Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms232113571 http://hdl.handle.net/11449/247852 |
Resumo: | A momentary increase in cytoplasmic Ca2+ generates an oscillation responsible for the activation of proteins, such as calmodulin and kinases, which interact with reactive oxygen species (ROS) for the transmission of a stress signal. This study investigated the influence of variations in calcium concentrations on plant defense signaling and photosynthetic acclimatization after mechanical damage. Solanum lycopersicum Micro-Tom was grown with 0, 2 and 4 mM Ca2+, with and without mechanical damage. The expression of stress genes was evaluated, along with levels of antioxidant enzymes, hydrogen peroxide, lipid peroxidation, histochemistry, photosynthesis and dry mass of organs. The ROS production generated by mechanical damage was further enhanced by calcium-free conditions due to the inactivation of the oxygen evolution complex, contributing to an increase in reactive species. The results indicated that ROS affected mechanical damage signaling because calcium-free plants exhibited high levels of H2O2 and enhanced expression of kinase and RBOH1 genes, necessary conditions for an efficient response to stress. We conclude that the plants without calcium supply recognized mechanical damage but did not survive. The highest expression of the RBOH1 gene and the accumulation of H2O2 in these plants signaled cell death. Plants grown in the presence of calcium showed higher expression of SlCaM2 and control of H2O2 concentration, thus overcoming the stress caused by mechanical damage, with photosynthetic acclimatization and without damage to dry mass production. |
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Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damagechlorophyll a fluorescencegas exchangehydrogen peroxideplant stressRBOH1 genesA momentary increase in cytoplasmic Ca2+ generates an oscillation responsible for the activation of proteins, such as calmodulin and kinases, which interact with reactive oxygen species (ROS) for the transmission of a stress signal. This study investigated the influence of variations in calcium concentrations on plant defense signaling and photosynthetic acclimatization after mechanical damage. Solanum lycopersicum Micro-Tom was grown with 0, 2 and 4 mM Ca2+, with and without mechanical damage. The expression of stress genes was evaluated, along with levels of antioxidant enzymes, hydrogen peroxide, lipid peroxidation, histochemistry, photosynthesis and dry mass of organs. The ROS production generated by mechanical damage was further enhanced by calcium-free conditions due to the inactivation of the oxygen evolution complex, contributing to an increase in reactive species. The results indicated that ROS affected mechanical damage signaling because calcium-free plants exhibited high levels of H2O2 and enhanced expression of kinase and RBOH1 genes, necessary conditions for an efficient response to stress. We conclude that the plants without calcium supply recognized mechanical damage but did not survive. The highest expression of the RBOH1 gene and the accumulation of H2O2 in these plants signaled cell death. Plants grown in the presence of calcium showed higher expression of SlCaM2 and control of H2O2 concentration, thus overcoming the stress caused by mechanical damage, with photosynthetic acclimatization and without damage to dry mass production.Biostatistics Plant Biology Parasitology and Zoology Department Institute of Biosciences São Paulo State University (UNESP), Campus Botucatu, Street Prof. Dr. Antônio Celso Wagner Zanin 250, SPPlant Production Department School of Agriculture São Paulo State University (UNESP), Campus Botucatu, Av. Universitária n° 3780, Altos do Paraíso, SPBiostatistics Plant Biology Parasitology and Zoology Department Institute of Biosciences São Paulo State University (UNESP), Campus Botucatu, Street Prof. Dr. Antônio Celso Wagner Zanin 250, SPPlant Production Department School of Agriculture São Paulo State University (UNESP), Campus Botucatu, Av. Universitária n° 3780, Altos do Paraíso, SPUniversidade Estadual Paulista (UNESP)Campos, Felipe Girotto [UNESP]Seixas, Diana Pacheco [UNESP]Barzotto, Gustavo Ribeiro [UNESP]Jorge, Letícia Galhardo [UNESP]Ducatti, Karina Renostro [UNESP]Ferreira, Gisela [UNESP]Rodrigues, Tatiane Maria [UNESP]Silva, Edvaldo Aparecido Amaral da [UNESP]Boaro, Carmen Sílvia Fernandes [UNESP]2023-07-29T13:27:37Z2023-07-29T13:27:37Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms232113571International Journal of Molecular Sciences, v. 23, n. 21, 2022.1422-00671661-6596http://hdl.handle.net/11449/24785210.3390/ijms2321135712-s2.0-85141612210Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2024-04-30T15:58:08Zoai:repositorio.unesp.br:11449/247852Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T15:58:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
