Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00705-021-05051-9 http://hdl.handle.net/11449/207642 |
Resumo: | Infection with distinct Zika virus (ZIKV) strains in in vitro and in vivo models has demonstrated that the host’s response to infection is strain-dependent. There has been no analysis of the impact of infection with different ZIKV strains on miRNA expression in human cells. We investigated miRNA expression in PNT1A cells upon infection with an African ZIKV strain (MR766) and a Brazilian ZIKV strain (ZIKVBR) using PCR array. Sixteen miRNAs were modulated in PNT1A cells: six miRNAs were modulated by both strains, while a set of ten miRNAs were modulated exclusively by ZIKVBR infection. In silico analysis showed that nine significant KEGG pathways and eight significant GO terms were predicted to be enriched upon ZIKVBR infection, and these pathways were related to cancer, environmental information processing, metabolism, and extracellular matrix. Differential modulation of miRNA expression suggests that distinct strains of ZIKV can differentially modulate the host response through the action of miRNAs. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strainsInfection with distinct Zika virus (ZIKV) strains in in vitro and in vivo models has demonstrated that the host’s response to infection is strain-dependent. There has been no analysis of the impact of infection with different ZIKV strains on miRNA expression in human cells. We investigated miRNA expression in PNT1A cells upon infection with an African ZIKV strain (MR766) and a Brazilian ZIKV strain (ZIKVBR) using PCR array. Sixteen miRNAs were modulated in PNT1A cells: six miRNAs were modulated by both strains, while a set of ten miRNAs were modulated exclusively by ZIKVBR infection. In silico analysis showed that nine significant KEGG pathways and eight significant GO terms were predicted to be enriched upon ZIKVBR infection, and these pathways were related to cancer, environmental information processing, metabolism, and extracellular matrix. Differential modulation of miRNA expression suggests that distinct strains of ZIKV can differentially modulate the host response through the action of miRNAs.Department of Biology IBILCE Institute of Biosciences Humanities and Exact Sciences UNESP São Paulo State University, Rua Cristóvão Colombo, 2265, Bairro Jardim NazarethDepartment of Biology IBILCE Institute of Biosciences Humanities and Exact Sciences UNESP São Paulo State University, Rua Cristóvão Colombo, 2265, Bairro Jardim NazarethUniversidade Estadual Paulista (Unesp)Machado, Francielly Cristina [UNESP]Bittar, Cíntia [UNESP]Rahal, Paula [UNESP]Calmon, Marilia Freitas [UNESP]2021-06-25T10:58:33Z2021-06-25T10:58:33Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00705-021-05051-9Archives of Virology.1432-87980304-8608http://hdl.handle.net/11449/20764210.1007/s00705-021-05051-92-s2.0-85104670338Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Virologyinfo:eu-repo/semantics/openAccess2021-10-23T17:45:52Zoai:repositorio.unesp.br:11449/207642Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:45:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
title |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
spellingShingle |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains Machado, Francielly Cristina [UNESP] |
title_short |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
title_full |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
title_fullStr |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
title_full_unstemmed |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
title_sort |
Identification of differentially expressed miRNAs in human cells infected with different Zika virus strains |
author |
Machado, Francielly Cristina [UNESP] |
author_facet |
Machado, Francielly Cristina [UNESP] Bittar, Cíntia [UNESP] Rahal, Paula [UNESP] Calmon, Marilia Freitas [UNESP] |
author_role |
author |
author2 |
Bittar, Cíntia [UNESP] Rahal, Paula [UNESP] Calmon, Marilia Freitas [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Machado, Francielly Cristina [UNESP] Bittar, Cíntia [UNESP] Rahal, Paula [UNESP] Calmon, Marilia Freitas [UNESP] |
description |
Infection with distinct Zika virus (ZIKV) strains in in vitro and in vivo models has demonstrated that the host’s response to infection is strain-dependent. There has been no analysis of the impact of infection with different ZIKV strains on miRNA expression in human cells. We investigated miRNA expression in PNT1A cells upon infection with an African ZIKV strain (MR766) and a Brazilian ZIKV strain (ZIKVBR) using PCR array. Sixteen miRNAs were modulated in PNT1A cells: six miRNAs were modulated by both strains, while a set of ten miRNAs were modulated exclusively by ZIKVBR infection. In silico analysis showed that nine significant KEGG pathways and eight significant GO terms were predicted to be enriched upon ZIKVBR infection, and these pathways were related to cancer, environmental information processing, metabolism, and extracellular matrix. Differential modulation of miRNA expression suggests that distinct strains of ZIKV can differentially modulate the host response through the action of miRNAs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:58:33Z 2021-06-25T10:58:33Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00705-021-05051-9 Archives of Virology. 1432-8798 0304-8608 http://hdl.handle.net/11449/207642 10.1007/s00705-021-05051-9 2-s2.0-85104670338 |
url |
http://dx.doi.org/10.1007/s00705-021-05051-9 http://hdl.handle.net/11449/207642 |
identifier_str_mv |
Archives of Virology. 1432-8798 0304-8608 10.1007/s00705-021-05051-9 2-s2.0-85104670338 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Virology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964991388909568 |