Role of testosterone and androgen receptor in periodontal disease progression in female rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/JPER.19-0099 http://hdl.handle.net/11449/200363 |
Resumo: | Background: Sex hormone therapy has strict recommendations in the treatment of postmenopausal symptoms, in which testosterone (TES) replacement may play a potential role. However, it remains unclear whether TES affects the course of chronic inflammation and alveolar bone loss in females. Herein, we investigated the role of androgen receptor and TES on the inflammatory response and alveolar bone resorption associated with ligature-induced periodontal disease in female rats. Methods: Fifty female Holtzman rats were divided in five groups (n = 10/group): androgen receptor antagonist (flutamide); estrogen receptor antagonist (fulvestrant); TES supplementation; aromatase inhibitor (anastrozole); and TES plus anastrozole. Periodontitis was induced by ligatures around the lower first molars for 2 weeks. Twenty animals (n = 10/group) were used as untreated ligated or non-ligated controls. Bone loss and the number of osteoclasts were measured through radiographic and immunohistochemical analysis, respectively. Inflammatory cytokines, chemokines and bone markers were measured by multiplex immunoassay and ELISA in serum samples and periodontal tissues. Results: The blockage of androgen receptor significantly increased radiographic bone loss and tissue levels of IL-1α (P <0.05), IL-1β (P <0.001) and IL-10 (P <0.01) compared with the periodontitis group. Testosterone supplementation significantly increased EGF levels in tissue samples, whereas when combined with aromatase inhibitor anastrozole significantly increased both EGF and VEGF (P <0.05). None of the treatment conditions significantly impacted the number of osteoclasts compared with the periodontitis group. Conclusions: Androgen receptor activation is an important factor in the regulation of several inflammatory markers, and its blockage significantly increases bone loss. |
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Repositório Institucional da UNESP |
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Role of testosterone and androgen receptor in periodontal disease progression in female ratsalveolar bone lossandrogen receptor antagonistsaromatase inhibitorsestrogen antagonistsinflammationtestosteroneBackground: Sex hormone therapy has strict recommendations in the treatment of postmenopausal symptoms, in which testosterone (TES) replacement may play a potential role. However, it remains unclear whether TES affects the course of chronic inflammation and alveolar bone loss in females. Herein, we investigated the role of androgen receptor and TES on the inflammatory response and alveolar bone resorption associated with ligature-induced periodontal disease in female rats. Methods: Fifty female Holtzman rats were divided in five groups (n = 10/group): androgen receptor antagonist (flutamide); estrogen receptor antagonist (fulvestrant); TES supplementation; aromatase inhibitor (anastrozole); and TES plus anastrozole. Periodontitis was induced by ligatures around the lower first molars for 2 weeks. Twenty animals (n = 10/group) were used as untreated ligated or non-ligated controls. Bone loss and the number of osteoclasts were measured through radiographic and immunohistochemical analysis, respectively. Inflammatory cytokines, chemokines and bone markers were measured by multiplex immunoassay and ELISA in serum samples and periodontal tissues. Results: The blockage of androgen receptor significantly increased radiographic bone loss and tissue levels of IL-1α (P <0.05), IL-1β (P <0.001) and IL-10 (P <0.01) compared with the periodontitis group. Testosterone supplementation significantly increased EGF levels in tissue samples, whereas when combined with aromatase inhibitor anastrozole significantly increased both EGF and VEGF (P <0.05). None of the treatment conditions significantly impacted the number of osteoclasts compared with the periodontitis group. Conclusions: Androgen receptor activation is an important factor in the regulation of several inflammatory markers, and its blockage significantly increases bone loss.Department of Stomatology Universidade Federal do Paraná – UFPRDepartment of Physiology and Pathology School of Dentistry at Araraquara Universidade Estadual Paulista – UNESPDepartment of Applied Oral Sciences Forsyth InstituteDepartment of Physiology and Pathology School of Dentistry at Araraquara Universidade Estadual Paulista – UNESPUniversidade Federal do Paraná (UFPR)Universidade Estadual Paulista (Unesp)Forsyth InstituteSteffens, João PauloValenga, Henrique MeisterSantana, Luis Carlos Leal [UNESP]Albaricci, Maria Carolina da Costa [UNESP]Kantarci, AlpdoganSpolidorio, Luis Carlos [UNESP]2020-12-12T02:04:36Z2020-12-12T02:04:36Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article545-553http://dx.doi.org/10.1002/JPER.19-0099Journal of Periodontology, v. 91, n. 4, p. 545-553, 2020.0022-3492http://hdl.handle.net/11449/20036310.1002/JPER.19-00992-s2.0-85084167295Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Periodontologyinfo:eu-repo/semantics/openAccess2021-10-23T12:31:26Zoai:repositorio.unesp.br:11449/200363Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:02:34.320666Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
