Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs

Detalhes bibliográficos
Autor(a) principal: Ambrósio, DL [UNESP]
Data de Publicação: 2004
Outros Autores: Barbosa, CF [UNESP], Vianna, VF [UNESP], Cicarelli, RMB [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762004000600014
http://hdl.handle.net/11449/8017
Resumo: Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
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spelling Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugstrans-splicingTrypanosoma cruziNFOH-121 (hydroxymethylnitrofurazone)Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)Ambrósio, DL [UNESP]Barbosa, CF [UNESP]Vianna, VF [UNESP]Cicarelli, RMB [UNESP]2014-05-20T13:25:20Z2014-05-20T13:25:20Z2004-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article617-620application/pdfhttp://dx.doi.org/10.1590/S0074-02762004000600014Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 6, p. 617-620, 2004.0074-0276http://hdl.handle.net/11449/801710.1590/S0074-02762004000600014S0074-02762004000600014S0074-02762004000600014.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:07:51Zoai:repositorio.unesp.br:11449/8017Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:13:39.536072Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
title Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
spellingShingle Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
Ambrósio, DL [UNESP]
trans-splicing
Trypanosoma cruzi
NFOH-121 (hydroxymethylnitrofurazone)
title_short Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
title_full Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
title_fullStr Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
title_full_unstemmed Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
title_sort Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
author Ambrósio, DL [UNESP]
author_facet Ambrósio, DL [UNESP]
Barbosa, CF [UNESP]
Vianna, VF [UNESP]
Cicarelli, RMB [UNESP]
author_role author
author2 Barbosa, CF [UNESP]
Vianna, VF [UNESP]
Cicarelli, RMB [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ambrósio, DL [UNESP]
Barbosa, CF [UNESP]
Vianna, VF [UNESP]
Cicarelli, RMB [UNESP]
dc.subject.por.fl_str_mv trans-splicing
Trypanosoma cruzi
NFOH-121 (hydroxymethylnitrofurazone)
topic trans-splicing
Trypanosoma cruzi
NFOH-121 (hydroxymethylnitrofurazone)
description Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
publishDate 2004
dc.date.none.fl_str_mv 2004-10-01
2014-05-20T13:25:20Z
2014-05-20T13:25:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762004000600014
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 6, p. 617-620, 2004.
0074-0276
http://hdl.handle.net/11449/8017
10.1590/S0074-02762004000600014
S0074-02762004000600014
S0074-02762004000600014.pdf
url http://dx.doi.org/10.1590/S0074-02762004000600014
http://hdl.handle.net/11449/8017
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 6, p. 617-620, 2004.
0074-0276
10.1590/S0074-02762004000600014
S0074-02762004000600014
S0074-02762004000600014.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 617-620
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129037431734272

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