Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents
Autor(a) principal: | |
---|---|
Data de Publicação: | 1998 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/(SICI)1520-6866(1998)18:4<199 http://hdl.handle.net/11449/226123 |
Resumo: | The mutagenicity (clastogenicity) and the carcinogenicity (promoting potential) of cocaine were evaluated, respectively, by the mouse bone marrow micronucleus test (study I) and by the initiated rat liver bioassay (study II). In study I, two administration routes (i.p. and i.v.) and two sampling times (24 and 48 hours) after cocaine treatment were studied. Swiss male mice were treated with cocaine at doses of 0, 18, 37, and 75 mg/kg and 0, 2, 4, and 8 mg/kg by i.p. and i.v. routes, respectively. No significant differences were observed between treated and negative control groups regarding the frequencies of micronuclei and the polichromatic/normochromatic erythrocyte (PCE/NCE) ratios. In study II, the development of putative preneoplastic foci of hepatocytes expressing the enzyme glutathione S-transferase placental form (GST-P+) was utilized as the end-point marker in a 8-week rat liver bioassay. The animals were initiated for carcinogenesis by a single i.p. sub- carcinogenic dose of diethylnitrosamine (DEN). After a 6-week exposure to 5 or 10 mg/kg of cocaine i.v. twice a week there was no enhancement of GST-P+ foci development above the values of the control DEN-only treated animals. Also, cocaine did not induce any toxicity as evidenced by the absence of alterations of rat body and liver weights and of liver biochemical function and morphology. The results suggest that cocaine does not have a mutagenic effect on the mouse bone marrow cells or promoting activity on the rat hepatocarcinogenesis process. |
id |
UNSP_aade91d9461ea620a85441333b4e4686 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/226123 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodentsCocaineMicronucleus testMouse bone marrowRat liver carcinogenesis bioassayThe mutagenicity (clastogenicity) and the carcinogenicity (promoting potential) of cocaine were evaluated, respectively, by the mouse bone marrow micronucleus test (study I) and by the initiated rat liver bioassay (study II). In study I, two administration routes (i.p. and i.v.) and two sampling times (24 and 48 hours) after cocaine treatment were studied. Swiss male mice were treated with cocaine at doses of 0, 18, 37, and 75 mg/kg and 0, 2, 4, and 8 mg/kg by i.p. and i.v. routes, respectively. No significant differences were observed between treated and negative control groups regarding the frequencies of micronuclei and the polichromatic/normochromatic erythrocyte (PCE/NCE) ratios. In study II, the development of putative preneoplastic foci of hepatocytes expressing the enzyme glutathione S-transferase placental form (GST-P+) was utilized as the end-point marker in a 8-week rat liver bioassay. The animals were initiated for carcinogenesis by a single i.p. sub- carcinogenic dose of diethylnitrosamine (DEN). After a 6-week exposure to 5 or 10 mg/kg of cocaine i.v. twice a week there was no enhancement of GST-P+ foci development above the values of the control DEN-only treated animals. Also, cocaine did not induce any toxicity as evidenced by the absence of alterations of rat body and liver weights and of liver biochemical function and morphology. The results suggest that cocaine does not have a mutagenic effect on the mouse bone marrow cells or promoting activity on the rat hepatocarcinogenesis process.Departamento de Patologia Faculdade de Medicina UNESP, Botucatu, SPDepartamento de Nutrifarma USC, Bauru, SPDepartamento de Biologia UEFS, Feira de Santana, BADepartamento de Patologia Faculdade de Medicina Universidade Estadual Paulista, Botucatu, 18618-000, SPDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, SPDepartamento de Patologia Faculdade de Medicina Universidade Estadual Paulista, Botucatu, 18618-000, SPUniversidade Estadual Paulista (UNESP)USCUEFSFavero Salvadori, Daisy Maria [UNESP]Barbisan, Luis Fernando [UNESP]Bazo, Ana Paula [UNESP]De Santana, Efigênia QueirozDenadai, Roberta [UNESP]De Oliveira, Susie Vieira [UNESP]Ribeiro, Lúcia Regina [UNESP]Viana De Camargo, João Lauro [UNESP]2022-04-28T21:25:50Z2022-04-28T21:25:50Z1998-11-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article199-208http://dx.doi.org/10.1002/(SICI)1520-6866(1998)18:4<199Teratogenesis Carcinogenesis and Mutagenesis, v. 18, n. 4, p. 199-208, 1998.0270-3211http://hdl.handle.net/11449/22612310.1002/(SICI)1520-6866(1998)18:4<1992-s2.0-7844235806Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTeratogenesis Carcinogenesis and Mutagenesisinfo:eu-repo/semantics/openAccess2022-04-28T21:25:50Zoai:repositorio.unesp.br:11449/226123Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T21:25:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
