Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2019.102792 http://hdl.handle.net/11449/189650 |
Resumo: | Aiming to investigate the effects of different hormonal protocols on the endometrium morphometry of anestrous mares, 26 animals were assigned to four different treatment groups: (1) EB2.5LAP4: single dose of 2.5 mg of estradiol benzoate (EB); (2) EB5LAP4: 5 mg of EB in 2 consecutive days; (3) EB10LAP4: 10 mg of EB in three consecutive days, considering that all EB-treated groups received a single dose of 1,500 mg of long-acting progesterone (LA P4) after the single/last EB dose; and (4) LAP4: only 1,500 mg of LA P4. Results were also compared with those found in cyclic mares (control group). Endometrial biopsies were collected before and after the hormonal treatments in anestrous mares, and during estrus and at 5 days after ovulation in cyclic mares (D5). Samples were prepared for histological and histomorphometric analysis. Tissue sections were examined to determine luminal epithelium height (LEH), glandular epithelium height (GEH), endometrial thickness (ET), and glandular density (GD). Similar morphometric changes were observed after EB and P4 were administered to groups EB5LAP4 and EB10LAP4. Five days after LA P4 administration (D5), all the assessed variables were similar between all EB-treated groups. In addition, all variables of the EB-treated groups were similar to the control group on D5. Although most of the LAP4 group variables on D5 were similar to the EB-treated groups (except GD), reduced GD and GEH were found when compared with the control group, demonstrating the importance of estradiol priming before P4 on glandular activity and density. |
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Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal ProtocolsEndometriumHistomorphometryHormonesNoncyclic maresAiming to investigate the effects of different hormonal protocols on the endometrium morphometry of anestrous mares, 26 animals were assigned to four different treatment groups: (1) EB2.5LAP4: single dose of 2.5 mg of estradiol benzoate (EB); (2) EB5LAP4: 5 mg of EB in 2 consecutive days; (3) EB10LAP4: 10 mg of EB in three consecutive days, considering that all EB-treated groups received a single dose of 1,500 mg of long-acting progesterone (LA P4) after the single/last EB dose; and (4) LAP4: only 1,500 mg of LA P4. Results were also compared with those found in cyclic mares (control group). Endometrial biopsies were collected before and after the hormonal treatments in anestrous mares, and during estrus and at 5 days after ovulation in cyclic mares (D5). Samples were prepared for histological and histomorphometric analysis. Tissue sections were examined to determine luminal epithelium height (LEH), glandular epithelium height (GEH), endometrial thickness (ET), and glandular density (GD). Similar morphometric changes were observed after EB and P4 were administered to groups EB5LAP4 and EB10LAP4. Five days after LA P4 administration (D5), all the assessed variables were similar between all EB-treated groups. In addition, all variables of the EB-treated groups were similar to the control group on D5. Although most of the LAP4 group variables on D5 were similar to the EB-treated groups (except GD), reduced GD and GEH were found when compared with the control group, demonstrating the importance of estradiol priming before P4 on glandular activity and density.UNIPAC- Faculdade Presidente Antônio CarlosUNESP-Universidade Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal ScienceUNESP-Universidade Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal ScienceUNESP- Universidade Estadual Paulista Campus de Botucatu Department of Anatomy Biosciences InstituteUNESP- Universidade Estadual Paulista Campus de Botucatu Department of Biostatistics Biosciences InstituteUNESP-Universidade Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal ScienceUNESP-Universidade Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal ScienceUNESP- Universidade Estadual Paulista Campus de Botucatu Department of Anatomy Biosciences InstituteUNESP- Universidade Estadual Paulista Campus de Botucatu Department of Biostatistics Biosciences InstituteUNIPAC- Faculdade Presidente Antônio CarlosUniversidade Estadual Paulista (Unesp)Silva, Elisa S.M.Leite, Raíssa O. [UNESP]Maciel, Luciana F.S. [UNESP]Francia, Camila C.D.A. [UNESP]Padovani, Carlos R. [UNESP]Oliveira-Filho, José P. [UNESP]Meira, Cezinande [UNESP]2019-10-06T16:47:30Z2019-10-06T16:47:30Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jevs.2019.102792Journal of Equine Veterinary Science, v. 81.0737-0806http://hdl.handle.net/11449/18965010.1016/j.jevs.2019.1027922-s2.0-8507217109800429054157111990000-0002-2245-800XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-09-09T14:05:40Zoai:repositorio.unesp.br:11449/189650Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
