Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles
Main Author: | |
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Publication Date: | 2016 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.chemosphere.2016.01.076 http://hdl.handle.net/11449/172643 |
Summary: | The use of agrochemicals in agriculture is intense and most of them could be carried out to aquatic environment. Nevertheless, there are only few studies that assess the effects of these xenobiotics on amphibians. Clomazone is an herbicide widely used in rice fields, where amphibian species live. Thus, those species may be threatened by non-target exposure. However, nanoparticles are being developed to be used as a carrier system for the agrochemicals. Such nanoparticles release the herbicide in a modified way, and are considered to be more efficient and less harmful to the environment. The aim of this study was to comparatively evaluate the effect of clomazone in its free form and associated with nanoparticles, in the liver of bullfrog tadpoles (Lithobates catesbeianus) when submitted to acute exposure for 96 h. According to semi-quantitative analysis, there was an increase in the frequency of melanomacrophage centres, in the accumulation of eosinophils and in lipidosis in the liver of experimental groups exposed to clomazone - in its free form and associated with nanoparticles - in comparison with the control group, and the nanotoxicity of chitosan-alginate nanoparticles. The increase of melanomacrophage centres in all exposed groups was significant (P < 0.0001) in comparison to control group. Therefore, the results of this research have shown that exposure to sublethal doses of the herbicide and nanoparticles triggered hepatic responses. Moreover, these results provided important data about the effect of the clomazone herbicide and organic nanoparticles, which act as carriers of agrochemicals, on the bullfrog tadpole liver. |
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Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpolesEcotoxicologyFrogLipidosisLiverMelanomacrophage centresNanotoxicityThe use of agrochemicals in agriculture is intense and most of them could be carried out to aquatic environment. Nevertheless, there are only few studies that assess the effects of these xenobiotics on amphibians. Clomazone is an herbicide widely used in rice fields, where amphibian species live. Thus, those species may be threatened by non-target exposure. However, nanoparticles are being developed to be used as a carrier system for the agrochemicals. Such nanoparticles release the herbicide in a modified way, and are considered to be more efficient and less harmful to the environment. The aim of this study was to comparatively evaluate the effect of clomazone in its free form and associated with nanoparticles, in the liver of bullfrog tadpoles (Lithobates catesbeianus) when submitted to acute exposure for 96 h. According to semi-quantitative analysis, there was an increase in the frequency of melanomacrophage centres, in the accumulation of eosinophils and in lipidosis in the liver of experimental groups exposed to clomazone - in its free form and associated with nanoparticles - in comparison with the control group, and the nanotoxicity of chitosan-alginate nanoparticles. The increase of melanomacrophage centres in all exposed groups was significant (P < 0.0001) in comparison to control group. Therefore, the results of this research have shown that exposure to sublethal doses of the herbicide and nanoparticles triggered hepatic responses. Moreover, these results provided important data about the effect of the clomazone herbicide and organic nanoparticles, which act as carriers of agrochemicals, on the bullfrog tadpole liver.Laboratory of Structural and Functional Biology (LABEF) Universidade Federal de São Carlos (UFSCar), Campus Sorocaba. Rodovia João Leme dos Santos, Km 110 - SP-264Laboratory of Conservation Physiology (LAFISC) Universidade Federal de São Carlos (UFSCar), Campus Sorocaba. Rodovia João Leme dos Santos, Km 110 - SP-264Departament of Environmental Engineering Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Campus Sorocaba. Avenida três de março, n. 511Departament of Environmental Engineering Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Campus Sorocaba. Avenida três de março, n. 511Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Oliveira, Cristiane Ronchi de [UNESP]Fraceto, Leonardo Fernandes [UNESP]Rizzi, Gisele MiglioranzaSalla, Raquel FernandaAbdalla, Fábio CamargoCosta, Monica JonesSilva-Zacarin, Elaine Cristina Mathias2018-12-11T17:01:34Z2018-12-11T17:01:34Z2016-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article304-313application/pdfhttp://dx.doi.org/10.1016/j.chemosphere.2016.01.076Chemosphere, v. 149, p. 304-313.1879-12980045-6535http://hdl.handle.net/11449/17264310.1016/j.chemosphere.2016.01.0762-s2.0-849600986532-s2.0-84960098653.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphere1,435info:eu-repo/semantics/openAccess2023-10-04T06:06:13Zoai:repositorio.unesp.br:11449/172643Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-04T06:06:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
