Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascianimsci.v37i3.26535 http://hdl.handle.net/11449/158178 |
Resumo: | The bacterial diversity by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was characterized. Three Nellore bovines, cannulated at the rumen, were utilized. Liquid and solid fractions from the rumen content were processed for the extraction of metagenomic DNA and later 16S rDNA amplicons were utilized to construct the WGA library for further clone sequencing. Data were analyzed by MEGA and MOTUR (University of Michigan) softwares. Approximately 97.96% of operation taxonomic units (OTUs) were related to Bacteriodetes phylum and 2.04% of sequences were affiliated to Firmicutes phylum. In the case of Bacteriodetes, the great part of sequences (47.96%) was attributed to Prevotella genus. Bacteroidetes phylum was predominant in rumen content and the Prevotella genus was the most abundant, including diverse species related to this taxon. The bacterial morphological diversity associated to plant fibers was detected by SEM and showed its role in plant biomass deconstruction beyond the detection of microbiological interactions that involved protozoa. |
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Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopyDiversidade bacteriana em rúmen bovino por análise de sequências do 16S rDNA metagenômico e microscopia eletrônica de varredurabacterial communitybacterioidetesNellorePrevotellataxonomic affiliation.comunidade bacterianabacteroidetesNelorePrevotellaafiliação taxonômica.The bacterial diversity by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was characterized. Three Nellore bovines, cannulated at the rumen, were utilized. Liquid and solid fractions from the rumen content were processed for the extraction of metagenomic DNA and later 16S rDNA amplicons were utilized to construct the WGA library for further clone sequencing. Data were analyzed by MEGA and MOTUR (University of Michigan) softwares. Approximately 97.96% of operation taxonomic units (OTUs) were related to Bacteriodetes phylum and 2.04% of sequences were affiliated to Firmicutes phylum. In the case of Bacteriodetes, the great part of sequences (47.96%) was attributed to Prevotella genus. Bacteroidetes phylum was predominant in rumen content and the Prevotella genus was the most abundant, including diverse species related to this taxon. The bacterial morphological diversity associated to plant fibers was detected by SEM and showed its role in plant biomass deconstruction beyond the detection of microbiological interactions that involved protozoa.O estudo foi realizado para caracterizar a diversidade bacteriana por meio do sequenciamento parcial do 16S rDNA total e microscopia eletrônica de varredura (MEV) do microbioma ruminal. Foram utilizados três bovinos da raça Nelore, canulados no rúmen. As frações líquidas e sólidas do conteúdo ruminal foram processadas para extração de DNA metagenômico. Em seguida, amplicons 16S rDNA foram utilizados na construção da biblioteca WGA para posterior sequenciamento dos clones. Os dados foram analisados pelos softwares MEGA e MOTHUR (The University of Michigan). Aproximadamente 97,96% das UTOs foram relacionadas ao filo Bacteroidetes e apenas 2,04% das sequências foram afiliadas ao filo Firmicutes. Para o filo Bacteroidetes, grande parte das sequências (47,96%) foi atribuída ao gênero Prevotella. O filo Bacteroidetes foi predominante no conteúdo ruminal e o gênero Prevotella foi o mais abundante, incluindo diversas espécies relacionadas a esse nível taxonômico. A diversidade morfológica bacteriana associada às fibras vegetais foi detectada por MEV, evidenciando seu papel na desconstrução da biomassa vegetal, além da detecção de interações microbiológicas que envolvem protozoários.Universidade Estadual Paulista Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada à AgropecuáriaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de TecnologiaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada à AgropecuáriaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de TecnologiaEditora da Universidade Estadual de Maringá - EDUEMUniversidade Estadual Paulista (Unesp)Jesus, Raphael Barbetta DeOmori, Wellington PineLemos, Eliana Gertrudes De MacedoSouza, Jackson Antônio Marcondes De2018-11-12T17:28:42Z2018-11-12T17:28:42Z2015-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article251-257application/pdfhttp://dx.doi.org/10.4025/actascianimsci.v37i3.26535Acta Scientiarum. Animal Sciences. Editora da Universidade Estadual de Maringá - EDUEM, v. 37, n. 3, p. 251-257, 2015.1807-8672http://hdl.handle.net/11449/15817810.4025/actascianimsci.v37i3.26535S1807-86722015000300251S1807-86722015000300251.