Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique

Detalhes bibliográficos
Autor(a) principal: Nunes, Caris Maroni [UNESP]
Data de Publicação: 1997
Outros Autores: Tundisi, Regina Nardini, Garcia, José Fernando [UNESP], Heinemann, Marcos Brayan, Ogassawara, Saemi, Richtzenhain, Leonardo José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0036-46651997000500002
http://hdl.handle.net/11449/65174
Resumo: Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed.
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spelling Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting techniqueAscaris suumImmunodiagnosisToxocara canis antigensVisceral larva migransWestern blottingparasite antigenanimalbloodcross reactionfemaleimmunologyToxocara canisvisceral larva migrans syndromeAnimalsAntigens, HelminthBlotting, WesternCross ReactionsFemaleLarva Migrans, VisceralVisceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed.Dept. of Prod. and Animal Health Veterinary Medicine São Paulo State UniversitySerology Section Inst. Adolfo Lutz de São PauloDept. Vet. Prev. Med. Anim. Hlth. Faculty of Veterinary Medicine University of São PauloCurso de Medicina Veterinária UNESP, R. Clovis Pestana 793, Jd. D. Amelia, 16050-680 Araçatuba, SPDept. of Prod. and Animal Health Veterinary Medicine São Paulo State UniversityCurso de Medicina Veterinária UNESP, R. Clovis Pestana 793, Jd. D. Amelia, 16050-680 Araçatuba, SPUniversidade Estadual Paulista (Unesp)Instituto Adolfo Lutz (IAL)Universidade de São Paulo (USP)Nunes, Caris Maroni [UNESP]Tundisi, Regina NardiniGarcia, José Fernando [UNESP]Heinemann, Marcos BrayanOgassawara, SaemiRichtzenhain, Leonardo José2014-05-27T11:18:15Z2014-05-27T11:18:15Z1997-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article253-256application/pdfhttp://dx.doi.org/10.1590/S0036-46651997000500002Revista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997.0036-4665http://hdl.handle.net/11449/6517410.1590/S0036-46651997000500002S0036-466519970005000022-s2.0-0031217069S0036-46651997000500002.pdf18923598712074089991374083045897Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2024-09-04T19:16:18Zoai:repositorio.unesp.br:11449/65174Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
title Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
spellingShingle Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
Nunes, Caris Maroni [UNESP]
Ascaris suum
Immunodiagnosis
Toxocara canis antigens
Visceral larva migrans
Western blotting
parasite antigen
animal
blood
cross reaction
female
immunology
Toxocara canis
visceral larva migrans syndrome
Animals
Antigens, Helminth
Blotting, Western
Cross Reactions
Female
Larva Migrans, Visceral
title_short Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
title_full Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
title_fullStr Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
title_full_unstemmed Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
title_sort Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
author Nunes, Caris Maroni [UNESP]
author_facet Nunes, Caris Maroni [UNESP]
Tundisi, Regina Nardini
Garcia, José Fernando [UNESP]
Heinemann, Marcos Brayan
Ogassawara, Saemi
Richtzenhain, Leonardo José
author_role author
author2 Tundisi, Regina Nardini
Garcia, José Fernando [UNESP]
Heinemann, Marcos Brayan
Ogassawara, Saemi
Richtzenhain, Leonardo José
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Adolfo Lutz (IAL)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Nunes, Caris Maroni [UNESP]
Tundisi, Regina Nardini
Garcia, José Fernando [UNESP]
Heinemann, Marcos Brayan
Ogassawara, Saemi
Richtzenhain, Leonardo José
dc.subject.por.fl_str_mv Ascaris suum
Immunodiagnosis
Toxocara canis antigens
Visceral larva migrans
Western blotting
parasite antigen
animal
blood
cross reaction
female
immunology
Toxocara canis
visceral larva migrans syndrome
Animals
Antigens, Helminth
Blotting, Western
Cross Reactions
Female
Larva Migrans, Visceral
topic Ascaris suum
Immunodiagnosis
Toxocara canis antigens
Visceral larva migrans
Western blotting
parasite antigen
animal
blood
cross reaction
female
immunology
Toxocara canis
visceral larva migrans syndrome
Animals
Antigens, Helminth
Blotting, Western
Cross Reactions
Female
Larva Migrans, Visceral
description Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis and A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed.
publishDate 1997
dc.date.none.fl_str_mv 1997-09-01
2014-05-27T11:18:15Z
2014-05-27T11:18:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0036-46651997000500002
Revista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997.
0036-4665
http://hdl.handle.net/11449/65174
10.1590/S0036-46651997000500002
S0036-46651997000500002
2-s2.0-0031217069
S0036-46651997000500002.pdf
1892359871207408
9991374083045897
url http://dx.doi.org/10.1590/S0036-46651997000500002
http://hdl.handle.net/11449/65174
identifier_str_mv Revista do Instituto de Medicina Tropical de Sao Paulo, v. 39, n. 5, p. 253-256, 1997.
0036-4665
10.1590/S0036-46651997000500002
S0036-46651997000500002
2-s2.0-0031217069
S0036-46651997000500002.pdf
1892359871207408
9991374083045897
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
1.489
0,669
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 253-256
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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