title |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
spellingShingle |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage Campos, Felipe Girotto [UNESP] chlorophyll a fluorescence gas exchange hydrogen peroxide plant stress RBOH1 genes |
title_short |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
title_full |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
title_fullStr |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
title_full_unstemmed |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
title_sort |
Roles of Calcium Signaling in Gene Expression and Photosynthetic Acclimatization of Solanum lycopersicum Micro-Tom (MT) after Mechanical Damage |
author |
Campos, Felipe Girotto [UNESP] |
author_facet |
Campos, Felipe Girotto [UNESP] Seixas, Diana Pacheco [UNESP] Barzotto, Gustavo Ribeiro [UNESP] Jorge, Letícia Galhardo [UNESP] Ducatti, Karina Renostro [UNESP] Ferreira, Gisela [UNESP] Rodrigues, Tatiane Maria [UNESP] Silva, Edvaldo Aparecido Amaral da [UNESP] Boaro, Carmen Sílvia Fernandes [UNESP] |
author_role |
author |
author2 |
Seixas, Diana Pacheco [UNESP] Barzotto, Gustavo Ribeiro [UNESP] Jorge, Letícia Galhardo [UNESP] Ducatti, Karina Renostro [UNESP] Ferreira, Gisela [UNESP] Rodrigues, Tatiane Maria [UNESP] Silva, Edvaldo Aparecido Amaral da [UNESP] Boaro, Carmen Sílvia Fernandes [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Campos, Felipe Girotto [UNESP] Seixas, Diana Pacheco [UNESP] Barzotto, Gustavo Ribeiro [UNESP] Jorge, Letícia Galhardo [UNESP] Ducatti, Karina Renostro [UNESP] Ferreira, Gisela [UNESP] Rodrigues, Tatiane Maria [UNESP] Silva, Edvaldo Aparecido Amaral da [UNESP] Boaro, Carmen Sílvia Fernandes [UNESP] |
dc.subject.por.fl_str_mv |
chlorophyll a fluorescence gas exchange hydrogen peroxide plant stress RBOH1 genes |
topic |
chlorophyll a fluorescence gas exchange hydrogen peroxide plant stress RBOH1 genes |
description |
A momentary increase in cytoplasmic Ca2+ generates an oscillation responsible for the activation of proteins, such as calmodulin and kinases, which interact with reactive oxygen species (ROS) for the transmission of a stress signal. This study investigated the influence of variations in calcium concentrations on plant defense signaling and photosynthetic acclimatization after mechanical damage. Solanum lycopersicum Micro-Tom was grown with 0, 2 and 4 mM Ca2+, with and without mechanical damage. The expression of stress genes was evaluated, along with levels of antioxidant enzymes, hydrogen peroxide, lipid peroxidation, histochemistry, photosynthesis and dry mass of organs. The ROS production generated by mechanical damage was further enhanced by calcium-free conditions due to the inactivation of the oxygen evolution complex, contributing to an increase in reactive species. The results indicated that ROS affected mechanical damage signaling because calcium-free plants exhibited high levels of H2O2 and enhanced expression of kinase and RBOH1 genes, necessary conditions for an efficient response to stress. We conclude that the plants without calcium supply recognized mechanical damage but did not survive. The highest expression of the RBOH1 gene and the accumulation of H2O2 in these plants signaled cell death. Plants grown in the presence of calcium showed higher expression of SlCaM2 and control of H2O2 concentration, thus overcoming the stress caused by mechanical damage, with photosynthetic acclimatization and without damage to dry mass production. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T13:27:37Z 2023-07-29T13:27:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms232113571 International Journal of Molecular Sciences, v. 23, n. 21, 2022. 1422-0067 1661-6596 http://hdl.handle.net/11449/247852 10.3390/ijms232113571 2-s2.0-85141612210 |
url |
http://dx.doi.org/10.3390/ijms232113571 http://hdl.handle.net/11449/247852 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 23, n. 21, 2022. 1422-0067 1661-6596 10.3390/ijms232113571 2-s2.0-85141612210 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965344657309696 |