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum. Animal Sciencesinfo:eu-repo/semantics/openAccess2024-06-07T15:32:35Zoai:repositorio.unesp.br:11449/158178Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:11:49.832800Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy Diversidade bacteriana em rúmen bovino por análise de sequências do 16S rDNA metagenômico e microscopia eletrônica de varredura |
title |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
spellingShingle |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy Jesus, Raphael Barbetta De bacterial community bacterioidetes Nellore Prevotella taxonomic affiliation. comunidade bacteriana bacteroidetes Nelore Prevotella afiliação taxonômica. |
title_short |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
title_full |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
title_fullStr |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
title_full_unstemmed |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
title_sort |
Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
author |
Jesus, Raphael Barbetta De |
author_facet |
Jesus, Raphael Barbetta De Omori, Wellington Pine Lemos, Eliana Gertrudes De Macedo Souza, Jackson Antônio Marcondes De |
author_role |
author |
author2 |
Omori, Wellington Pine Lemos, Eliana Gertrudes De Macedo Souza, Jackson Antônio Marcondes De |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Jesus, Raphael Barbetta De Omori, Wellington Pine Lemos, Eliana Gertrudes De Macedo Souza, Jackson Antônio Marcondes De |
dc.subject.por.fl_str_mv |
bacterial community bacterioidetes Nellore Prevotella taxonomic affiliation. comunidade bacteriana bacteroidetes Nelore Prevotella afiliação taxonômica. |
topic |
bacterial community bacterioidetes Nellore Prevotella taxonomic affiliation. comunidade bacteriana bacteroidetes Nelore Prevotella afiliação taxonômica. |
description |
The bacterial diversity by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was characterized. Three Nellore bovines, cannulated at the rumen, were utilized. Liquid and solid fractions from the rumen content were processed for the extraction of metagenomic DNA and later 16S rDNA amplicons were utilized to construct the WGA library for further clone sequencing. Data were analyzed by MEGA and MOTUR (University of Michigan) softwares. Approximately 97.96% of operation taxonomic units (OTUs) were related to Bacteriodetes phylum and 2.04% of sequences were affiliated to Firmicutes phylum. In the case of Bacteriodetes, the great part of sequences (47.96%) was attributed to Prevotella genus. Bacteroidetes phylum was predominant in rumen content and the Prevotella genus was the most abundant, including diverse species related to this taxon. The bacterial morphological diversity associated to plant fibers was detected by SEM and showed its role in plant biomass deconstruction beyond the detection of microbiological interactions that involved protozoa. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 2018-11-12T17:28:42Z 2018-11-12T17:28:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascianimsci.v37i3.26535 Acta Scientiarum. Animal Sciences. Editora da Universidade Estadual de Maringá - EDUEM, v. 37, n. 3, p. 251-257, 2015. 1807-8672 http://hdl.handle.net/11449/158178 10.4025/actascianimsci.v37i3.26535 S1807-86722015000300251 S1807-86722015000300251.pdf |
url |
http://dx.doi.org/10.4025/actascianimsci.v37i3.26535 http://hdl.handle.net/11449/158178 |
identifier_str_mv |
Acta Scientiarum. Animal Sciences. Editora da Universidade Estadual de Maringá - EDUEM, v. 37, n. 3, p. 251-257, 2015. 1807-8672 10.4025/actascianimsci.v37i3.26535 S1807-86722015000300251 S1807-86722015000300251.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum. Animal Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
251-257 application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá - EDUEM |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá - EDUEM |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129403554627584 |