title |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
spellingShingle |
Role of testosterone and androgen receptor in periodontal disease progression in female rats Steffens, João Paulo alveolar bone loss androgen receptor antagonists aromatase inhibitors estrogen antagonists inflammation testosterone |
title_short |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
title_full |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
title_fullStr |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
title_full_unstemmed |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
title_sort |
Role of testosterone and androgen receptor in periodontal disease progression in female rats |
author |
Steffens, João Paulo |
author_facet |
Steffens, João Paulo Valenga, Henrique Meister Santana, Luis Carlos Leal [UNESP] Albaricci, Maria Carolina da Costa [UNESP] Kantarci, Alpdogan Spolidorio, Luis Carlos [UNESP] |
author_role |
author |
author2 |
Valenga, Henrique Meister Santana, Luis Carlos Leal [UNESP] Albaricci, Maria Carolina da Costa [UNESP] Kantarci, Alpdogan Spolidorio, Luis Carlos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Universidade Estadual Paulista (Unesp) Forsyth Institute |
dc.contributor.author.fl_str_mv |
Steffens, João Paulo Valenga, Henrique Meister Santana, Luis Carlos Leal [UNESP] Albaricci, Maria Carolina da Costa [UNESP] Kantarci, Alpdogan Spolidorio, Luis Carlos [UNESP] |
dc.subject.por.fl_str_mv |
alveolar bone loss androgen receptor antagonists aromatase inhibitors estrogen antagonists inflammation testosterone |
topic |
alveolar bone loss androgen receptor antagonists aromatase inhibitors estrogen antagonists inflammation testosterone |
description |
Background: Sex hormone therapy has strict recommendations in the treatment of postmenopausal symptoms, in which testosterone (TES) replacement may play a potential role. However, it remains unclear whether TES affects the course of chronic inflammation and alveolar bone loss in females. Herein, we investigated the role of androgen receptor and TES on the inflammatory response and alveolar bone resorption associated with ligature-induced periodontal disease in female rats. Methods: Fifty female Holtzman rats were divided in five groups (n = 10/group): androgen receptor antagonist (flutamide); estrogen receptor antagonist (fulvestrant); TES supplementation; aromatase inhibitor (anastrozole); and TES plus anastrozole. Periodontitis was induced by ligatures around the lower first molars for 2 weeks. Twenty animals (n = 10/group) were used as untreated ligated or non-ligated controls. Bone loss and the number of osteoclasts were measured through radiographic and immunohistochemical analysis, respectively. Inflammatory cytokines, chemokines and bone markers were measured by multiplex immunoassay and ELISA in serum samples and periodontal tissues. Results: The blockage of androgen receptor significantly increased radiographic bone loss and tissue levels of IL-1α (P <0.05), IL-1β (P <0.001) and IL-10 (P <0.01) compared with the periodontitis group. Testosterone supplementation significantly increased EGF levels in tissue samples, whereas when combined with aromatase inhibitor anastrozole significantly increased both EGF and VEGF (P <0.05). None of the treatment conditions significantly impacted the number of osteoclasts compared with the periodontitis group. Conclusions: Androgen receptor activation is an important factor in the regulation of several inflammatory markers, and its blockage significantly increases bone loss. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:04:36Z 2020-12-12T02:04:36Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/JPER.19-0099 Journal of Periodontology, v. 91, n. 4, p. 545-553, 2020. 0022-3492 http://hdl.handle.net/11449/200363 10.1002/JPER.19-0099 2-s2.0-85084167295 |
url |
http://dx.doi.org/10.1002/JPER.19-0099 http://hdl.handle.net/11449/200363 |
identifier_str_mv |
Journal of Periodontology, v. 91, n. 4, p. 545-553, 2020. 0022-3492 10.1002/JPER.19-0099 2-s2.0-85084167295 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Periodontology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
545-553 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129576176451584 |