Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-84551997000100015 http://hdl.handle.net/11449/65056 |
Resumo: | Several studies have demonstrated that lymphocytes from patients with Down syndrome (DS) exhibit an increased frequency of chromosome aberrations when they are exposed to ionizing radiation or to chemicals at the G0 or G1 phases of the cell cycle, but not at G2 when compared to normal subjects. To determine the susceptibility of DS lymphocytes at G2 phase, bleomycin, a radiomimetic agent, was used to induce DNA breaks in blood cultures from 24 Down syndrome patients. All the patients with DS showed free trisomy 21 (47,XX + 21 or 47,XY + 21). Individuals that showed an average number of chromatid breaks per cell higher than 0.8 were considered sensitive to the drug. No control child showed susceptibility to bleomycin, and among the 24 patients with DS, only one was sensitive to the drug. No significant difference was observed between the two groups, regarding chromatid break frequencies in treated G2 lymphocytes. The distribution of bleomycin-induced breaks in each group of chromosomes was similar for DS and controls. No significant difference was found in the response to bleomycin between male and female subjects. Probably, the main factor involved in chromosome sensitivity of lymphocytes from patients with DS is the phase of the cell cycle in which the cell is treated. |
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Repositório Institucional da UNESP |
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Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patientsbleomycinbleomycin sulfateantibiotic sensitivityblood culturecell cycle g2 phasechildchromatidchromosome aberrationcontrolled studydna strand breakagedown syndromefemalehumanhuman cellinfantionizing radiationlymphocyte culturemaletrisomy 21Several studies have demonstrated that lymphocytes from patients with Down syndrome (DS) exhibit an increased frequency of chromosome aberrations when they are exposed to ionizing radiation or to chemicals at the G0 or G1 phases of the cell cycle, but not at G2 when compared to normal subjects. To determine the susceptibility of DS lymphocytes at G2 phase, bleomycin, a radiomimetic agent, was used to induce DNA breaks in blood cultures from 24 Down syndrome patients. All the patients with DS showed free trisomy 21 (47,XX + 21 or 47,XY + 21). Individuals that showed an average number of chromatid breaks per cell higher than 0.8 were considered sensitive to the drug. No control child showed susceptibility to bleomycin, and among the 24 patients with DS, only one was sensitive to the drug. No significant difference was observed between the two groups, regarding chromatid break frequencies in treated G2 lymphocytes. The distribution of bleomycin-induced breaks in each group of chromosomes was similar for DS and controls. No significant difference was found in the response to bleomycin between male and female subjects. Probably, the main factor involved in chromosome sensitivity of lymphocytes from patients with DS is the phase of the cell cycle in which the cell is treated.Departamento de Genética Instituto de Biociências Universidade Estadual Paulista, 18618-000 Botucatu, SPDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista, 18618-000 Botucatu, SPUniversidade Estadual Paulista (Unesp)Bartholomei-Santos, Marlise Ladvocat [UNESP]De Lucca, Edmundo José [UNESP]2014-05-27T11:18:12Z2014-05-27T11:18:12Z1997-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article79-85http://dx.doi.org/10.1590/S0100-84551997000100015Brazilian Journal of Genetics, v. 20, n. 1, p. 79-85, 1997.0100-8455http://hdl.handle.net/11449/6505610.1590/S0100-84551997000100015S0100-84551997000100015WOS:A1997WT019000152-s2.0-0030914601Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Geneticsinfo:eu-repo/semantics/openAccess2021-10-23T07:40:25Zoai:repositorio.unesp.br:11449/65056Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:26:34.391748Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
title |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
spellingShingle |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients Bartholomei-Santos, Marlise Ladvocat [UNESP] bleomycin bleomycin sulfate antibiotic sensitivity blood culture cell cycle g2 phase child chromatid chromosome aberration controlled study dna strand breakage down syndrome female human human cell infant ionizing radiation lymphocyte culture male trisomy 21 |
title_short |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
title_full |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
title_fullStr |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
title_full_unstemmed |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
title_sort |
Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients |
author |
Bartholomei-Santos, Marlise Ladvocat [UNESP] |
author_facet |
Bartholomei-Santos, Marlise Ladvocat [UNESP] De Lucca, Edmundo José [UNESP] |
author_role |
author |
author2 |
De Lucca, Edmundo José [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bartholomei-Santos, Marlise Ladvocat [UNESP] De Lucca, Edmundo José [UNESP] |
dc.subject.por.fl_str_mv |
bleomycin bleomycin sulfate antibiotic sensitivity blood culture cell cycle g2 phase child chromatid chromosome aberration controlled study dna strand breakage down syndrome female human human cell infant ionizing radiation lymphocyte culture male trisomy 21 |
topic |
bleomycin bleomycin sulfate antibiotic sensitivity blood culture cell cycle g2 phase child chromatid chromosome aberration controlled study dna strand breakage down syndrome female human human cell infant ionizing radiation lymphocyte culture male trisomy 21 |
description |
Several studies have demonstrated that lymphocytes from patients with Down syndrome (DS) exhibit an increased frequency of chromosome aberrations when they are exposed to ionizing radiation or to chemicals at the G0 or G1 phases of the cell cycle, but not at G2 when compared to normal subjects. To determine the susceptibility of DS lymphocytes at G2 phase, bleomycin, a radiomimetic agent, was used to induce DNA breaks in blood cultures from 24 Down syndrome patients. All the patients with DS showed free trisomy 21 (47,XX + 21 or 47,XY + 21). Individuals that showed an average number of chromatid breaks per cell higher than 0.8 were considered sensitive to the drug. No control child showed susceptibility to bleomycin, and among the 24 patients with DS, only one was sensitive to the drug. No significant difference was observed between the two groups, regarding chromatid break frequencies in treated G2 lymphocytes. The distribution of bleomycin-induced breaks in each group of chromosomes was similar for DS and controls. No significant difference was found in the response to bleomycin between male and female subjects. Probably, the main factor involved in chromosome sensitivity of lymphocytes from patients with DS is the phase of the cell cycle in which the cell is treated. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-03-01 2014-05-27T11:18:12Z 2014-05-27T11:18:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-84551997000100015 Brazilian Journal of Genetics, v. 20, n. 1, p. 79-85, 1997. 0100-8455 http://hdl.handle.net/11449/65056 10.1590/S0100-84551997000100015 S0100-84551997000100015 WOS:A1997WT01900015 2-s2.0-0030914601 |
url |
http://dx.doi.org/10.1590/S0100-84551997000100015 http://hdl.handle.net/11449/65056 |
identifier_str_mv |
Brazilian Journal of Genetics, v. 20, n. 1, p. 79-85, 1997. 0100-8455 10.1590/S0100-84551997000100015 S0100-84551997000100015 WOS:A1997WT01900015 2-s2.0-0030914601 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
79-85 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128812891766784 |