Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration

Detalhes bibliográficos
Autor(a) principal: de Oliveira, Guilherme José Pimentel Lopes
Data de Publicação: 2020
Outros Autores: Aroni, Maurício Andres Tinajero [UNESP], Pinotti, Felipe Eduardo [UNESP], Marcantonio, Elcio [UNESP], Marcantonio, Rosemary Adriana Chiérici [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-019-02943-w
http://hdl.handle.net/11449/200051
Resumo: The aim of this study was to evaluate the osseointegration of implants placed in areas grafted with different osteoconductive bone substitutes irradiated with infrared low-level laser therapy (LLLT). Fifty-six rats were randomly allocated into 4 groups: DBB, bone defects filled with deproteinized bovine bone graft (DBB); HA/TCP, bone defects filled with biphasic ceramic made of hydroxyapatite and β-tricalcium phosphate (HA/TCP); DBB-L, bone defects filled with DBB and treated by LLLT; HA/TCP-L, bone defects filled with HA/TCP and treated by LLLT. Bone defects were performed in the tibia of each animal and filled with the different biomaterials. The grafted areas were treated with LLLT (λ 808 nm, 100 mW, ϕ ∼ 0.60 mm) in 7 sessions with 48 h between the irradiations. After the 60-day period, the implants were placed, and the animals were euthanized after 15 and 45 days. The osseointegration and bone repair in the grafted area were evaluated by biomechanical, microtomographic and histometric analyses, and the expression of some bone biomarkers was evaluated by immunohistochemistry analysis. LLLT induced higher degree of osseointegration, which was associated with the greater expression of BMP2 and OCN. LLLT performed in areas grafted with osteoconductive bone substitutes prior to implant placement improves osseointegration.
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spelling Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegrationBone substitutesLow-level laser therapyOsseointegrationPreclinical studyThe aim of this study was to evaluate the osseointegration of implants placed in areas grafted with different osteoconductive bone substitutes irradiated with infrared low-level laser therapy (LLLT). Fifty-six rats were randomly allocated into 4 groups: DBB, bone defects filled with deproteinized bovine bone graft (DBB); HA/TCP, bone defects filled with biphasic ceramic made of hydroxyapatite and β-tricalcium phosphate (HA/TCP); DBB-L, bone defects filled with DBB and treated by LLLT; HA/TCP-L, bone defects filled with HA/TCP and treated by LLLT. Bone defects were performed in the tibia of each animal and filled with the different biomaterials. The grafted areas were treated with LLLT (λ 808 nm, 100 mW, ϕ ∼ 0.60 mm) in 7 sessions with 48 h between the irradiations. After the 60-day period, the implants were placed, and the animals were euthanized after 15 and 45 days. The osseointegration and bone repair in the grafted area were evaluated by biomechanical, microtomographic and histometric analyses, and the expression of some bone biomarkers was evaluated by immunohistochemistry analysis. LLLT induced higher degree of osseointegration, which was associated with the greater expression of BMP2 and OCN. LLLT performed in areas grafted with osteoconductive bone substitutes prior to implant placement improves osseointegration.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Periodontology Dental School Universidade Federal de Uberlândia (UFU), Pará Av, 1720Department of Diagnosis and Surgery School of Dentistry Campus of Araraquara São Paulo State University (UNESP), Humaitá St. 1680Department of Diagnosis and Surgery School of Dentistry Campus of Araraquara São Paulo State University (UNESP), Humaitá St. 1680FAPESP: 2013/24318-0CNPq: 459984/2014-4Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)de Oliveira, Guilherme José Pimentel LopesAroni, Maurício Andres Tinajero [UNESP]Pinotti, Felipe Eduardo [UNESP]Marcantonio, Elcio [UNESP]Marcantonio, Rosemary Adriana Chiérici [UNESP]2020-12-12T01:56:18Z2020-12-12T01:56:18Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1519-1529http://dx.doi.org/10.1007/s10103-019-02943-wLasers in Medical Science, v. 35, n. 7, p. 1519-1529, 2020.1435-604X0268-8921http://hdl.handle.net/11449/20005110.1007/s10103-019-02943-w2-s2.0-85079180829Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2024-09-26T15:21:56Zoai:repositorio.unesp.br:11449/200051Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:21:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
title Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
spellingShingle Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
de Oliveira, Guilherme José Pimentel Lopes
Bone substitutes
Low-level laser therapy
Osseointegration
Preclinical study
title_short Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
title_full Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
title_fullStr Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
title_full_unstemmed Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
title_sort Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration
author de Oliveira, Guilherme José Pimentel Lopes
author_facet de Oliveira, Guilherme José Pimentel Lopes
Aroni, Maurício Andres Tinajero [UNESP]
Pinotti, Felipe Eduardo [UNESP]
Marcantonio, Elcio [UNESP]
Marcantonio, Rosemary Adriana Chiérici [UNESP]
author_role author
author2 Aroni, Maurício Andres Tinajero [UNESP]
Pinotti, Felipe Eduardo [UNESP]
Marcantonio, Elcio [UNESP]
Marcantonio, Rosemary Adriana Chiérici [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv de Oliveira, Guilherme José Pimentel Lopes
Aroni, Maurício Andres Tinajero [UNESP]
Pinotti, Felipe Eduardo [UNESP]
Marcantonio, Elcio [UNESP]
Marcantonio, Rosemary Adriana Chiérici [UNESP]
dc.subject.por.fl_str_mv Bone substitutes
Low-level laser therapy
Osseointegration
Preclinical study
topic Bone substitutes
Low-level laser therapy
Osseointegration
Preclinical study
description The aim of this study was to evaluate the osseointegration of implants placed in areas grafted with different osteoconductive bone substitutes irradiated with infrared low-level laser therapy (LLLT). Fifty-six rats were randomly allocated into 4 groups: DBB, bone defects filled with deproteinized bovine bone graft (DBB); HA/TCP, bone defects filled with biphasic ceramic made of hydroxyapatite and β-tricalcium phosphate (HA/TCP); DBB-L, bone defects filled with DBB and treated by LLLT; HA/TCP-L, bone defects filled with HA/TCP and treated by LLLT. Bone defects were performed in the tibia of each animal and filled with the different biomaterials. The grafted areas were treated with LLLT (λ 808 nm, 100 mW, ϕ ∼ 0.60 mm) in 7 sessions with 48 h between the irradiations. After the 60-day period, the implants were placed, and the animals were euthanized after 15 and 45 days. The osseointegration and bone repair in the grafted area were evaluated by biomechanical, microtomographic and histometric analyses, and the expression of some bone biomarkers was evaluated by immunohistochemistry analysis. LLLT induced higher degree of osseointegration, which was associated with the greater expression of BMP2 and OCN. LLLT performed in areas grafted with osteoconductive bone substitutes prior to implant placement improves osseointegration.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:56:18Z
2020-12-12T01:56:18Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-019-02943-w
Lasers in Medical Science, v. 35, n. 7, p. 1519-1529, 2020.
1435-604X
0268-8921
http://hdl.handle.net/11449/200051
10.1007/s10103-019-02943-w
2-s2.0-85079180829
url http://dx.doi.org/10.1007/s10103-019-02943-w
http://hdl.handle.net/11449/200051
identifier_str_mv Lasers in Medical Science, v. 35, n. 7, p. 1519-1529, 2020.
1435-604X
0268-8921
10.1007/s10103-019-02943-w
2-s2.0-85079180829
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Medical Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1519-1529
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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