title |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
spellingShingle |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols Silva, Elisa S.M. Endometrium Histomorphometry Hormones Noncyclic mares |
title_short |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
title_full |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
title_fullStr |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
title_full_unstemmed |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
title_sort |
Endometrial Histomorphometry of Anestrous Mares Under the Influence of Different Embryo Transfer Hormonal Protocols |
author |
Silva, Elisa S.M. |
author_facet |
Silva, Elisa S.M. Leite, Raíssa O. [UNESP] Maciel, Luciana F.S. [UNESP] Francia, Camila C.D.A. [UNESP] Padovani, Carlos R. [UNESP] Oliveira-Filho, José P. [UNESP] Meira, Cezinande [UNESP] |
author_role |
author |
author2 |
Leite, Raíssa O. [UNESP] Maciel, Luciana F.S. [UNESP] Francia, Camila C.D.A. [UNESP] Padovani, Carlos R. [UNESP] Oliveira-Filho, José P. [UNESP] Meira, Cezinande [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
UNIPAC- Faculdade Presidente Antônio Carlos Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Elisa S.M. Leite, Raíssa O. [UNESP] Maciel, Luciana F.S. [UNESP] Francia, Camila C.D.A. [UNESP] Padovani, Carlos R. [UNESP] Oliveira-Filho, José P. [UNESP] Meira, Cezinande [UNESP] |
dc.subject.por.fl_str_mv |
Endometrium Histomorphometry Hormones Noncyclic mares |
topic |
Endometrium Histomorphometry Hormones Noncyclic mares |
description |
Aiming to investigate the effects of different hormonal protocols on the endometrium morphometry of anestrous mares, 26 animals were assigned to four different treatment groups: (1) EB2.5LAP4: single dose of 2.5 mg of estradiol benzoate (EB); (2) EB5LAP4: 5 mg of EB in 2 consecutive days; (3) EB10LAP4: 10 mg of EB in three consecutive days, considering that all EB-treated groups received a single dose of 1,500 mg of long-acting progesterone (LA P4) after the single/last EB dose; and (4) LAP4: only 1,500 mg of LA P4. Results were also compared with those found in cyclic mares (control group). Endometrial biopsies were collected before and after the hormonal treatments in anestrous mares, and during estrus and at 5 days after ovulation in cyclic mares (D5). Samples were prepared for histological and histomorphometric analysis. Tissue sections were examined to determine luminal epithelium height (LEH), glandular epithelium height (GEH), endometrial thickness (ET), and glandular density (GD). Similar morphometric changes were observed after EB and P4 were administered to groups EB5LAP4 and EB10LAP4. Five days after LA P4 administration (D5), all the assessed variables were similar between all EB-treated groups. In addition, all variables of the EB-treated groups were similar to the control group on D5. Although most of the LAP4 group variables on D5 were similar to the EB-treated groups (except GD), reduced GD and GEH were found when compared with the control group, demonstrating the importance of estradiol priming before P4 on glandular activity and density. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:47:30Z 2019-10-06T16:47:30Z 2019-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2019.102792 Journal of Equine Veterinary Science, v. 81. 0737-0806 http://hdl.handle.net/11449/189650 10.1016/j.jevs.2019.102792 2-s2.0-85072171098 0042905415711199 0000-0002-2245-800X |
url |
http://dx.doi.org/10.1016/j.jevs.2019.102792 http://hdl.handle.net/11449/189650 |
identifier_str_mv |
Journal of Equine Veterinary Science, v. 81. 0737-0806 10.1016/j.jevs.2019.102792 2-s2.0-85072171098 0042905415711199 0000-0002-2245-800X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Equine Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021338840039424 |