Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jcb.25035 http://hdl.handle.net/11449/227941 |
Resumo: | Curcumin has therapeutic potential in preventing several types of cancer, including colon, liver, prostate, and breast. The goal of this study was to evaluate the chemopreventive activity of systemically administered curcumin on oral carcinogenesis induced by 4-nitroquinolone-1-oxide (4-NQO). A total of 50 male albino rats, Rattus norvegicus, (Holtzman), were divided into five groups (n = 10 per group). Four of these groups were exposed to 50 ppm 4-NQO in their drinking water ad libitum for 8 or 12 weeks, two groups were treated with curcumin by oral gavage at 30 or 100 mg/kg per day, and one group was treated with corn oil (vehicle) only. The negative control group was euthanized at baseline. Tongues of all animals were removed after euthanasia and used in the subsequent analysis because the tongue is the primary site of carcinogenesis in this model. Descriptive histological analysis and immunohistochemistry for PCNA, Bcl-2, SOCS1 e-3, and STAT3 were performed to assess the oncogenic process. The gene expression of Vimentin, E-cadherin, N-cadherin, or TWIST1 was assessed using RT-qPCR as a representative of epithelial-mesenchymal transition (EMT) events. The administration of curcumin at 100 mg/kg during the 12 weeks markedly decreased the expression of PCNA, Bcl-2, SOCS1 e -3, and STAT3. Curcumin also minimized the cellular atypia under microscopic analysis and diminished the expression of the genes associated with EMT. These findings demonstrate that the systemic administration of curcumin has chemopreventive activity during oral carcinogenesis induced by 4-NQO. J. Cell. Biochem. 116: 787-796, 2015. |
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Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model4-NITROQUINOLINE-1-OXIDECHEMOPREVENTIONCURCUMINRATTONGUECurcumin has therapeutic potential in preventing several types of cancer, including colon, liver, prostate, and breast. The goal of this study was to evaluate the chemopreventive activity of systemically administered curcumin on oral carcinogenesis induced by 4-nitroquinolone-1-oxide (4-NQO). A total of 50 male albino rats, Rattus norvegicus, (Holtzman), were divided into five groups (n = 10 per group). Four of these groups were exposed to 50 ppm 4-NQO in their drinking water ad libitum for 8 or 12 weeks, two groups were treated with curcumin by oral gavage at 30 or 100 mg/kg per day, and one group was treated with corn oil (vehicle) only. The negative control group was euthanized at baseline. Tongues of all animals were removed after euthanasia and used in the subsequent analysis because the tongue is the primary site of carcinogenesis in this model. Descriptive histological analysis and immunohistochemistry for PCNA, Bcl-2, SOCS1 e-3, and STAT3 were performed to assess the oncogenic process. The gene expression of Vimentin, E-cadherin, N-cadherin, or TWIST1 was assessed using RT-qPCR as a representative of epithelial-mesenchymal transition (EMT) events. The administration of curcumin at 100 mg/kg during the 12 weeks markedly decreased the expression of PCNA, Bcl-2, SOCS1 e -3, and STAT3. Curcumin also minimized the cellular atypia under microscopic analysis and diminished the expression of the genes associated with EMT. These findings demonstrate that the systemic administration of curcumin has chemopreventive activity during oral carcinogenesis induced by 4-NQO. J. Cell. Biochem. 116: 787-796, 2015.Department of Diagnosis and Surgery Araraquara School of Dentistry University of São Paulo State UNESPSPDepartment of Biosciences Federal University of São Paulo UNIFESPSPDepartment of Physiology and Pathology Araraquara School of Dentistry University of São Paulo State UNESPSPDepartment of Diagnosis and Surgery Araraquara School of Dentistry University of São Paulo State UNESPSPDepartment of Physiology and Pathology Araraquara School of Dentistry University of São Paulo State UNESPSPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)De Paiva Gonçalves, Vinícius [UNESP]Ortega, Adriana Alicia C. [UNESP]Guimarães, Morgana R. [UNESP]Curylofo, Fabiana Almeida [UNESP]Junior, Carlos Rossa [UNESP]Ribeiro, Daniel ArakiSpolidorio, Luis C. [UNESP]2022-04-29T07:25:54Z2022-04-29T07:25:54Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article787-796http://dx.doi.org/10.1002/jcb.25035Journal of Cellular Biochemistry, v. 116, n. 5, p. 787-796, 2015.1097-46440730-2312http://hdl.handle.net/11449/22794110.1002/jcb.250352-s2.0-84924303224Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Cellular Biochemistryinfo:eu-repo/semantics/openAccess2024-09-27T14:05:16Zoai:repositorio.unesp.br:11449/227941Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
