Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actbio.2018.06.008 http://hdl.handle.net/11449/176430 |
Resumo: | Secondary caries and hybrid layer degradation are two major challenges encountered in long-term resin-dentin bond stability. As a link between resin and dentin, adhesives that possess both antimicrobial and anti-proteolytic activities are in demand for eliminating bacteria-induced secondary caries and preventing hybrid layers from degradation. In the present study, a new quaternary ammonium methacryloxy silane (QAMS) prepared from sol-gel chemistry was incorporated into experimental adhesives to examine their antimicrobial effect and anti-proteolytic potential. This functional methacrylate resin monomer contains polymerizable methacryloxy functionalities as well as a positively-charged quaternary ammonium functionality with a long, lipophilic -C18H37 alkyl chain for puncturing the cell wall/membrane of surface-colonizing organisms. Antibacterial testing performed using agar diffusion test, live/dead bacterial staining and colony-forming unit counts all indicated that the QAMS-containing adhesives killed Streptococcus mutans and Actinomyces naeslundii in a dose-dependent manner via a predominant contact-killing mechanism. Gelatinolytic activity within the hybrid layers created by these adhesives was examined using in-situ zymography. Hybrid layers created with 0% QAMS-containing adhesive exhibited intense green fluorescence emitted by the hydrolyzed fluorescein-conjugated gelatin, with 4-fold increase in enzymatic activity compared with an experimental adhesive containing 5% QAMS. Taken together, incorporation of 5% QAMS in the experimental adhesive provides simultaneous antimicrobial and anti-proteolytic activities that are crucial for the maintenance of long-term resin-dentin bond integrity. Statement of Significance: Durability of resin-dentin interfacial bond remains a clinically-significant challenge. Secondary caries caused by bacteria and the degradation of hybrid layers via endogenous dentin proteases are two important contributors to the poor resin-dentin bond durability. The present study developed a new 5% QAMS-containing adhesive that provides simultaneous antimicrobial and dentin protease inhibition functions to extend the longevity of resin-dentin bonds. |
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Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activitiesAntibacterialEndogenous dentin proteasesQuaternary ammonium methacryloxy silaneResin-dentin bondsSecondary caries and hybrid layer degradation are two major challenges encountered in long-term resin-dentin bond stability. As a link between resin and dentin, adhesives that possess both antimicrobial and anti-proteolytic activities are in demand for eliminating bacteria-induced secondary caries and preventing hybrid layers from degradation. In the present study, a new quaternary ammonium methacryloxy silane (QAMS) prepared from sol-gel chemistry was incorporated into experimental adhesives to examine their antimicrobial effect and anti-proteolytic potential. This functional methacrylate resin monomer contains polymerizable methacryloxy functionalities as well as a positively-charged quaternary ammonium functionality with a long, lipophilic -C18H37 alkyl chain for puncturing the cell wall/membrane of surface-colonizing organisms. Antibacterial testing performed using agar diffusion test, live/dead bacterial staining and colony-forming unit counts all indicated that the QAMS-containing adhesives killed Streptococcus mutans and Actinomyces naeslundii in a dose-dependent manner via a predominant contact-killing mechanism. Gelatinolytic activity within the hybrid layers created by these adhesives was examined using in-situ zymography. Hybrid layers created with 0% QAMS-containing adhesive exhibited intense green fluorescence emitted by the hydrolyzed fluorescein-conjugated gelatin, with 4-fold increase in enzymatic activity compared with an experimental adhesive containing 5% QAMS. Taken together, incorporation of 5% QAMS in the experimental adhesive provides simultaneous antimicrobial and anti-proteolytic activities that are crucial for the maintenance of long-term resin-dentin bond integrity. Statement of Significance: Durability of resin-dentin interfacial bond remains a clinically-significant challenge. Secondary caries caused by bacteria and the degradation of hybrid layers via endogenous dentin proteases are two important contributors to the poor resin-dentin bond durability. The present study developed a new 5% QAMS-containing adhesive that provides simultaneous antimicrobial and dentin protease inhibition functions to extend the longevity of resin-dentin bonds.National Natural Science Foundation of ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics West China Hospital of Stomatology Sichuan UniversityThe Dental College of Georgia Augusta UniversityDepartment of Restorative Dentistry Institute of Science and Technology São Paulo State University UNESP São Jose dos CamposDepartment of Biomedical and Neuromotor Sciences DIBINEM University of Bologna – Alma Mater StudiorumState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases School of Stomatology The Fourth Military Medical UniversityDepartment of Restorative Dentistry Institute of Science and Technology São Paulo State University UNESP São Jose dos CamposSichuan UniversityAugusta UniversityUniversidade Estadual Paulista (Unesp)University of Bologna – Alma Mater StudiorumThe Fourth Military Medical UniversityGou, Ya-pingMeghil, Mohamed M.Pucci, Cesar R. [UNESP]Breschi, LorenzoPashley, David H.Cutler, Christopher W.Niu, Li-naLi, Ji-yaoTay, Franklin R.2018-12-11T17:20:46Z2018-12-11T17:20:46Z2018-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article171-182application/pdfhttp://dx.doi.org/10.1016/j.actbio.2018.06.008Acta Biomaterialia, v. 75, p. 171-182.1878-75681742-7061http://hdl.handle.net/11449/17643010.1016/j.actbio.2018.06.0082-s2.0-850483229512-s2.0-85048322951.pdf17540206528748500000-0003-4830-0400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Biomaterialia1,967info:eu-repo/semantics/openAccess2023-10-12T06:03:17Zoai:repositorio.unesp.br:11449/176430Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:39:47.125881Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
