Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/194460 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4249044J6 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?metodo=apresentar&id=K4794694D6 |
Resumo: | Copper nanoparticles can contaminate the aquatic environment, but their effects on fish and how they may differ from copper salts is not understood. Thus, in this work we compare the sublethal effects of copper nanoparticles (nCu) and copper chloride (Cu) on the freshwater teleost Prochilodus lineatus, known for its sensitivity to copper. Juveniles (n = 8/group) were exposed to 20 μg L−1 of copper as CuCl2 (Cu), 40 μg L−1 of copper nanoparticles (nCu), or only water (control), for 96 h. These concentrations were chosen to achieve similar dissolved copper concentration in both treatments (Cu: 10.29 ± 0.94 μg L−1; nCu: 12.16 ± 1.77 μg L−1). After the exposure, the following biological parameters were evaluated: copper accumulation in the gills, liver, gastrointestinal tract, kidney, and muscle; hematocrit (Ht) and hemoglobin content (Hb); branchial activity of Na+-K+-ATPase (NaKATP), H+-ATPase (HATP), Ca2+-ATPase (CaATP), and carbonic anhydrase (CA); glutathione content (GSH) and lipid peroxidation (LPO) in the liver; acetylcholinesterase activity (AChE) in the brain and muscle; and histopathology of the gills and liver. The gills of Cu-exposed fish were adversely affected, with increased copper content, inhibition of H+-ATPase and Ca2+-ATPase, and histological damage, including proliferation of mitochondria rich cells and/or mucous cells. In addition, LPO levels increased in the liver of Cu-exposed fish, indicating the occurrence of oxidative stress. Exposure to nCu promoted a decrease in Ht and Hb, indicating anemia, and an increase in branchial Na+-K+-ATPase and H+-ATPase activities, which can be an adaptive response to metabolic acidosis. Within the chosen biomarkers and the conditions tested, copper nanoparticles were less toxic than copper. However, the effects promoted by the nanoparticles were different from those promoted by copper. These results emphasize the need for a better understanding of copper nanoparticles toxicity in order to establish safe concentrations and avoid environment impacts. |
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Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approachBiomarkersNanotoxicologyOsmoregulationHematologyFishOxidative stressCopper nanoparticles can contaminate the aquatic environment, but their effects on fish and how they may differ from copper salts is not understood. Thus, in this work we compare the sublethal effects of copper nanoparticles (nCu) and copper chloride (Cu) on the freshwater teleost Prochilodus lineatus, known for its sensitivity to copper. Juveniles (n = 8/group) were exposed to 20 μg L−1 of copper as CuCl2 (Cu), 40 μg L−1 of copper nanoparticles (nCu), or only water (control), for 96 h. These concentrations were chosen to achieve similar dissolved copper concentration in both treatments (Cu: 10.29 ± 0.94 μg L−1; nCu: 12.16 ± 1.77 μg L−1). After the exposure, the following biological parameters were evaluated: copper accumulation in the gills, liver, gastrointestinal tract, kidney, and muscle; hematocrit (Ht) and hemoglobin content (Hb); branchial activity of Na+-K+-ATPase (NaKATP), H+-ATPase (HATP), Ca2+-ATPase (CaATP), and carbonic anhydrase (CA); glutathione content (GSH) and lipid peroxidation (LPO) in the liver; acetylcholinesterase activity (AChE) in the brain and muscle; and histopathology of the gills and liver. The gills of Cu-exposed fish were adversely affected, with increased copper content, inhibition of H+-ATPase and Ca2+-ATPase, and histological damage, including proliferation of mitochondria rich cells and/or mucous cells. In addition, LPO levels increased in the liver of Cu-exposed fish, indicating the occurrence of oxidative stress. Exposure to nCu promoted a decrease in Ht and Hb, indicating anemia, and an increase in branchial Na+-K+-ATPase and H+-ATPase activities, which can be an adaptive response to metabolic acidosis. Within the chosen biomarkers and the conditions tested, copper nanoparticles were less toxic than copper. However, the effects promoted by the nanoparticles were different from those promoted by copper. These results emphasize the need for a better understanding of copper nanoparticles toxicity in order to establish safe concentrations and avoid environment impacts.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PreprintUniversidade Estadual de Londrina – UEL, Londrina, Paraná, BrazilUniversidade Estadual Paulista – UNESP, Sorocaba, São Paulo, BrazilFAPESP: 2017/21004-5ElsevierUniversidade Estadual Paulista (Unesp)Tesser, Maria EduardaPaula, Angélica Alves deRisso, Wagner EzequielMonteiro, Renata AparecidaPereira, Anderson do Espirito SantoFraceto, Leonardo FernandesMartinez, Claudia Bueno dos Reis2020-12-01T19:47:03Z2020-12-01T19:47:03Z2020-02-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0048-9697http://hdl.handle.net/11449/19446010.1016/j.scitotenv.2019.135332http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4249044J6http://buscatextual.cnpq.br/buscatextual/visualizacv.do?metodo=apresentar&id=K4794694D60000-0002-4118-24910000-0002-2827-20380000-0003-1467-5140engScience of the total environmentinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-12-11T06:17:17Zoai:repositorio.unesp.br:11449/194460Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-11T06:17:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
