Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies

Detalhes bibliográficos
Autor(a) principal: Santos Maciel, Marilene Oliveira dos [UNESP]
Data de Publicação: 2019
Outros Autores: Soares, Matheus Fujimura [UNESP], Costa, Sidnei Ferro [UNESP], Bragato, Jaqueline Poleto [UNESP], Freitas, Jessica Henrique de [UNESP], Venturin, Gabriela Lovizutto [UNESP], Melo, Larissa Martins [UNESP], Rebech, Gabriela Torres [UNESP], Reed, Steve, Felix de Lima, Valeria Marcal [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jim.2019.112664
http://hdl.handle.net/11449/196291
Resumo: Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as plasmonic ELISA (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas.
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spelling Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodiesEnzyme-linked immunosorbent assayMetal nanoparticlesCanine LeishmaniasisRecently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as plasmonic ELISA (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Sch Vet Med Cine, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilInfect Dis Res Inst, Seattle, WA USASao Paulo State Univ, Sch Vet Med Cine, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilFAPESP: 2017/11016-6CNPq: 302165/2018-5Elsevier B.V.Universidade Estadual Paulista (Unesp)Infect Dis Res InstSantos Maciel, Marilene Oliveira dos [UNESP]Soares, Matheus Fujimura [UNESP]Costa, Sidnei Ferro [UNESP]Bragato, Jaqueline Poleto [UNESP]Freitas, Jessica Henrique de [UNESP]Venturin, Gabriela Lovizutto [UNESP]Melo, Larissa Martins [UNESP]Rebech, Gabriela Torres [UNESP]Reed, SteveFelix de Lima, Valeria Marcal [UNESP]2020-12-10T19:39:54Z2020-12-10T19:39:54Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6http://dx.doi.org/10.1016/j.jim.2019.112664Journal Of Immunological Methods. Amsterdam: Elsevier, v. 474, 6 p., 2019.0022-1759http://hdl.handle.net/11449/19629110.1016/j.jim.2019.112664WOS:000493970500015Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Immunological Methodsinfo:eu-repo/semantics/openAccess2024-09-04T18:03:57Zoai:repositorio.unesp.br:11449/196291Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
title Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
spellingShingle Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
Santos Maciel, Marilene Oliveira dos [UNESP]
Enzyme-linked immunosorbent assay
Metal nanoparticles
Canine Leishmaniasis
title_short Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
title_full Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
title_fullStr Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
title_full_unstemmed Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
title_sort Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
author Santos Maciel, Marilene Oliveira dos [UNESP]
author_facet Santos Maciel, Marilene Oliveira dos [UNESP]
Soares, Matheus Fujimura [UNESP]
Costa, Sidnei Ferro [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Freitas, Jessica Henrique de [UNESP]
Venturin, Gabriela Lovizutto [UNESP]
Melo, Larissa Martins [UNESP]
Rebech, Gabriela Torres [UNESP]
Reed, Steve
Felix de Lima, Valeria Marcal [UNESP]
author_role author
author2 Soares, Matheus Fujimura [UNESP]
Costa, Sidnei Ferro [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Freitas, Jessica Henrique de [UNESP]
Venturin, Gabriela Lovizutto [UNESP]
Melo, Larissa Martins [UNESP]
Rebech, Gabriela Torres [UNESP]
Reed, Steve
Felix de Lima, Valeria Marcal [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Infect Dis Res Inst
dc.contributor.author.fl_str_mv Santos Maciel, Marilene Oliveira dos [UNESP]
Soares, Matheus Fujimura [UNESP]
Costa, Sidnei Ferro [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Freitas, Jessica Henrique de [UNESP]
Venturin, Gabriela Lovizutto [UNESP]
Melo, Larissa Martins [UNESP]
Rebech, Gabriela Torres [UNESP]
Reed, Steve
Felix de Lima, Valeria Marcal [UNESP]
dc.subject.por.fl_str_mv Enzyme-linked immunosorbent assay
Metal nanoparticles
Canine Leishmaniasis
topic Enzyme-linked immunosorbent assay
Metal nanoparticles
Canine Leishmaniasis
description Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as plasmonic ELISA (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-01
2020-12-10T19:39:54Z
2020-12-10T19:39:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jim.2019.112664
Journal Of Immunological Methods. Amsterdam: Elsevier, v. 474, 6 p., 2019.
0022-1759
http://hdl.handle.net/11449/196291
10.1016/j.jim.2019.112664
WOS:000493970500015
url http://dx.doi.org/10.1016/j.jim.2019.112664
http://hdl.handle.net/11449/196291
identifier_str_mv Journal Of Immunological Methods. Amsterdam: Elsevier, v. 474, 6 p., 2019.
0022-1759
10.1016/j.jim.2019.112664
WOS:000493970500015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Immunological Methods
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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