Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://hdl.handle.net/11449/38625 |
Resumo: | The efficiency of two methods for determining 3-hydroxybutyrate and 3-hydroxyvalerate content in the biopolymer (poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been evaluated. Both methods are based on (a) the hydrolysis of polymers, (b) the esterification with methyl (method A) and isoamilic alcohol (method B) and (c) analysis of the esters by gas chromatography using flame ionization detection (GC-FID). After optimization of the analytical conditions, the main validation parameters (linearity, quantification limit, detection limit, recovery, sensitivity and precision) were determined showing that both analytical procedures can be applied to control the process of poly(3-hydroxybutyrate) production. However, method B gives better analytical results than method A and has the advantage of not using chlorinated solvents. |
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Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradávelValidation of methods for determining the content of 3-hydroxybutyrate and 3-hydroxyvalerate in a biodegradable polymervalidation of methodsbiopolymer analysispolyhydroxyalkanoate compositionThe efficiency of two methods for determining 3-hydroxybutyrate and 3-hydroxyvalerate content in the biopolymer (poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been evaluated. Both methods are based on (a) the hydrolysis of polymers, (b) the esterification with methyl (method A) and isoamilic alcohol (method B) and (c) analysis of the esters by gas chromatography using flame ionization detection (GC-FID). After optimization of the analytical conditions, the main validation parameters (linearity, quantification limit, detection limit, recovery, sensitivity and precision) were determined showing that both analytical procedures can be applied to control the process of poly(3-hydroxybutyrate) production. However, method B gives better analytical results than method A and has the advantage of not using chlorinated solvents.Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilPHB Ind S A, BR-14150000 Serrana, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilSoc Brasileira QuimicaUniversidade Estadual Paulista (Unesp)PHB Ind S ASantos, Ademir dos [UNESP]Polese, Luciana [UNESP]Ribeiro, Clovis Augusto [UNESP]Ernandes, Jose Roberto [UNESP]Dametto, Alessandra Cristina [UNESP]Mangili, Lilian Silveira [UNESP]Kesserlingh, Sonia MariaNonato, Roberto Vianna2014-05-20T15:28:54Z2014-05-20T15:28:54Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1951-1955Quimica Nova. São Paulo: Soc Brasileira Quimica, v. 30, n. 8, p. 1951-1955, 2007.0100-4042http://hdl.handle.net/11449/38625WOS:000252066900029849831089181008249668230218662960000-0002-7984-5908Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporQuímica Nova0.6460,255info:eu-repo/semantics/openAccess2022-03-05T02:25:03Zoai:repositorio.unesp.br:11449/38625Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:17:00.903261Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável Validation of methods for determining the content of 3-hydroxybutyrate and 3-hydroxyvalerate in a biodegradable polymer |
| title |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável |
| spellingShingle |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável Santos, Ademir dos [UNESP] validation of methods biopolymer analysis polyhydroxyalkanoate composition |
| title_short |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável |
| title_full |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável |
| title_fullStr |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável |
| title_full_unstemmed |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável |
| title_sort |
Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável |
| author |
Santos, Ademir dos [UNESP] |
| author_facet |
Santos, Ademir dos [UNESP] Polese, Luciana [UNESP] Ribeiro, Clovis Augusto [UNESP] Ernandes, Jose Roberto [UNESP] Dametto, Alessandra Cristina [UNESP] Mangili, Lilian Silveira [UNESP] Kesserlingh, Sonia Maria Nonato, Roberto Vianna |
| author_role |
author |
| author2 |
Polese, Luciana [UNESP] Ribeiro, Clovis Augusto [UNESP] Ernandes, Jose Roberto [UNESP] Dametto, Alessandra Cristina [UNESP] Mangili, Lilian Silveira [UNESP] Kesserlingh, Sonia Maria Nonato, Roberto Vianna |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) PHB Ind S A |
| dc.contributor.author.fl_str_mv |
Santos, Ademir dos [UNESP] Polese, Luciana [UNESP] Ribeiro, Clovis Augusto [UNESP] Ernandes, Jose Roberto [UNESP] Dametto, Alessandra Cristina [UNESP] Mangili, Lilian Silveira [UNESP] Kesserlingh, Sonia Maria Nonato, Roberto Vianna |
| dc.subject.por.fl_str_mv |
validation of methods biopolymer analysis polyhydroxyalkanoate composition |
| topic |
validation of methods biopolymer analysis polyhydroxyalkanoate composition |
| description |
The efficiency of two methods for determining 3-hydroxybutyrate and 3-hydroxyvalerate content in the biopolymer (poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been evaluated. Both methods are based on (a) the hydrolysis of polymers, (b) the esterification with methyl (method A) and isoamilic alcohol (method B) and (c) analysis of the esters by gas chromatography using flame ionization detection (GC-FID). After optimization of the analytical conditions, the main validation parameters (linearity, quantification limit, detection limit, recovery, sensitivity and precision) were determined showing that both analytical procedures can be applied to control the process of poly(3-hydroxybutyrate) production. However, method B gives better analytical results than method A and has the advantage of not using chlorinated solvents. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-01-01 2014-05-20T15:28:54Z 2014-05-20T15:28:54Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
Quimica Nova. São Paulo: Soc Brasileira Quimica, v. 30, n. 8, p. 1951-1955, 2007. 0100-4042 http://hdl.handle.net/11449/38625 WOS:000252066900029 8498310891810082 4966823021866296 0000-0002-7984-5908 |
| identifier_str_mv |
Quimica Nova. São Paulo: Soc Brasileira Quimica, v. 30, n. 8, p. 1951-1955, 2007. 0100-4042 WOS:000252066900029 8498310891810082 4966823021866296 0000-0002-7984-5908 |
| url |
http://hdl.handle.net/11449/38625 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
Química Nova 0.646 0,255 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
1951-1955 |
| dc.publisher.none.fl_str_mv |
Soc Brasileira Quimica |
| publisher.none.fl_str_mv |
Soc Brasileira Quimica |
| dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
| repository.mail.fl_str_mv |
|
| _version_ |
1808128227417260032 |