title |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
spellingShingle |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model De Paiva Gonçalves, Vinícius [UNESP] 4-NITROQUINOLINE-1-OXIDE CHEMOPREVENTION CURCUMIN RAT TONGUE |
title_short |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
title_full |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
title_fullStr |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
title_full_unstemmed |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
title_sort |
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model |
author |
De Paiva Gonçalves, Vinícius [UNESP] |
author_facet |
De Paiva Gonçalves, Vinícius [UNESP] Ortega, Adriana Alicia C. [UNESP] Guimarães, Morgana R. [UNESP] Curylofo, Fabiana Almeida [UNESP] Junior, Carlos Rossa [UNESP] Ribeiro, Daniel Araki Spolidorio, Luis C. [UNESP] |
author_role |
author |
author2 |
Ortega, Adriana Alicia C. [UNESP] Guimarães, Morgana R. [UNESP] Curylofo, Fabiana Almeida [UNESP] Junior, Carlos Rossa [UNESP] Ribeiro, Daniel Araki Spolidorio, Luis C. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
De Paiva Gonçalves, Vinícius [UNESP] Ortega, Adriana Alicia C. [UNESP] Guimarães, Morgana R. [UNESP] Curylofo, Fabiana Almeida [UNESP] Junior, Carlos Rossa [UNESP] Ribeiro, Daniel Araki Spolidorio, Luis C. [UNESP] |
dc.subject.por.fl_str_mv |
4-NITROQUINOLINE-1-OXIDE CHEMOPREVENTION CURCUMIN RAT TONGUE |
topic |
4-NITROQUINOLINE-1-OXIDE CHEMOPREVENTION CURCUMIN RAT TONGUE |
description |
Curcumin has therapeutic potential in preventing several types of cancer, including colon, liver, prostate, and breast. The goal of this study was to evaluate the chemopreventive activity of systemically administered curcumin on oral carcinogenesis induced by 4-nitroquinolone-1-oxide (4-NQO). A total of 50 male albino rats, Rattus norvegicus, (Holtzman), were divided into five groups (n = 10 per group). Four of these groups were exposed to 50 ppm 4-NQO in their drinking water ad libitum for 8 or 12 weeks, two groups were treated with curcumin by oral gavage at 30 or 100 mg/kg per day, and one group was treated with corn oil (vehicle) only. The negative control group was euthanized at baseline. Tongues of all animals were removed after euthanasia and used in the subsequent analysis because the tongue is the primary site of carcinogenesis in this model. Descriptive histological analysis and immunohistochemistry for PCNA, Bcl-2, SOCS1 e-3, and STAT3 were performed to assess the oncogenic process. The gene expression of Vimentin, E-cadherin, N-cadherin, or TWIST1 was assessed using RT-qPCR as a representative of epithelial-mesenchymal transition (EMT) events. The administration of curcumin at 100 mg/kg during the 12 weeks markedly decreased the expression of PCNA, Bcl-2, SOCS1 e -3, and STAT3. Curcumin also minimized the cellular atypia under microscopic analysis and diminished the expression of the genes associated with EMT. These findings demonstrate that the systemic administration of curcumin has chemopreventive activity during oral carcinogenesis induced by 4-NQO. J. Cell. Biochem. 116: 787-796, 2015. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2022-04-29T07:25:54Z 2022-04-29T07:25:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jcb.25035 Journal of Cellular Biochemistry, v. 116, n. 5, p. 787-796, 2015. 1097-4644 0730-2312 http://hdl.handle.net/11449/227941 10.1002/jcb.25035 2-s2.0-84924303224 |
url |
http://dx.doi.org/10.1002/jcb.25035 http://hdl.handle.net/11449/227941 |
identifier_str_mv |
Journal of Cellular Biochemistry, v. 116, n. 5, p. 787-796, 2015. 1097-4644 0730-2312 10.1002/jcb.25035 2-s2.0-84924303224 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Cellular Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
787-796 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546450579095552 |