Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients

Detalhes bibliográficos
Autor(a) principal: Kunzmann, Andrew T.
Data de Publicação: 2019
Outros Autores: Proença, Marcela Alcântara [UNESP], Jordao, Haydee Wt, Jiraskova, Katerina, Schneiderova, Michaela, Levy, Miroslav, Liska, Václav, Buchler, Tomas, Vodickova, Ludmila, Vymetalkova, Veronika, Silva, Ana Elizabete [UNESP], Vodicka, Pavel, Hughes, David J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10096-019-03649-1
http://hdl.handle.net/11449/190541
Resumo: There is increasing evidence indicating a role for Fusobacterium nucleatum (F. nucleatum) in colorectal cancer (CRC) development and prognosis. This study evaluated F. nucleatum as a prognostic biomarker, by assessing its association with post-diagnosis survival from CRC. From September 2008 to April 2012 CRC patients (n = 190) were recruited from three hospitals within the Czech Republic. F. nucleatum DNA copies were measured in adjacent non-malignant and colorectal tumor tissues using quantitative real-time PCR. Cox Proportional Hazards (HR) models were applied to evaluate the association between F. nucleatum DNA and overall survival, adjusting for key confounders. Risk prediction modeling was conducted to evaluate the ability to predict survival based on F. nucleatum status. High, compared with low, levels of F. nucleatum in colorectal tumor tissues were associated with poorer overall survival (adjusted HR 1.68, 95% CI 1.02–2.77), which was slightly attenuated after additional adjustment for microsatellite instability status. However, inclusion of F. nucleatum in risk prediction models did not improve the ability to identify patients who died beyond known prognostic factors such as disease pathology staging. Although the increased presence of F. nucleatum was associated with poorer prognosis in CRC patients, this may have limited clinical relevance as a prognostic biomarker.
id UNSP_ac8dc14783b603653aabbac9a9814f31
oai_identifier_str oai:repositorio.unesp.br:11449/190541
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patientsBacterial infectionColorectal cancerColorectal neoplasmDisease survivalFusobacterium nucleatumThere is increasing evidence indicating a role for Fusobacterium nucleatum (F. nucleatum) in colorectal cancer (CRC) development and prognosis. This study evaluated F. nucleatum as a prognostic biomarker, by assessing its association with post-diagnosis survival from CRC. From September 2008 to April 2012 CRC patients (n = 190) were recruited from three hospitals within the Czech Republic. F. nucleatum DNA copies were measured in adjacent non-malignant and colorectal tumor tissues using quantitative real-time PCR. Cox Proportional Hazards (HR) models were applied to evaluate the association between F. nucleatum DNA and overall survival, adjusting for key confounders. Risk prediction modeling was conducted to evaluate the ability to predict survival based on F. nucleatum status. High, compared with low, levels of F. nucleatum in colorectal tumor tissues were associated with poorer overall survival (adjusted HR 1.68, 95% CI 1.02–2.77), which was slightly attenuated after additional adjustment for microsatellite instability status. However, inclusion of F. nucleatum in risk prediction models did not improve the ability to identify patients who died beyond known prognostic factors such as disease pathology staging. Although the increased presence of F. nucleatum was associated with poorer prognosis in CRC patients, this may have limited clinical relevance as a prognostic biomarker.Centre for Public Health Queen’s University BelfastDepartment of Biology São Paulo State University UNESPInstitute of Biology and Medical Genetics First Faculty of Medicine Charles UniversityDepartment of Molecular Biology of Cancer Institute of Experimental Medicine of the Czech Academy of SciencesDepartment of Surgery General University Hospital in PragueDepartment of Surgery First Faculty of Medicine Charles University and Thomayer HospitalBiomedical Centre Faculty of Medicine in Pilsen Charles UniversityDepartment of Oncology First Faculty of Medicine Charles University and Thomayer HospitalCancer Biology and Therapeutics Group School of Biomolecular and Biomedical Science UCD Conway Institute University College DublinDepartment of Biology São Paulo State University UNESPQueen’s University BelfastUniversidade Estadual Paulista (Unesp)Charles UniversityInstitute of Experimental Medicine of the Czech Academy of SciencesGeneral University Hospital in PragueCharles University and Thomayer HospitalUniversity College DublinKunzmann, Andrew T.Proença, Marcela Alcântara [UNESP]Jordao, Haydee WtJiraskova, KaterinaSchneiderova, MichaelaLevy, MiroslavLiska, VáclavBuchler, TomasVodickova, LudmilaVymetalkova, VeronikaSilva, Ana Elizabete [UNESP]Vodicka, PavelHughes, David J.2019-10-06T17:16:30Z2019-10-06T17:16:30Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10096-019-03649-1European Journal of Clinical Microbiology and Infectious Diseases.1435-43730934-9723http://hdl.handle.net/11449/19054110.1007/s10096-019-03649-12-s2.0-85069950591Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Clinical Microbiology and Infectious Diseasesinfo:eu-repo/semantics/openAccess2021-10-23T04:16:33Zoai:repositorio.unesp.br:11449/190541Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:50:25.407266Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
title Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
spellingShingle Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
Kunzmann, Andrew T.
