A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead

Detalhes bibliográficos
Autor(a) principal: Trama-Freitas, Bianca [UNESP]
Data de Publicação: 2017
Outros Autores: Freitas, Johnattan C.S. [UNESP], Martins, Rui C., Gando-Ferreira, Licínio M., Quinta-Ferreira, Maria Emília, Quinta-Ferreira, Rosa Maria, do Carmo, Devaney R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jbiotec.2017.10.015
http://hdl.handle.net/11449/175395
Resumo: Lead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages.
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spelling A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of leadAutofluorescenceBiosorptionLeadNeuronal toxicitySilsesquioxaneToxicityYeastLead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Engenharia de Ilha Solteira Univ. Estadual Paulista-UNESP Departamento de Física e Química, 56 CEP, Av. Brasil CentroCIEPQPF- Research Center of Chemical Process Engineering and Forest Products Department of Chemical Engineering University of CoimbraCNC- Center for Neuroscience and Cell Biology and Department of Physics University of CoimbraFaculdade de Engenharia de Ilha Solteira Univ. Estadual Paulista-UNESP Departamento de Física e Química, 56 CEP, Av. Brasil CentroUniversidade Estadual Paulista (Unesp)University of CoimbraTrama-Freitas, Bianca [UNESP]Freitas, Johnattan C.S. [UNESP]Martins, Rui C.Gando-Ferreira, Licínio M.Quinta-Ferreira, Maria EmíliaQuinta-Ferreira, Rosa Mariado Carmo, Devaney R. [UNESP]2018-12-11T17:15:38Z2018-12-11T17:15:38Z2017-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-50application/pdfhttp://dx.doi.org/10.1016/j.jbiotec.2017.10.015Journal of Biotechnology, v. 264, p. 43-50.1873-48630168-1656http://hdl.handle.net/11449/17539510.1016/j.jbiotec.2017.10.0152-s2.0-850323796022-s2.0-85032379602.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biotechnology0,929info:eu-repo/semantics/openAccess2024-07-10T14:08:18Zoai:repositorio.unesp.br:11449/175395Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:47.126595Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
title A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
spellingShingle A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
Trama-Freitas, Bianca [UNESP]
Autofluorescence
Biosorption
Lead
Neuronal toxicity
Silsesquioxane
Toxicity
Yeast
title_short A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
title_full A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
title_fullStr A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
title_full_unstemmed A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
title_sort A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
author Trama-Freitas, Bianca [UNESP]
author_facet Trama-Freitas, Bianca [UNESP]
Freitas, Johnattan C.S. [UNESP]
Martins, Rui C.
Gando-Ferreira, Licínio M.
Quinta-Ferreira, Maria Emília
Quinta-Ferreira, Rosa Maria
do Carmo, Devaney R. [UNESP]
author_role author
author2 Freitas, Johnattan C.S. [UNESP]
Martins, Rui C.
Gando-Ferreira, Licínio M.
Quinta-Ferreira, Maria Emília
Quinta-Ferreira, Rosa Maria
do Carmo, Devaney R. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Coimbra
dc.contributor.author.fl_str_mv Trama-Freitas, Bianca [UNESP]
Freitas, Johnattan C.S. [UNESP]
Martins, Rui C.
Gando-Ferreira, Licínio M.
Quinta-Ferreira, Maria Emília
Quinta-Ferreira, Rosa Maria
do Carmo, Devaney R. [UNESP]
dc.subject.por.fl_str_mv Autofluorescence
Biosorption
Lead
Neuronal toxicity
Silsesquioxane
Toxicity
Yeast
topic Autofluorescence
Biosorption
Lead
Neuronal toxicity
Silsesquioxane
Toxicity
Yeast
description Lead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-20
2018-12-11T17:15:38Z
2018-12-11T17:15:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jbiotec.2017.10.015
Journal of Biotechnology, v. 264, p. 43-50.
1873-4863
0168-1656
http://hdl.handle.net/11449/175395
10.1016/j.jbiotec.2017.10.015
2-s2.0-85032379602
2-s2.0-85032379602.pdf
url http://dx.doi.org/10.1016/j.jbiotec.2017.10.015
http://hdl.handle.net/11449/175395
identifier_str_mv Journal of Biotechnology, v. 264, p. 43-50.
1873-4863
0168-1656
10.1016/j.jbiotec.2017.10.015
2-s2.0-85032379602
2-s2.0-85032379602.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Biotechnology
0,929
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 43-50
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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