsdorffii afzelin quercitrin genotoxicity antigenotoxicity |
title_short |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
title_full |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
title_fullStr |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
title_full_unstemmed |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
title_sort |
Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models |
author |
Ozelin, Saulo Duarte |
author_facet |
Ozelin, Saulo Duarte Senedese, Juliana Marques Alves, Jacqueline Morais Munari, Carla Carolina Costa, Juliana De Carvalho Da Resende, Flavia Aparecida [UNESP] Campos, Debora Leite [UNESP] Lima, Ildercilio Mota De Souza Andrade, Augusto Faria Varanda, Eliana Aparecida [UNESP] Bastos, Jairo Kenupp Tavares, Denise Crispim |
author_role |
author |
author2 |
Senedese, Juliana Marques Alves, Jacqueline Morais Munari, Carla Carolina Costa, Juliana De Carvalho Da Resende, Flavia Aparecida [UNESP] Campos, Debora Leite [UNESP] Lima, Ildercilio Mota De Souza Andrade, Augusto Faria Varanda, Eliana Aparecida [UNESP] Bastos, Jairo Kenupp Tavares, Denise Crispim |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Franca Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ozelin, Saulo Duarte Senedese, Juliana Marques Alves, Jacqueline Morais Munari, Carla Carolina Costa, Juliana De Carvalho Da Resende, Flavia Aparecida [UNESP] Campos, Debora Leite [UNESP] Lima, Ildercilio Mota De Souza Andrade, Augusto Faria Varanda, Eliana Aparecida [UNESP] Bastos, Jairo Kenupp Tavares, Denise Crispim |
dc.subject.por.fl_str_mv |
Copaifera langsdorffii afzelin quercitrin genotoxicity antigenotoxicity |
topic |
Copaifera langsdorffii afzelin quercitrin genotoxicity antigenotoxicity |
description |
Copaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-26T04:30:37Z 2021-06-26T04:30:37Z 2021-03-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/15287394.2021.1898505 Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 84, n. 14, p. 569-581, 2021. 1528-7394 http://hdl.handle.net/11449/210727 10.1080/15287394.2021.1898505 WOS:000630293500001 |
url |
http://dx.doi.org/10.1080/15287394.2021.1898505 http://hdl.handle.net/11449/210727 |
identifier_str_mv |
Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 84, n. 14, p. 569-581, 2021. 1528-7394 10.1080/15287394.2021.1898505 WOS:000630293500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Toxicology And Environmental Health-part A-current Issues |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
569-581 |
dc.publisher.none.fl_str_mv |
Taylor & Francis Inc |
publisher.none.fl_str_mv |
Taylor & Francis Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129110316154880 |