Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jim.2019.112664 http://hdl.handle.net/11449/196291 |
Resumo: | Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as plasmonic ELISA (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas. |
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Repositório Institucional da UNESP |
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spelling |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodiesEnzyme-linked immunosorbent assayMetal nanoparticlesCanine LeishmaniasisRecently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as plasmonic ELISA (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Sch Vet Med Cine, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilInfect Dis Res Inst, Seattle, WA USASao Paulo State Univ, Sch Vet Med Cine, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilFAPESP: 2017/11016-6CNPq: 302165/2018-5Elsevier B.V.Universidade Estadual Paulista (Unesp)Infect Dis Res InstSantos Maciel, Marilene Oliveira dos [UNESP]Soares, Matheus Fujimura [UNESP]Costa, Sidnei Ferro [UNESP]Bragato, Jaqueline Poleto [UNESP]Freitas, Jessica Henrique de [UNESP]Venturin, Gabriela Lovizutto [UNESP]Melo, Larissa Martins [UNESP]Rebech, Gabriela Torres [UNESP]Reed, SteveFelix de Lima, Valeria Marcal [UNESP]2020-12-10T19:39:54Z2020-12-10T19:39:54Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6http://dx.doi.org/10.1016/j.jim.2019.112664Journal Of Immunological Methods. Amsterdam: Elsevier, v. 474, 6 p., 2019.0022-1759http://hdl.handle.net/11449/19629110.1016/j.jim.2019.112664WOS:000493970500015Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Immunological Methodsinfo:eu-repo/semantics/openAccess2024-09-04T18:03:57Zoai:repositorio.unesp.br:11449/196291Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
title |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
spellingShingle |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies Santos Maciel, Marilene Oliveira dos [UNESP] Enzyme-linked immunosorbent assay Metal nanoparticles Canine Leishmaniasis |
title_short |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
title_full |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
title_fullStr |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
title_full_unstemmed |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
title_sort |
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies |
author |
Santos Maciel, Marilene Oliveira dos [UNESP] |
author_facet |
Santos Maciel, Marilene Oliveira dos [UNESP] Soares, Matheus Fujimura [UNESP] Costa, Sidnei Ferro [UNESP] Bragato, Jaqueline Poleto [UNESP] Freitas, Jessica Henrique de [UNESP] Venturin, Gabriela Lovizutto [UNESP] Melo, Larissa Martins [UNESP] Rebech, Gabriela Torres [UNESP] Reed, Steve Felix de Lima, Valeria Marcal [UNESP] |
author_role |
author |
author2 |
Soares, Matheus Fujimura [UNESP] Costa, Sidnei Ferro [UNESP] Bragato, Jaqueline Poleto [UNESP] Freitas, Jessica Henrique de [UNESP] Venturin, Gabriela Lovizutto [UNESP] Melo, Larissa Martins [UNESP] Rebech, Gabriela Torres [UNESP] Reed, Steve Felix de Lima, Valeria Marcal [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Infect Dis Res Inst |
dc.contributor.author.fl_str_mv |
Santos Maciel, Marilene Oliveira dos [UNESP] Soares, Matheus Fujimura [UNESP] Costa, Sidnei Ferro [UNESP] Bragato, Jaqueline Poleto [UNESP] Freitas, Jessica Henrique de [UNESP] Venturin, Gabriela Lovizutto [UNESP] Melo, Larissa Martins [UNESP] Rebech, Gabriela Torres [UNESP] Reed, Steve Felix de Lima, Valeria Marcal [UNESP] |
dc.subject.por.fl_str_mv |
Enzyme-linked immunosorbent assay Metal nanoparticles Canine Leishmaniasis |
topic |
Enzyme-linked immunosorbent assay Metal nanoparticles Canine Leishmaniasis |
description |
Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as plasmonic ELISA (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2020-12-10T19:39:54Z 2020-12-10T19:39:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jim.2019.112664 Journal Of Immunological Methods. Amsterdam: Elsevier, v. 474, 6 p., 2019. 0022-1759 http://hdl.handle.net/11449/196291 10.1016/j.jim.2019.112664 WOS:000493970500015 |
url |
http://dx.doi.org/10.1016/j.jim.2019.112664 http://hdl.handle.net/11449/196291 |
identifier_str_mv |
Journal Of Immunological Methods. Amsterdam: Elsevier, v. 474, 6 p., 2019. 0022-1759 10.1016/j.jim.2019.112664 WOS:000493970500015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Immunological Methods |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021388235309056 |