Bacterial infection
Colorectal cancer
Colorectal neoplasm
Disease survival
Fusobacterium nucleatum
title_short Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
title_full Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
title_fullStr Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
title_full_unstemmed Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
title_sort Fusobacterium nucleatum tumor DNA levels are associated with survival in colorectal cancer patients
author Kunzmann, Andrew T.
author_facet Kunzmann, Andrew T.
Proença, Marcela Alcântara [UNESP]
Jordao, Haydee Wt
Jiraskova, Katerina
Schneiderova, Michaela
Levy, Miroslav
Liska, Václav
Buchler, Tomas
Vodickova, Ludmila
Vymetalkova, Veronika
Silva, Ana Elizabete [UNESP]
Vodicka, Pavel
Hughes, David J.
author_role author
author2 Proença, Marcela Alcântara [UNESP]
Jordao, Haydee Wt
Jiraskova, Katerina
Schneiderova, Michaela
Levy, Miroslav
Liska, Václav
Buchler, Tomas
Vodickova, Ludmila
Vymetalkova, Veronika
Silva, Ana Elizabete [UNESP]
Vodicka, Pavel
Hughes, David J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Queen’s University Belfast
Universidade Estadual Paulista (Unesp)
Charles University
Institute of Experimental Medicine of the Czech Academy of Sciences
General University Hospital in Prague
Charles University and Thomayer Hospital
University College Dublin
dc.contributor.author.fl_str_mv Kunzmann, Andrew T.
Proença, Marcela Alcântara [UNESP]
Jordao, Haydee Wt
Jiraskova, Katerina
Schneiderova, Michaela
Levy, Miroslav
Liska, Václav
Buchler, Tomas
Vodickova, Ludmila
Vymetalkova, Veronika
Silva, Ana Elizabete [UNESP]
Vodicka, Pavel
Hughes, David J.
dc.subject.por.fl_str_mv Bacterial infection
Colorectal cancer
Colorectal neoplasm
Disease survival
Fusobacterium nucleatum
topic Bacterial infection
Colorectal cancer
Colorectal neoplasm
Disease survival
Fusobacterium nucleatum
description There is increasing evidence indicating a role for Fusobacterium nucleatum (F. nucleatum) in colorectal cancer (CRC) development and prognosis. This study evaluated F. nucleatum as a prognostic biomarker, by assessing its association with post-diagnosis survival from CRC. From September 2008 to April 2012 CRC patients (n = 190) were recruited from three hospitals within the Czech Republic. F. nucleatum DNA copies were measured in adjacent non-malignant and colorectal tumor tissues using quantitative real-time PCR. Cox Proportional Hazards (HR) models were applied to evaluate the association between F. nucleatum DNA and overall survival, adjusting for key confounders. Risk prediction modeling was conducted to evaluate the ability to predict survival based on F. nucleatum status. High, compared with low, levels of F. nucleatum in colorectal tumor tissues were associated with poorer overall survival (adjusted HR 1.68, 95% CI 1.02–2.77), which was slightly attenuated after additional adjustment for microsatellite instability status. However, inclusion of F. nucleatum in risk prediction models did not improve the ability to identify patients who died beyond known prognostic factors such as disease pathology staging. Although the increased presence of F. nucleatum was associated with poorer prognosis in CRC patients, this may have limited clinical relevance as a prognostic biomarker.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T17:16:30Z
2019-10-06T17:16:30Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10096-019-03649-1
European Journal of Clinical Microbiology and Infectious Diseases.
1435-4373
0934-9723
http://hdl.handle.net/11449/190541
10.1007/s10096-019-03649-1
2-s2.0-85069950591
url http://dx.doi.org/10.1007/s10096-019-03649-1
http://hdl.handle.net/11449/190541
identifier_str_mv European Journal of Clinical Microbiology and Infectious Diseases.
1435-4373
0934-9723
10.1007/s10096-019-03649-1
2-s2.0-85069950591
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal of Clinical Microbiology and Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128282711818240