Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/CompCytogen.v14i1.46852 http://hdl.handle.net/11449/196653 |
Resumo: | South American frogs of the genus Eupsophus Fitzinger, 1843 comprise 10 species. Two of them, Eupsophus vertebralis Grandison, 1961 and E. emiliopugini Formas, 1989 belong to the Eupsophus vertebralis group, exhibiting 2n = 28. Fundamental number differences between these species have been described using conventional chromosome staining of few specimens from only two localities. Here, classical techniques (Giemsa, C-handing, CMA(3)/DAPI handing, and Ag-NOR staining), and fluorescence in situ hybridization (FISH, with telomeric and 28S ribosomal probes), were applied on individuals of both species collected from 15 localities. We corroborate differences in fundamental numbers (FN) between E. vertebralis and E. emiliopugini through Giemsa staining and C-banding (FN = 54 and 56, respectively). No interstitial fluorescent signals, but clearly stained telomeric regions were detected by FISH using telomeric probe over spreads from both species. FISH with 28S rDNA probes and Ag-NOR staining confirmed the active nucleolus organizer regions signal on pair 5 for both species. Nevertheless, one E. emiliopugini individual from the Puyehue locality exhibited 28S ribosomal signals on pairs 4 and 5. Interestingly, only one chromosome of each pair showed Ag-NOR positive signals, showing a nucleolar dominance pattern. Chromosomal rearrangements, rRNA gene dosage control, mobile NORs elements, and/or species hybridization process could he involved in this interpopulation chromosomal variation. |
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Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiationKaryotype variationsFISHPatagonian frogsribosomal probeNOR polymorphismSouth American frogs of the genus Eupsophus Fitzinger, 1843 comprise 10 species. Two of them, Eupsophus vertebralis Grandison, 1961 and E. emiliopugini Formas, 1989 belong to the Eupsophus vertebralis group, exhibiting 2n = 28. Fundamental number differences between these species have been described using conventional chromosome staining of few specimens from only two localities. Here, classical techniques (Giemsa, C-handing, CMA(3)/DAPI handing, and Ag-NOR staining), and fluorescence in situ hybridization (FISH, with telomeric and 28S ribosomal probes), were applied on individuals of both species collected from 15 localities. We corroborate differences in fundamental numbers (FN) between E. vertebralis and E. emiliopugini through Giemsa staining and C-banding (FN = 54 and 56, respectively). No interstitial fluorescent signals, but clearly stained telomeric regions were detected by FISH using telomeric probe over spreads from both species. FISH with 28S rDNA probes and Ag-NOR staining confirmed the active nucleolus organizer regions signal on pair 5 for both species. Nevertheless, one E. emiliopugini individual from the Puyehue locality exhibited 28S ribosomal signals on pairs 4 and 5. Interestingly, only one chromosome of each pair showed Ag-NOR positive signals, showing a nucleolar dominance pattern. Chromosomal rearrangements, rRNA gene dosage control, mobile NORs elements, and/or species hybridization process could he involved in this interpopulation chromosomal variation.FondecytCONICYTUniv Austral Chile, Inst Ciencias Marinas & Limnol, Edificio Emilio Pugin,Campus Isla Teja S-N, Valdivia, ChileUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilFondecyt: 3160328CONICYT: 22180766Pensoft PublishersUniv Austral ChileUniversidade Estadual Paulista (Unesp)Quercia, Camila A.Suarez-Villota, Elkin Y.Foresti, Fausto [UNESP]Nunez, Jose J.2020-12-10T19:51:53Z2020-12-10T19:51:53Z2020-01-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article61-74http://dx.doi.org/10.3897/CompCytogen.v14i1.46852Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 14, n. 1, p. 61-74, 2020.1993-0771http://hdl.handle.net/11449/19665310.3897/CompCytogen.v14i1.46852WOS:000518398400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogeneticsinfo:eu-repo/semantics/openAccess2021-10-23T08:59:40Zoai:repositorio.unesp.br:11449/196653Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:59:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
title |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
spellingShingle |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation Quercia, Camila A. Karyotype variations FISH Patagonian frogs ribosomal probe NOR polymorphism |
title_short |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
title_full |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
title_fullStr |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
title_full_unstemmed |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
title_sort |
Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation |
author |
Quercia, Camila A. |
author_facet |
Quercia, Camila A. Suarez-Villota, Elkin Y. Foresti, Fausto [UNESP] Nunez, Jose J. |
author_role |
author |
author2 |
Suarez-Villota, Elkin Y. Foresti, Fausto [UNESP] Nunez, Jose J. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Austral Chile Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Quercia, Camila A. Suarez-Villota, Elkin Y. Foresti, Fausto [UNESP] Nunez, Jose J. |
dc.subject.por.fl_str_mv |
Karyotype variations FISH Patagonian frogs ribosomal probe NOR polymorphism |
topic |
Karyotype variations FISH Patagonian frogs ribosomal probe NOR polymorphism |
description |
South American frogs of the genus Eupsophus Fitzinger, 1843 comprise 10 species. Two of them, Eupsophus vertebralis Grandison, 1961 and E. emiliopugini Formas, 1989 belong to the Eupsophus vertebralis group, exhibiting 2n = 28. Fundamental number differences between these species have been described using conventional chromosome staining of few specimens from only two localities. Here, classical techniques (Giemsa, C-handing, CMA(3)/DAPI handing, and Ag-NOR staining), and fluorescence in situ hybridization (FISH, with telomeric and 28S ribosomal probes), were applied on individuals of both species collected from 15 localities. We corroborate differences in fundamental numbers (FN) between E. vertebralis and E. emiliopugini through Giemsa staining and C-banding (FN = 54 and 56, respectively). No interstitial fluorescent signals, but clearly stained telomeric regions were detected by FISH using telomeric probe over spreads from both species. FISH with 28S rDNA probes and Ag-NOR staining confirmed the active nucleolus organizer regions signal on pair 5 for both species. Nevertheless, one E. emiliopugini individual from the Puyehue locality exhibited 28S ribosomal signals on pairs 4 and 5. Interestingly, only one chromosome of each pair showed Ag-NOR positive signals, showing a nucleolar dominance pattern. Chromosomal rearrangements, rRNA gene dosage control, mobile NORs elements, and/or species hybridization process could he involved in this interpopulation chromosomal variation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:51:53Z 2020-12-10T19:51:53Z 2020-01-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/CompCytogen.v14i1.46852 Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 14, n. 1, p. 61-74, 2020. 1993-0771 http://hdl.handle.net/11449/196653 10.3897/CompCytogen.v14i1.46852 WOS:000518398400001 |
url |
http://dx.doi.org/10.3897/CompCytogen.v14i1.46852 http://hdl.handle.net/11449/196653 |
identifier_str_mv |
Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 14, n. 1, p. 61-74, 2020. 1993-0771 10.3897/CompCytogen.v14i1.46852 WOS:000518398400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
61-74 |
dc.publisher.none.fl_str_mv |
Pensoft Publishers |
publisher.none.fl_str_mv |
Pensoft Publishers |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789244034383872 |