A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jbiotec.2017.10.015 http://hdl.handle.net/11449/175395 |
Resumo: | Lead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages. |
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A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of leadAutofluorescenceBiosorptionLeadNeuronal toxicitySilsesquioxaneToxicityYeastLead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Engenharia de Ilha Solteira Univ. Estadual Paulista-UNESP Departamento de Física e Química, 56 CEP, Av. Brasil CentroCIEPQPF- Research Center of Chemical Process Engineering and Forest Products Department of Chemical Engineering University of CoimbraCNC- Center for Neuroscience and Cell Biology and Department of Physics University of CoimbraFaculdade de Engenharia de Ilha Solteira Univ. Estadual Paulista-UNESP Departamento de Física e Química, 56 CEP, Av. Brasil CentroUniversidade Estadual Paulista (Unesp)University of CoimbraTrama-Freitas, Bianca [UNESP]Freitas, Johnattan C.S. [UNESP]Martins, Rui C.Gando-Ferreira, Licínio M.Quinta-Ferreira, Maria EmíliaQuinta-Ferreira, Rosa Mariado Carmo, Devaney R. [UNESP]2018-12-11T17:15:38Z2018-12-11T17:15:38Z2017-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-50application/pdfhttp://dx.doi.org/10.1016/j.jbiotec.2017.10.015Journal of Biotechnology, v. 264, p. 43-50.1873-48630168-1656http://hdl.handle.net/11449/17539510.1016/j.jbiotec.2017.10.0152-s2.0-850323796022-s2.0-85032379602.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biotechnology0,929info:eu-repo/semantics/openAccess2024-07-10T14:08:18Zoai:repositorio.unesp.br:11449/175395Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:47.126595Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
title |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
spellingShingle |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead Trama-Freitas, Bianca [UNESP] Autofluorescence Biosorption Lead Neuronal toxicity Silsesquioxane Toxicity Yeast |
title_short |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
title_full |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
title_fullStr |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
title_full_unstemmed |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
title_sort |
A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead |
author |
Trama-Freitas, Bianca [UNESP] |
author_facet |
Trama-Freitas, Bianca [UNESP] Freitas, Johnattan C.S. [UNESP] Martins, Rui C. Gando-Ferreira, Licínio M. Quinta-Ferreira, Maria Emília Quinta-Ferreira, Rosa Maria do Carmo, Devaney R. [UNESP] |
author_role |
author |
author2 |
Freitas, Johnattan C.S. [UNESP] Martins, Rui C. Gando-Ferreira, Licínio M. Quinta-Ferreira, Maria Emília Quinta-Ferreira, Rosa Maria do Carmo, Devaney R. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Coimbra |
dc.contributor.author.fl_str_mv |
Trama-Freitas, Bianca [UNESP] Freitas, Johnattan C.S. [UNESP] Martins, Rui C. Gando-Ferreira, Licínio M. Quinta-Ferreira, Maria Emília Quinta-Ferreira, Rosa Maria do Carmo, Devaney R. [UNESP] |
dc.subject.por.fl_str_mv |
Autofluorescence Biosorption Lead Neuronal toxicity Silsesquioxane Toxicity Yeast |
topic |
Autofluorescence Biosorption Lead Neuronal toxicity Silsesquioxane Toxicity Yeast |
description |
Lead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-20 2018-12-11T17:15:38Z 2018-12-11T17:15:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jbiotec.2017.10.015 Journal of Biotechnology, v. 264, p. 43-50. 1873-4863 0168-1656 http://hdl.handle.net/11449/175395 10.1016/j.jbiotec.2017.10.015 2-s2.0-85032379602 2-s2.0-85032379602.pdf |
url |
http://dx.doi.org/10.1016/j.jbiotec.2017.10.015 http://hdl.handle.net/11449/175395 |
identifier_str_mv |
Journal of Biotechnology, v. 264, p. 43-50. 1873-4863 0168-1656 10.1016/j.jbiotec.2017.10.015 2-s2.0-85032379602 2-s2.0-85032379602.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biotechnology 0,929 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
43-50 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129584505290752 |