title |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
spellingShingle |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles Oliveira, Cristiane Ronchi de [UNESP] Ecotoxicology Frog Lipidosis Liver Melanomacrophage centres Nanotoxicity |
title_short |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
title_full |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
title_fullStr |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
title_full_unstemmed |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
title_sort |
Hepatic effects of the clomazone herbicide in both its free form and associated with chitosan-alginate nanoparticles in bullfrog tadpoles |
author |
Oliveira, Cristiane Ronchi de [UNESP] |
author_facet |
Oliveira, Cristiane Ronchi de [UNESP] Fraceto, Leonardo Fernandes [UNESP] Rizzi, Gisele Miglioranza Salla, Raquel Fernanda Abdalla, Fábio Camargo Costa, Monica Jones Silva-Zacarin, Elaine Cristina Mathias |
author_role |
author |
author2 |
Fraceto, Leonardo Fernandes [UNESP] Rizzi, Gisele Miglioranza Salla, Raquel Fernanda Abdalla, Fábio Camargo Costa, Monica Jones Silva-Zacarin, Elaine Cristina Mathias |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Cristiane Ronchi de [UNESP] Fraceto, Leonardo Fernandes [UNESP] Rizzi, Gisele Miglioranza Salla, Raquel Fernanda Abdalla, Fábio Camargo Costa, Monica Jones Silva-Zacarin, Elaine Cristina Mathias |
dc.subject.por.fl_str_mv |
Ecotoxicology Frog Lipidosis Liver Melanomacrophage centres Nanotoxicity |
topic |
Ecotoxicology Frog Lipidosis Liver Melanomacrophage centres Nanotoxicity |
description |
The use of agrochemicals in agriculture is intense and most of them could be carried out to aquatic environment. Nevertheless, there are only few studies that assess the effects of these xenobiotics on amphibians. Clomazone is an herbicide widely used in rice fields, where amphibian species live. Thus, those species may be threatened by non-target exposure. However, nanoparticles are being developed to be used as a carrier system for the agrochemicals. Such nanoparticles release the herbicide in a modified way, and are considered to be more efficient and less harmful to the environment. The aim of this study was to comparatively evaluate the effect of clomazone in its free form and associated with nanoparticles, in the liver of bullfrog tadpoles (Lithobates catesbeianus) when submitted to acute exposure for 96 h. According to semi-quantitative analysis, there was an increase in the frequency of melanomacrophage centres, in the accumulation of eosinophils and in lipidosis in the liver of experimental groups exposed to clomazone - in its free form and associated with nanoparticles - in comparison with the control group, and the nanotoxicity of chitosan-alginate nanoparticles. The increase of melanomacrophage centres in all exposed groups was significant (P < 0.0001) in comparison to control group. Therefore, the results of this research have shown that exposure to sublethal doses of the herbicide and nanoparticles triggered hepatic responses. Moreover, these results provided important data about the effect of the clomazone herbicide and organic nanoparticles, which act as carriers of agrochemicals, on the bullfrog tadpole liver. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 2018-12-11T17:01:34Z 2018-12-11T17:01:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2016.01.076 Chemosphere, v. 149, p. 304-313. 1879-1298 0045-6535 http://hdl.handle.net/11449/172643 10.1016/j.chemosphere.2016.01.076 2-s2.0-84960098653 2-s2.0-84960098653.pdf |
url |
http://dx.doi.org/10.1016/j.chemosphere.2016.01.076 http://hdl.handle.net/11449/172643 |
identifier_str_mv |
Chemosphere, v. 149, p. 304-313. 1879-1298 0045-6535 10.1016/j.chemosphere.2016.01.076 2-s2.0-84960098653 2-s2.0-84960098653.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere 1,435 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
304-313 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964431316156416 |