Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
Autor(a) principal: | |
---|---|
Data de Publicação: | 1997 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0036-46651997000500002 http://hdl.handle.net/11449/65174 |
Resumo: | Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed. |
id |
UNSP_ac9cadafadca2597081875cbe5c90133 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/65174 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting techniqueAscaris suumImmunodiagnosisToxocara canis antigensVisceral larva migransWestern blottingparasite antigenanimalbloodcross reactionfemaleimmunologyToxocara canisvisceral larva migrans syndromeAnimalsAntigens, HelminthBlotting, WesternCross ReactionsFemaleLarva Migrans, VisceralVisceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed.Dept. of Prod. and Animal Health Veterinary Medicine São Paulo State UniversitySerology Section Inst. Adolfo Lutz de São PauloDept. Vet. Prev. Med. Anim. Hlth. Faculty of Veterinary Medicine University of São PauloCurso de Medicina Veterinária UNESP, R. Clovis Pestana 793, Jd. D. Amelia, 16050-680 Araçatuba, SPDept. of Prod. and Animal Health Veterinary Medicine São Paulo State UniversityCurso de Medicina Veterinária UNESP, R. Clovis Pestana 793, Jd. D. Amelia, 16050-680 Araçatuba, SPUniversidade Estadual Paulista (Unesp)Instituto Adolfo Lutz (IAL)Universidade de São Paulo (USP)Nunes, Caris Maroni [UNESP]Tundisi, Regina NardiniGarcia, José Fernando [UNESP]Heinemann, Marcos BrayanOgassawara, SaemiRichtzenhain, Leonardo José2014-05-27T11:18:15Z2014-05-27T11:18:15Z1997-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article253-256application/pdfhttp://dx.doi.org/10.1590/S0036-46651997000500002Revista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997.0036-4665http://hdl.handle.net/11449/6517410.1590/S0036-46651997000500002S0036-466519970005000022-s2.0-0031217069S0036-46651997000500002.pdf18923598712074089991374083045897Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2024-09-04T19:16:18Zoai:repositorio.unesp.br:11449/65174Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
spellingShingle |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique Nunes, Caris Maroni [UNESP] Ascaris suum Immunodiagnosis Toxocara canis antigens Visceral larva migrans Western blotting parasite antigen animal blood cross reaction female immunology Toxocara canis visceral larva migrans syndrome Animals Antigens, Helminth Blotting, Western Cross Reactions Female Larva Migrans, Visceral |
title_short |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_full |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_fullStr |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_full_unstemmed |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
title_sort |
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique |
author |
Nunes, Caris Maroni [UNESP] |
author_facet |
Nunes, Caris Maroni [UNESP] Tundisi, Regina Nardini Garcia, José Fernando [UNESP] Heinemann, Marcos Brayan Ogassawara, Saemi Richtzenhain, Leonardo José |
author_role |
author |
author2 |
Tundisi, Regina Nardini Garcia, José Fernando [UNESP] Heinemann, Marcos Brayan Ogassawara, Saemi Richtzenhain, Leonardo José |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto Adolfo Lutz (IAL) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nunes, Caris Maroni [UNESP] Tundisi, Regina Nardini Garcia, José Fernando [UNESP] Heinemann, Marcos Brayan Ogassawara, Saemi Richtzenhain, Leonardo José |
dc.subject.por.fl_str_mv |
Ascaris suum Immunodiagnosis Toxocara canis antigens Visceral larva migrans Western blotting parasite antigen animal blood cross reaction female immunology Toxocara canis visceral larva migrans syndrome Animals Antigens, Helminth Blotting, Western Cross Reactions Female Larva Migrans, Visceral |
topic |
Ascaris suum Immunodiagnosis Toxocara canis antigens Visceral larva migrans Western blotting parasite antigen animal blood cross reaction female immunology Toxocara canis visceral larva migrans syndrome Animals Antigens, Helminth Blotting, Western Cross Reactions Female Larva Migrans, Visceral |
description |
Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-09-01 2014-05-27T11:18:15Z 2014-05-27T11:18:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0036-46651997000500002 Revista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997. 0036-4665 http://hdl.handle.net/11449/65174 10.1590/S0036-46651997000500002 S0036-46651997000500002 2-s2.0-0031217069 S0036-46651997000500002.pdf 1892359871207408 9991374083045897 |
url |
http://dx.doi.org/10.1590/S0036-46651997000500002 http://hdl.handle.net/11449/65174 |
identifier_str_mv |
Revista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997. 0036-4665 10.1590/S0036-46651997000500002 S0036-46651997000500002 2-s2.0-0031217069 S0036-46651997000500002.pdf 1892359871207408 9991374083045897 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo 1.489 0,669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
253-256 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021420338511872 |