Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572008000500003 http://hdl.handle.net/11449/219482 |
Resumo: | From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. |
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Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic ratsBone transplantationDiabetes mellitusWound healingFrom a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.Department of Surgery and Integrate Clinic Dental School of Araçatuba State University of São Paulo, Araçatuba, SPJardim América, Rua João Gonçalves do Nascimento, 1425, 15570-000 Cardoso, SPUniversidade de São Paulo (USP)Jardim AméricaEsteves, Jônatas CaldeiraAranega, Alessandra MarcondesBorrasca, Albanir GabrielFattah, Cristiane Mara Ruiz De SouzaGarcia Jr., Idelmo Rangel2022-04-28T18:55:48Z2022-04-28T18:55:48Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article316-320http://dx.doi.org/10.1590/S1678-77572008000500003Journal of Applied Oral Science, v. 16, n. 5, p. 316-320, 2008.1678-77651678-7757http://hdl.handle.net/11449/21948210.1590/S1678-775720080005000032-s2.0-54349129591Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2022-04-28T18:55:48Zoai:repositorio.unesp.br:11449/219482Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:55:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
spellingShingle |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats Esteves, Jônatas Caldeira Bone transplantation Diabetes mellitus Wound healing |
title_short |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_full |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_fullStr |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_full_unstemmed |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
title_sort |
Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats |
author |
Esteves, Jônatas Caldeira |
author_facet |
Esteves, Jônatas Caldeira Aranega, Alessandra Marcondes Borrasca, Albanir Gabriel Fattah, Cristiane Mara Ruiz De Souza Garcia Jr., Idelmo Rangel |
author_role |
author |
author2 |
Aranega, Alessandra Marcondes Borrasca, Albanir Gabriel Fattah, Cristiane Mara Ruiz De Souza Garcia Jr., Idelmo Rangel |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Jardim América |
dc.contributor.author.fl_str_mv |
Esteves, Jônatas Caldeira Aranega, Alessandra Marcondes Borrasca, Albanir Gabriel Fattah, Cristiane Mara Ruiz De Souza Garcia Jr., Idelmo Rangel |
dc.subject.por.fl_str_mv |
Bone transplantation Diabetes mellitus Wound healing |
topic |
Bone transplantation Diabetes mellitus Wound healing |
description |
From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2022-04-28T18:55:48Z 2022-04-28T18:55:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572008000500003 Journal of Applied Oral Science, v. 16, n. 5, p. 316-320, 2008. 1678-7765 1678-7757 http://hdl.handle.net/11449/219482 10.1590/S1678-77572008000500003 2-s2.0-54349129591 |
url |
http://dx.doi.org/10.1590/S1678-77572008000500003 http://hdl.handle.net/11449/219482 |
identifier_str_mv |
Journal of Applied Oral Science, v. 16, n. 5, p. 316-320, 2008. 1678-7765 1678-7757 10.1590/S1678-77572008000500003 2-s2.0-54349129591 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
316-320 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965085585637376 |