Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Diego Luis
Data de Publicação: 2018
Outros Autores: Cilião, Heloísa Lizotti, Specian, Ana Flávia Leal, Serpeloni, Juliana Mara [UNESP], De Oliveira, Marcelo Tempesta, Varanda, Eliana Aparecida [UNESP], Vilegas, Wagner [UNESP], Saldanha, Luiz Leonardo [UNESP], Martínez-López, Wilner, Dokkedal, Anne Lígia [UNESP], Cólus, Ilce Mara Syllos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10616-017-0173-5
http://hdl.handle.net/11449/175624
Resumo: Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 µg/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 µg/mL was chosen to evaluate the protective effects against DNA damage induced by benzo[a]pyrene (B[a]P). At concentrations higher than 7.5 µg/mL (between 10 and 50 µg/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B[a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine.
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spelling Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitroAntimutagenicFlavonoidsFlow cytometryMicronucleusRT-qPCRBauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 µg/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 µg/mL was chosen to evaluate the protective effects against DNA damage induced by benzo[a]pyrene (B[a]P). At concentrations higher than 7.5 µg/mL (between 10 and 50 µg/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B[a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of General Biology Biological Sciences Center State University of Londrina - UEL, Rod Celso Garcia Cid PR 445, Km 380, University Campus, P.O. Box 6001Department of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University - UNESPCampus Litoral Paulista São Paulo State University - UNESPDepartment of Biological Sciences Faculty of Sciences of Bauru São Paulo State University – UNESPDepartment of Botany Biosciences Institute São Paulo State University - UNESPInstitute of Biological Investigation Clemente EstableDepartment of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University - UNESPCampus Litoral Paulista São Paulo State University - UNESPDepartment of Biological Sciences Faculty of Sciences of Bauru São Paulo State University – UNESPDepartment of Botany Biosciences Institute São Paulo State University - UNESPFAPESP: 2009/16147-5FAPESP: 2009/52237-9FAPESP: 2012/01996-0Universidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Institute of Biological Investigation Clemente EstableRibeiro, Diego LuisCilião, Heloísa LizottiSpecian, Ana Flávia LealSerpeloni, Juliana Mara [UNESP]De Oliveira, Marcelo TempestaVaranda, Eliana Aparecida [UNESP]Vilegas, Wagner [UNESP]Saldanha, Luiz Leonardo [UNESP]Martínez-López, WilnerDokkedal, Anne Lígia [UNESP]Cólus, Ilce Mara Syllos2018-12-11T17:16:46Z2018-12-11T17:16:46Z2018-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article713-728application/pdfhttp://dx.doi.org/10.1007/s10616-017-0173-5Cytotechnology, v. 70, n. 2, p. 713-728, 2018.1573-07780920-9069http://hdl.handle.net/11449/17562410.1007/s10616-017-0173-52-s2.0-850377173192-s2.0-85037717319.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytotechnology0,5190,519info:eu-repo/semantics/openAccess2024-06-24T13:07:39Zoai:repositorio.unesp.br:11449/175624Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:25:31.711735Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
title Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
spellingShingle Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
Ribeiro, Diego Luis
Antimutagenic
Flavonoids
Flow cytometry
Micronucleus
RT-qPCR
title_short Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
title_full Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
title_fullStr Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
title_full_unstemmed Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
title_sort Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro
author Ribeiro, Diego Luis
author_facet Ribeiro, Diego Luis
Cilião, Heloísa Lizotti
Specian, Ana Flávia Leal
Serpeloni, Juliana Mara [UNESP]
De Oliveira, Marcelo Tempesta
Varanda, Eliana Aparecida [UNESP]
Vilegas, Wagner [UNESP]
Saldanha, Luiz Leonardo [UNESP]
Martínez-López, Wilner
Dokkedal, Anne Lígia [UNESP]
Cólus, Ilce Mara Syllos
author_role author
author2 Cilião, Heloísa Lizotti
Specian, Ana Flávia Leal
Serpeloni, Juliana Mara [UNESP]
De Oliveira, Marcelo Tempesta
Varanda, Eliana Aparecida [UNESP]
Vilegas, Wagner [UNESP]
Saldanha, Luiz Leonardo [UNESP]
Martínez-López, Wilner
Dokkedal, Anne Lígia [UNESP]
Cólus, Ilce Mara Syllos
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Estadual Paulista (Unesp)
Institute of Biological Investigation Clemente Estable
dc.contributor.author.fl_str_mv Ribeiro, Diego Luis
Cilião, Heloísa Lizotti
Specian, Ana Flávia Leal
Serpeloni, Juliana Mara [UNESP]
De Oliveira, Marcelo Tempesta
Varanda, Eliana Aparecida [UNESP]
Vilegas, Wagner [UNESP]
Saldanha, Luiz Leonardo [UNESP]
Martínez-López, Wilner
Dokkedal, Anne Lígia [UNESP]
Cólus, Ilce Mara Syllos
dc.subject.por.fl_str_mv Antimutagenic
Flavonoids
Flow cytometry
Micronucleus
RT-qPCR
topic Antimutagenic
Flavonoids
Flow cytometry
Micronucleus
RT-qPCR
description Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 µg/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 µg/mL was chosen to evaluate the protective effects against DNA damage induced by benzo[a]pyrene (B[a]P). At concentrations higher than 7.5 µg/mL (between 10 and 50 µg/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B[a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:16:46Z
2018-12-11T17:16:46Z
2018-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10616-017-0173-5
Cytotechnology, v. 70, n. 2, p. 713-728, 2018.
1573-0778
0920-9069
http://hdl.handle.net/11449/175624
10.1007/s10616-017-0173-5
2-s2.0-85037717319
2-s2.0-85037717319.pdf
url http://dx.doi.org/10.1007/s10616-017-0173-5
http://hdl.handle.net/11449/175624
identifier_str_mv Cytotechnology, v. 70, n. 2, p. 713-728, 2018.
1573-0778
0920-9069
10.1007/s10616-017-0173-5
2-s2.0-85037717319
2-s2.0-85037717319.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cytotechnology
0,519
0,519
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 713-728
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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