title |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
spellingShingle |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities Gou, Ya-ping Antibacterial Endogenous dentin proteases Quaternary ammonium methacryloxy silane Resin-dentin bonds |
title_short |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
title_full |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
title_fullStr |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
title_full_unstemmed |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
title_sort |
Optimizing resin-dentin bond stability using a bioactive adhesive with concomitant antibacterial properties and anti-proteolytic activities |
author |
Gou, Ya-ping |
author_facet |
Gou, Ya-ping Meghil, Mohamed M. Pucci, Cesar R. [UNESP] Breschi, Lorenzo Pashley, David H. Cutler, Christopher W. Niu, Li-na Li, Ji-yao Tay, Franklin R. |
author_role |
author |
author2 |
Meghil, Mohamed M. Pucci, Cesar R. [UNESP] Breschi, Lorenzo Pashley, David H. Cutler, Christopher W. Niu, Li-na Li, Ji-yao Tay, Franklin R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sichuan University Augusta University Universidade Estadual Paulista (Unesp) University of Bologna – Alma Mater Studiorum The Fourth Military Medical University |
dc.contributor.author.fl_str_mv |
Gou, Ya-ping Meghil, Mohamed M. Pucci, Cesar R. [UNESP] Breschi, Lorenzo Pashley, David H. Cutler, Christopher W. Niu, Li-na Li, Ji-yao Tay, Franklin R. |
dc.subject.por.fl_str_mv |
Antibacterial Endogenous dentin proteases Quaternary ammonium methacryloxy silane Resin-dentin bonds |
topic |
Antibacterial Endogenous dentin proteases Quaternary ammonium methacryloxy silane Resin-dentin bonds |
description |
Secondary caries and hybrid layer degradation are two major challenges encountered in long-term resin-dentin bond stability. As a link between resin and dentin, adhesives that possess both antimicrobial and anti-proteolytic activities are in demand for eliminating bacteria-induced secondary caries and preventing hybrid layers from degradation. In the present study, a new quaternary ammonium methacryloxy silane (QAMS) prepared from sol-gel chemistry was incorporated into experimental adhesives to examine their antimicrobial effect and anti-proteolytic potential. This functional methacrylate resin monomer contains polymerizable methacryloxy functionalities as well as a positively-charged quaternary ammonium functionality with a long, lipophilic -C18H37 alkyl chain for puncturing the cell wall/membrane of surface-colonizing organisms. Antibacterial testing performed using agar diffusion test, live/dead bacterial staining and colony-forming unit counts all indicated that the QAMS-containing adhesives killed Streptococcus mutans and Actinomyces naeslundii in a dose-dependent manner via a predominant contact-killing mechanism. Gelatinolytic activity within the hybrid layers created by these adhesives was examined using in-situ zymography. Hybrid layers created with 0% QAMS-containing adhesive exhibited intense green fluorescence emitted by the hydrolyzed fluorescein-conjugated gelatin, with 4-fold increase in enzymatic activity compared with an experimental adhesive containing 5% QAMS. Taken together, incorporation of 5% QAMS in the experimental adhesive provides simultaneous antimicrobial and anti-proteolytic activities that are crucial for the maintenance of long-term resin-dentin bond integrity. Statement of Significance: Durability of resin-dentin interfacial bond remains a clinically-significant challenge. Secondary caries caused by bacteria and the degradation of hybrid layers via endogenous dentin proteases are two important contributors to the poor resin-dentin bond durability. The present study developed a new 5% QAMS-containing adhesive that provides simultaneous antimicrobial and dentin protease inhibition functions to extend the longevity of resin-dentin bonds. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:20:46Z 2018-12-11T17:20:46Z 2018-07-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actbio.2018.06.008 Acta Biomaterialia, v. 75, p. 171-182. 1878-7568 1742-7061 http://hdl.handle.net/11449/176430 10.1016/j.actbio.2018.06.008 2-s2.0-85048322951 2-s2.0-85048322951.pdf 1754020652874850 0000-0003-4830-0400 |
url |
http://dx.doi.org/10.1016/j.actbio.2018.06.008 http://hdl.handle.net/11449/176430 |
identifier_str_mv |
Acta Biomaterialia, v. 75, p. 171-182. 1878-7568 1742-7061 10.1016/j.actbio.2018.06.008 2-s2.0-85048322951 2-s2.0-85048322951.pdf 1754020652874850 0000-0003-4830-0400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Biomaterialia 1,967 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
171-182 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128397268746240 |