Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers

Detalhes bibliográficos
Autor(a) principal: Silva, Felipe A C C
Data de Publicação: 2021
Outros Autores: da Silva, Gabriela F., Vieira, Bruna S., Neto, Adomar L., Rocha, Cecilia C., Lo Turco, Edson G., Nogueira, Guilherme P. [UNESP], Pugliesi, Guilherme, Binelli, Mario
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1093/biolre/ioab149
http://hdl.handle.net/11449/231579
Resumo: In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus.
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spelling Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifersbeef heifersperi-estrus perioduterine luminal fluid metabolomeIn cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus.Department of Animal Sciences University of Florida FLDepartment of Animal Reproduction School of Veterinary Medicine and Animal Sciences University of São PauloDepartment of Surgery Division of Urology Federal University of São PauloSchool of Veterinary Medicine São Paulo State UniversitySchool of Veterinary Medicine São Paulo State UniversityFLUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Silva, Felipe A C Cda Silva, Gabriela F.Vieira, Bruna S.Neto, Adomar L.Rocha, Cecilia C.Lo Turco, Edson G.Nogueira, Guilherme P. [UNESP]Pugliesi, GuilhermeBinelli, Mario2022-04-29T08:46:14Z2022-04-29T08:46:14Z2021-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1140-1153http://dx.doi.org/10.1093/biolre/ioab149Biology of reproduction, v. 105, n. 5, p. 1140-1153, 2021.1529-7268http://hdl.handle.net/11449/23157910.1093/biolre/ioab1492-s2.0-85121967258Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiology of reproductioninfo:eu-repo/semantics/openAccess2024-09-09T14:06:18Zoai:repositorio.unesp.br:11449/231579Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
title Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
spellingShingle Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
Silva, Felipe A C C
beef heifers
peri-estrus period
uterine luminal fluid metabolome
title_short Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
title_full Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
title_fullStr Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
title_full_unstemmed Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
title_sort Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers
author Silva, Felipe A C C
author_facet Silva, Felipe A C C
da Silva, Gabriela F.
Vieira, Bruna S.
Neto, Adomar L.
Rocha, Cecilia C.
Lo Turco, Edson G.
Nogueira, Guilherme P. [UNESP]
Pugliesi, Guilherme
Binelli, Mario
author_role author
author2 da Silva, Gabriela F.
Vieira, Bruna S.
Neto, Adomar L.
Rocha, Cecilia C.
Lo Turco, Edson G.
Nogueira, Guilherme P. [UNESP]
Pugliesi, Guilherme
Binelli, Mario
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv FL
Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Silva, Felipe A C C
da Silva, Gabriela F.
Vieira, Bruna S.
Neto, Adomar L.
Rocha, Cecilia C.
Lo Turco, Edson G.
Nogueira, Guilherme P. [UNESP]
Pugliesi, Guilherme
Binelli, Mario
dc.subject.por.fl_str_mv beef heifers
peri-estrus period
uterine luminal fluid metabolome
topic beef heifers
peri-estrus period
uterine luminal fluid metabolome
description In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-15
2022-04-29T08:46:14Z
2022-04-29T08:46:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/biolre/ioab149
Biology of reproduction, v. 105, n. 5, p. 1140-1153, 2021.
1529-7268
http://hdl.handle.net/11449/231579
10.1093/biolre/ioab149
2-s2.0-85121967258
url http://dx.doi.org/10.1093/biolre/ioab149
http://hdl.handle.net/11449/231579
identifier_str_mv Biology of reproduction, v. 105, n. 5, p. 1140-1153, 2021.
1529-7268
10.1093/biolre/ioab149
2-s2.0-85121967258
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biology of reproduction
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1140-1153
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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