Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1007/s00705-019-04214-z http://hdl.handle.net/11449/188961 |
Resumo: | We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location. |
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Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirusWe describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location.Fundação de Apoio à Pesquisa do Distrito FederalConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Cell Biology Department UnB-University of BrasíliaLaboratory of Insect Virology Department of Biochemistry and Molecular Biology Federal University of Santa MariaPlant Protection Department FCA/UNESP São Paulo State University, Campus of BotucatuApplied Biology Department FCAV/UNESP São Paulo State University, Campus of JaboticabalPlant Protection Department FCA/UNESP São Paulo State University, Campus of BotucatuApplied Biology Department FCAV/UNESP São Paulo State University, Campus of JaboticabalFundação de Apoio à Pesquisa do Distrito Federal: 193.001532/2016CNPq: 407908/2013-7CNPq: 428799/2018-3CAPES: CAPES/PROEX 23038.005848/2018-31UnB-University of BrasíliaFederal University of Santa MariaUniversidade Estadual Paulista (Unesp)Silva, Leonardo A.Ardisson-Araújo, Daniel M. P.Morgado, Fabricio S.Horta, André B. [UNESP]Lemos, Manoel Victor Franco [UNESP]Wilcken, Carlos F. [UNESP]Ribeiro, Bergmann M.2019-10-06T16:25:17Z2019-10-06T16:25:17Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1677-1682http://dx.doi.org/10.1007/s00705-019-04214-zArchives of Virology, v. 164, n. 6, p. 1677-1682, 2019.0304-8608http://hdl.handle.net/11449/18896110.1007/s00705-019-04214-z2-s2.0-850642002767179273060624761Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Virologyinfo:eu-repo/semantics/openAccess2024-06-06T13:03:34Zoai:repositorio.unesp.br:11449/188961Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:40:09.330079Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| title |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| spellingShingle |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus Silva, Leonardo A. |
| title_short |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| title_full |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| title_fullStr |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| title_full_unstemmed |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| title_sort |
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus |
| author |
Silva, Leonardo A. |
| author_facet |
Silva, Leonardo A. Ardisson-Araújo, Daniel M. P. Morgado, Fabricio S. Horta, André B. [UNESP] Lemos, Manoel Victor Franco [UNESP] Wilcken, Carlos F. [UNESP] Ribeiro, Bergmann M. |
| author_role |
author |
| author2 |
Ardisson-Araújo, Daniel M. P. Morgado, Fabricio S. Horta, André B. [UNESP] Lemos, Manoel Victor Franco [UNESP] Wilcken, Carlos F. [UNESP] Ribeiro, Bergmann M. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
UnB-University of Brasília Federal University of Santa Maria Universidade Estadual Paulista (Unesp) |
| dc.contributor.author.fl_str_mv |
Silva, Leonardo A. Ardisson-Araújo, Daniel M. P. Morgado, Fabricio S. Horta, André B. [UNESP] Lemos, Manoel Victor Franco [UNESP] Wilcken, Carlos F. [UNESP] Ribeiro, Bergmann M. |
| description |
We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-10-06T16:25:17Z 2019-10-06T16:25:17Z 2019-06-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00705-019-04214-z Archives of Virology, v. 164, n. 6, p. 1677-1682, 2019. 0304-8608 http://hdl.handle.net/11449/188961 10.1007/s00705-019-04214-z 2-s2.0-85064200276 7179273060624761 |
| url |
http://dx.doi.org/10.1007/s00705-019-04214-z http://hdl.handle.net/11449/188961 |
| identifier_str_mv |
Archives of Virology, v. 164, n. 6, p. 1677-1682, 2019. 0304-8608 10.1007/s00705-019-04214-z 2-s2.0-85064200276 7179273060624761 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Archives of Virology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
1677-1682 |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129541066981376 |