Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models

Detalhes bibliográficos
Autor(a) principal: Ozelin, Saulo Duarte
Data de Publicação: 2021
Outros Autores: Senedese, Juliana Marques, Alves, Jacqueline Morais, Munari, Carla Carolina, Costa, Juliana De Carvalho Da, Resende, Flavia Aparecida [UNESP], Campos, Debora Leite [UNESP], Lima, Ildercilio Mota De Souza, Andrade, Augusto Faria, Varanda, Eliana Aparecida [UNESP], Bastos, Jairo Kenupp, Tavares, Denise Crispim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/15287394.2021.1898505
http://hdl.handle.net/11449/210727
Resumo: Copaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin.
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spelling Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo modelsCopaifera langsdorffiiafzelinquercitringenotoxicityantigenotoxicityCopaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Franca, Lab Mutagenese, Franca, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Araraquara, SP, BrazilFAPESP: 2011/1360-7Taylor & Francis IncUniv FrancaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Ozelin, Saulo DuarteSenedese, Juliana MarquesAlves, Jacqueline MoraisMunari, Carla CarolinaCosta, Juliana De Carvalho DaResende, Flavia Aparecida [UNESP]Campos, Debora Leite [UNESP]Lima, Ildercilio Mota De SouzaAndrade, Augusto FariaVaranda, Eliana Aparecida [UNESP]Bastos, Jairo KenuppTavares, Denise Crispim2021-06-26T04:30:37Z2021-06-26T04:30:37Z2021-03-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article569-581http://dx.doi.org/10.1080/15287394.2021.1898505Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 84, n. 14, p. 569-581, 2021.1528-7394http://hdl.handle.net/11449/21072710.1080/15287394.2021.1898505WOS:000630293500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Toxicology And Environmental Health-part A-current Issuesinfo:eu-repo/semantics/openAccess2024-06-24T13:07:52Zoai:repositorio.unesp.br:11449/210727Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:44:01.495081Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
title Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
spellingShingle Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
Ozelin, Saulo Duarte
Copaifera langsdorffii
afzelin
quercitrin
genotoxicity
antigenotoxicity
title_short Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
title_full Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
title_fullStr Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
title_full_unstemmed Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
title_sort Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models
author Ozelin, Saulo Duarte
author_facet Ozelin, Saulo Duarte
Senedese, Juliana Marques
Alves, Jacqueline Morais
Munari, Carla Carolina
Costa, Juliana De Carvalho Da
Resende, Flavia Aparecida [UNESP]
Campos, Debora Leite [UNESP]
Lima, Ildercilio Mota De Souza
Andrade, Augusto Faria
Varanda, Eliana Aparecida [UNESP]
Bastos, Jairo Kenupp
Tavares, Denise Crispim
author_role author
author2 Senedese, Juliana Marques
Alves, Jacqueline Morais
Munari, Carla Carolina
Costa, Juliana De Carvalho Da
Resende, Flavia Aparecida [UNESP]
Campos, Debora Leite [UNESP]
Lima, Ildercilio Mota De Souza
Andrade, Augusto Faria
Varanda, Eliana Aparecida [UNESP]
Bastos, Jairo Kenupp
Tavares, Denise Crispim
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Franca
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ozelin, Saulo Duarte
Senedese, Juliana Marques
Alves, Jacqueline Morais
Munari, Carla Carolina
Costa, Juliana De Carvalho Da
Resende, Flavia Aparecida [UNESP]
Campos, Debora Leite [UNESP]
Lima, Ildercilio Mota De Souza
Andrade, Augusto Faria
Varanda, Eliana Aparecida [UNESP]
Bastos, Jairo Kenupp
Tavares, Denise Crispim
dc.subject.por.fl_str_mv Copaifera langsdorffii
afzelin
quercitrin
genotoxicity
antigenotoxicity
topic Copaifera langsdorffii
afzelin
quercitrin
genotoxicity
antigenotoxicity
description Copaifera langsdorffii Desf. is a plant found in South America, especially in Brazil. Oleoresin and the leaves of this plant is used as a popular medicinal agent. However, few studies on the chemical composition of aerial parts and related biological activities are known. This study aimed to examine the cytotoxic, genotoxic, and antigenotoxic potential of C. langsdorffii aerial parts hydroalcoholic extract (CLE) and two of its major compounds afzelin and quercitrin. The cytotoxic and antigenotoxic potential of CLE was determined as follows: 1) against genotoxicity induced by doxorubicin (DXR) or methyl methanesulfonate (MMS) in V79 cells; 2) by direct and indirect-acting mutagens in Salmonella typhimurium strains; and 3) by MMS in male Swiss mice. The protective effects of afzelin and quercitrin against DXR or MMS were also evaluated in V79 and HepG2 cells. CLE was cytotoxic as evidenced by clonogenic efficiency assay. Further, CLE did not induce a significant change in frequencies of chromosomal aberrations and micronuclei; as well as number of revertants in the Ames test demonstrating absence of genotoxicity. In contrast, CLE was found to be antigenotoxic in mammalian cells. The results also showed that CLE exerted inhibitory effect against indirect-acting mutagens in the Ames test. Afzelin and quercitrin did not reduce genotoxicity induced by DXR or MMS in V79 cells. However, treatments using afzelin and quercitrin decreased MMS-induced genotoxicity in HepG2 cells. The antigenotoxic effect of CLE observed in this study may be partially attributed to the antioxidant activity of the combination of major components afzelin and quercitrin.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-26T04:30:37Z
2021-06-26T04:30:37Z
2021-03-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/15287394.2021.1898505
Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 84, n. 14, p. 569-581, 2021.
1528-7394
http://hdl.handle.net/11449/210727
10.1080/15287394.2021.1898505
WOS:000630293500001
url http://dx.doi.org/10.1080/15287394.2021.1898505
http://hdl.handle.net/11449/210727
identifier_str_mv Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 84, n. 14, p. 569-581, 2021.
1528-7394
10.1080/15287394.2021.1898505
WOS:000630293500001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Toxicology And Environmental Health-part A-current Issues
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 569-581
dc.publisher.none.fl_str_mv Taylor & Francis Inc
publisher.none.fl_str_mv Taylor & Francis Inc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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