title |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
spellingShingle |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach Tesser, Maria Eduarda Biomarkers Nanotoxicology Osmoregulation Hematology Fish Oxidative stress |
title_short |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
title_full |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
title_fullStr |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
title_full_unstemmed |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
title_sort |
Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: a comparative approach |
author |
Tesser, Maria Eduarda |
author_facet |
Tesser, Maria Eduarda Paula, Angélica Alves de Risso, Wagner Ezequiel Monteiro, Renata Aparecida Pereira, Anderson do Espirito Santo Fraceto, Leonardo Fernandes Martinez, Claudia Bueno dos Reis |
author_role |
author |
author2 |
Paula, Angélica Alves de Risso, Wagner Ezequiel Monteiro, Renata Aparecida Pereira, Anderson do Espirito Santo Fraceto, Leonardo Fernandes Martinez, Claudia Bueno dos Reis |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tesser, Maria Eduarda Paula, Angélica Alves de Risso, Wagner Ezequiel Monteiro, Renata Aparecida Pereira, Anderson do Espirito Santo Fraceto, Leonardo Fernandes Martinez, Claudia Bueno dos Reis |
dc.subject.por.fl_str_mv |
Biomarkers Nanotoxicology Osmoregulation Hematology Fish Oxidative stress |
topic |
Biomarkers Nanotoxicology Osmoregulation Hematology Fish Oxidative stress |
description |
Copper nanoparticles can contaminate the aquatic environment, but their effects on fish and how they may differ from copper salts is not understood. Thus, in this work we compare the sublethal effects of copper nanoparticles (nCu) and copper chloride (Cu) on the freshwater teleost Prochilodus lineatus, known for its sensitivity to copper. Juveniles (n = 8/group) were exposed to 20 μg L−1 of copper as CuCl2 (Cu), 40 μg L−1 of copper nanoparticles (nCu), or only water (control), for 96 h. These concentrations were chosen to achieve similar dissolved copper concentration in both treatments (Cu: 10.29 ± 0.94 μg L−1; nCu: 12.16 ± 1.77 μg L−1). After the exposure, the following biological parameters were evaluated: copper accumulation in the gills, liver, gastrointestinal tract, kidney, and muscle; hematocrit (Ht) and hemoglobin content (Hb); branchial activity of Na+-K+-ATPase (NaKATP), H+-ATPase (HATP), Ca2+-ATPase (CaATP), and carbonic anhydrase (CA); glutathione content (GSH) and lipid peroxidation (LPO) in the liver; acetylcholinesterase activity (AChE) in the brain and muscle; and histopathology of the gills and liver. The gills of Cu-exposed fish were adversely affected, with increased copper content, inhibition of H+-ATPase and Ca2+-ATPase, and histological damage, including proliferation of mitochondria rich cells and/or mucous cells. In addition, LPO levels increased in the liver of Cu-exposed fish, indicating the occurrence of oxidative stress. Exposure to nCu promoted a decrease in Ht and Hb, indicating anemia, and an increase in branchial Na+-K+-ATPase and H+-ATPase activities, which can be an adaptive response to metabolic acidosis. Within the chosen biomarkers and the conditions tested, copper nanoparticles were less toxic than copper. However, the effects promoted by the nanoparticles were different from those promoted by copper. These results emphasize the need for a better understanding of copper nanoparticles toxicity in order to establish safe concentrations and avoid environment impacts. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01T19:47:03Z 2020-12-01T19:47:03Z 2020-02-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
0048-9697 http://hdl.handle.net/11449/194460 10.1016/j.scitotenv.2019.135332 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4249044J6 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?metodo=apresentar&id=K4794694D6 0000-0002-4118-2491 0000-0002-2827-2038 0000-0003-1467-5140 |
identifier_str_mv |
0048-9697 10.1016/j.scitotenv.2019.135332 0000-0002-4118-2491 0000-0002-2827-2038 0000-0003-1467-5140 |
url |
http://hdl.handle.net/11449/194460 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4249044J6 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?metodo=apresentar&id=K4794694D6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Science of the total environment |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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