Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actasciagron.v41i1.39332 http://hdl.handle.net/11449/183795 |
Resumo: | ABSTRACT. Triazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics. |
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Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazilblast diseaseCYP51A genesterol demethylation inhibitor (DMI) fungicidesepoxiconazoletebuconazoleABSTRACT. Triazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics.Universidade Federal de LavrasUniversidade Estadual PaulistaEmpresa Brasileira de Pesquisa Agropecuária Centro Nacional de Pesquisa de TrigoUniversidade Estadual PaulistaEditora da Universidade Estadual de Maringá - EDUEMUniversidade Federal de LavrasUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária Centro Nacional de Pesquisa de TrigoDorigan, Adriano FrancisCarvalho, Giselle DePoloni, Nadia MariaNegrisoli, Matheus MerebMaciel, João Leodato NunesCeresini, Paulo Cezar2019-10-03T17:31:40Z2019-10-03T17:31:40Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.4025/actasciagron.v41i1.39332Acta Scientiarum. Agronomy. Editora da Universidade Estadual de Maringá - EDUEM, v. 41, p. -, 2019.1807-8621http://hdl.handle.net/11449/18379510.4025/actasciagron.v41i1.39332S1807-86212019000101003S1807-86212019000101003.pdf26350920583008540000-0003-2381-2792SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum. Agronomyinfo:eu-repo/semantics/openAccess2024-01-13T06:34:44Zoai:repositorio.unesp.br:11449/183795Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:52:52.273592Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
title |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
spellingShingle |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil Dorigan, Adriano Francis blast disease CYP51A gene sterol demethylation inhibitor (DMI) fungicides epoxiconazole tebuconazole |
title_short |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
title_full |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
title_fullStr |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
title_full_unstemmed |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
title_sort |
Resistance to triazole fungicides in Pyricularia species is associated with invasive plants from wheat fields in Brazil |
author |
Dorigan, Adriano Francis |
author_facet |
Dorigan, Adriano Francis Carvalho, Giselle De Poloni, Nadia Maria Negrisoli, Matheus Mereb Maciel, João Leodato Nunes Ceresini, Paulo Cezar |
author_role |
author |
author2 |
Carvalho, Giselle De Poloni, Nadia Maria Negrisoli, Matheus Mereb Maciel, João Leodato Nunes Ceresini, Paulo Cezar |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Lavras Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária Centro Nacional de Pesquisa de Trigo |
dc.contributor.author.fl_str_mv |
Dorigan, Adriano Francis Carvalho, Giselle De Poloni, Nadia Maria Negrisoli, Matheus Mereb Maciel, João Leodato Nunes Ceresini, Paulo Cezar |
dc.subject.por.fl_str_mv |
blast disease CYP51A gene sterol demethylation inhibitor (DMI) fungicides epoxiconazole tebuconazole |
topic |
blast disease CYP51A gene sterol demethylation inhibitor (DMI) fungicides epoxiconazole tebuconazole |
description |
ABSTRACT. Triazole fungicides have not been effective for managing the wheat blast disease in Brazil. A broad analysis across six geographical populations of Pyricularia graminis-tritici in central-southern Brazil indicated a high level of resistance to triazole fungicides. Since P. graminis-tritici is also associated with others poaceous species, here, we analyzed whether triazole-resistant isolates of the blast pathogen could be recovered from other poaceous hosts that are invasive of sprayed wheat fields. In addition to P. graminis-tritici (Pygt), we also evaluated the levels of sensitivity of three other grass-associated blast pathogens, which included P. grisea (Pg), P. pennisetigena (Pp), and P. urashimae (Pu). Resistance to the triazole fungicides tebuconazole and epoxiconazole was assessed phenotypically based on EC50 values and molecularly by analysis of the presence of mutations in the CYP51A gene, which encodes for the target enzyme 14-alpha-demethylase. We detected triazole-resistant Pyricularia spp. (Pg, Pp, Pu and Pygt) that is associated with Avena sativa, Cenchrus echinatus, Chloris distichophylla, Cynodon sp., Digitaria horizontalis, D. sanguinalis, Panicum maximum or Urochloa spp. The major outcome from our study was the evidence that invasive poaceous species from wheat fields could be an important source of triazole resistant fungal inoculum for the initial phases of the wheat blast epidemics. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-03T17:31:40Z 2019-10-03T17:31:40Z 2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actasciagron.v41i1.39332 Acta Scientiarum. Agronomy. Editora da Universidade Estadual de Maringá - EDUEM, v. 41, p. -, 2019. 1807-8621 http://hdl.handle.net/11449/183795 10.4025/actasciagron.v41i1.39332 S1807-86212019000101003 S1807-86212019000101003.pdf 2635092058300854 0000-0003-2381-2792 |
url |
http://dx.doi.org/10.4025/actasciagron.v41i1.39332 http://hdl.handle.net/11449/183795 |
identifier_str_mv |
Acta Scientiarum. Agronomy. Editora da Universidade Estadual de Maringá - EDUEM, v. 41, p. -, 2019. 1807-8621 10.4025/actasciagron.v41i1.39332 S1807-86212019000101003 S1807-86212019000101003.pdf 2635092058300854 0000-0003-2381-2792 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum. Agronomy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá - EDUEM |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá - EDUEM |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129469509009408 |