Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/biolre/ioab149 http://hdl.handle.net/11449/231579 |
Resumo: | In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus. |
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Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifersbeef heifersperi-estrus perioduterine luminal fluid metabolomeIn cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus.Department of Animal Sciences University of Florida FLDepartment of Animal Reproduction School of Veterinary Medicine and Animal Sciences University of São PauloDepartment of Surgery Division of Urology Federal University of São PauloSchool of Veterinary Medicine São Paulo State UniversitySchool of Veterinary Medicine São Paulo State UniversityFLUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Silva, Felipe A C Cda Silva, Gabriela F.Vieira, Bruna S.Neto, Adomar L.Rocha, Cecilia C.Lo Turco, Edson G.Nogueira, Guilherme P. [UNESP]Pugliesi, GuilhermeBinelli, Mario2022-04-29T08:46:14Z2022-04-29T08:46:14Z2021-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1140-1153http://dx.doi.org/10.1093/biolre/ioab149Biology of reproduction, v. 105, n. 5, p. 1140-1153, 2021.1529-7268http://hdl.handle.net/11449/23157910.1093/biolre/ioab1492-s2.0-85121967258Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiology of reproductioninfo:eu-repo/semantics/openAccess2024-09-09T14:06:18Zoai:repositorio.unesp.br:11449/231579Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
title |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
spellingShingle |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers Silva, Felipe A C C beef heifers peri-estrus period uterine luminal fluid metabolome |
title_short |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
title_full |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
title_fullStr |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
title_full_unstemmed |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
title_sort |
Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers |
author |
Silva, Felipe A C C |
author_facet |
Silva, Felipe A C C da Silva, Gabriela F. Vieira, Bruna S. Neto, Adomar L. Rocha, Cecilia C. Lo Turco, Edson G. Nogueira, Guilherme P. [UNESP] Pugliesi, Guilherme Binelli, Mario |
author_role |
author |
author2 |
da Silva, Gabriela F. Vieira, Bruna S. Neto, Adomar L. Rocha, Cecilia C. Lo Turco, Edson G. Nogueira, Guilherme P. [UNESP] Pugliesi, Guilherme Binelli, Mario |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
FL Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, Felipe A C C da Silva, Gabriela F. Vieira, Bruna S. Neto, Adomar L. Rocha, Cecilia C. Lo Turco, Edson G. Nogueira, Guilherme P. [UNESP] Pugliesi, Guilherme Binelli, Mario |
dc.subject.por.fl_str_mv |
beef heifers peri-estrus period uterine luminal fluid metabolome |
topic |
beef heifers peri-estrus period uterine luminal fluid metabolome |
description |
In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-15 2022-04-29T08:46:14Z 2022-04-29T08:46:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/biolre/ioab149 Biology of reproduction, v. 105, n. 5, p. 1140-1153, 2021. 1529-7268 http://hdl.handle.net/11449/231579 10.1093/biolre/ioab149 2-s2.0-85121967258 |
url |
http://dx.doi.org/10.1093/biolre/ioab149 http://hdl.handle.net/11449/231579 |
identifier_str_mv |
Biology of reproduction, v. 105, n. 5, p. 1140-1153, 2021. 1529-7268 10.1093/biolre/ioab149 2-s2.0-85121967258 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biology of reproduction |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1140-1153 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546619471134720 |