title |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
spellingShingle |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents Favero Salvadori, Daisy Maria [UNESP] Cocaine Micronucleus test Mouse bone marrow Rat liver carcinogenesis bioassay |
title_short |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
title_full |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
title_fullStr |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
title_full_unstemmed |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
title_sort |
Cocaine mutagenicity and hepatocarcinogenicity evaluations in rodents |
author |
Favero Salvadori, Daisy Maria [UNESP] |
author_facet |
Favero Salvadori, Daisy Maria [UNESP] Barbisan, Luis Fernando [UNESP] Bazo, Ana Paula [UNESP] De Santana, Efigênia Queiroz Denadai, Roberta [UNESP] De Oliveira, Susie Vieira [UNESP] Ribeiro, Lúcia Regina [UNESP] Viana De Camargo, João Lauro [UNESP] |
author_role |
author |
author2 |
Barbisan, Luis Fernando [UNESP] Bazo, Ana Paula [UNESP] De Santana, Efigênia Queiroz Denadai, Roberta [UNESP] De Oliveira, Susie Vieira [UNESP] Ribeiro, Lúcia Regina [UNESP] Viana De Camargo, João Lauro [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) USC UEFS |
dc.contributor.author.fl_str_mv |
Favero Salvadori, Daisy Maria [UNESP] Barbisan, Luis Fernando [UNESP] Bazo, Ana Paula [UNESP] De Santana, Efigênia Queiroz Denadai, Roberta [UNESP] De Oliveira, Susie Vieira [UNESP] Ribeiro, Lúcia Regina [UNESP] Viana De Camargo, João Lauro [UNESP] |
dc.subject.por.fl_str_mv |
Cocaine Micronucleus test Mouse bone marrow Rat liver carcinogenesis bioassay |
topic |
Cocaine Micronucleus test Mouse bone marrow Rat liver carcinogenesis bioassay |
description |
The mutagenicity (clastogenicity) and the carcinogenicity (promoting potential) of cocaine were evaluated, respectively, by the mouse bone marrow micronucleus test (study I) and by the initiated rat liver bioassay (study II). In study I, two administration routes (i.p. and i.v.) and two sampling times (24 and 48 hours) after cocaine treatment were studied. Swiss male mice were treated with cocaine at doses of 0, 18, 37, and 75 mg/kg and 0, 2, 4, and 8 mg/kg by i.p. and i.v. routes, respectively. No significant differences were observed between treated and negative control groups regarding the frequencies of micronuclei and the polichromatic/normochromatic erythrocyte (PCE/NCE) ratios. In study II, the development of putative preneoplastic foci of hepatocytes expressing the enzyme glutathione S-transferase placental form (GST-P+) was utilized as the end-point marker in a 8-week rat liver bioassay. The animals were initiated for carcinogenesis by a single i.p. sub- carcinogenic dose of diethylnitrosamine (DEN). After a 6-week exposure to 5 or 10 mg/kg of cocaine i.v. twice a week there was no enhancement of GST-P+ foci development above the values of the control DEN-only treated animals. Also, cocaine did not induce any toxicity as evidenced by the absence of alterations of rat body and liver weights and of liver biochemical function and morphology. The results suggest that cocaine does not have a mutagenic effect on the mouse bone marrow cells or promoting activity on the rat hepatocarcinogenesis process. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-11-07 2022-04-28T21:25:50Z 2022-04-28T21:25:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/(SICI)1520-6866(1998)18:4<199 Teratogenesis Carcinogenesis and Mutagenesis, v. 18, n. 4, p. 199-208, 1998. 0270-3211 http://hdl.handle.net/11449/226123 10.1002/(SICI)1520-6866(1998)18:4<199 2-s2.0-7844235806 |
url |
http://dx.doi.org/10.1002/(SICI)1520-6866(1998)18:4<199 http://hdl.handle.net/11449/226123 |
identifier_str_mv |
Teratogenesis Carcinogenesis and Mutagenesis, v. 18, n. 4, p. 199-208, 1998. 0270-3211 10.1002/(SICI)1520-6866(1998)18:4<199 2-s2.0-7844235806 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Teratogenesis Carcinogenesis and Mutagenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
199-208 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046235308818432 |