Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.smallrumres.2016.12.030 http://hdl.handle.net/11449/178557 |
Resumo: | The present study aimed at the genetic characterization of five major goat populations of Sri Lanka including four indigenous populations (Jaffna Local – JFL, Kottukachchiya – KOT, Southern – SLS and North Central – SNC) and one stabilized crossbred (German Boer x indigenous goats, also known as “Sri Lankan Boer” – SLB). Genetic diversity was evaluated using 15 microsatellite markers and the mitochondrial DNA D-loop variation. Allelic diversity and observed and expected heterozygosities were moderate, but less than Eurasian and Indian goat breeds. The overall mean estimated inbreeding coefficient (FIS) was 0.069 and significant heterozygote deficiency was detected in JFL (P < 0.001), KOT (P < 0.01) and SLS (P < 0.05), indicating population-specific drift or selection of the loci assessed. Genetic differentiation among populations was low and the phylogenetic clustering pattern was in line with the geographical location of goat populations. Although pair-wise Cavalli-Sforza and Edwards chord distance clustered SLS and SLB separately from the rest of the populations, Bayesian clustering clearly showed lack of discrete genetic structure in Sri Lankan goat populations despite significant morphological and phenotypic differences among them. Mitochondrial DNA D-loop sequences revealed significantly high haplotype diversity with the existence of maternal haplogroups ‘A’ and ‘B’. Analysis of mtDNA sequences indicated maternal origins of Jaffna Local, Kotukachchiya and Sri Lankan South distinct from the other goat populations. |
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Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variationsDNAGenetic diversityGoatHaplogroupMicrosatellitemtDNA D-loopSri LankaThe present study aimed at the genetic characterization of five major goat populations of Sri Lanka including four indigenous populations (Jaffna Local – JFL, Kottukachchiya – KOT, Southern – SLS and North Central – SNC) and one stabilized crossbred (German Boer x indigenous goats, also known as “Sri Lankan Boer” – SLB). Genetic diversity was evaluated using 15 microsatellite markers and the mitochondrial DNA D-loop variation. Allelic diversity and observed and expected heterozygosities were moderate, but less than Eurasian and Indian goat breeds. The overall mean estimated inbreeding coefficient (FIS) was 0.069 and significant heterozygote deficiency was detected in JFL (P < 0.001), KOT (P < 0.01) and SLS (P < 0.05), indicating population-specific drift or selection of the loci assessed. Genetic differentiation among populations was low and the phylogenetic clustering pattern was in line with the geographical location of goat populations. Although pair-wise Cavalli-Sforza and Edwards chord distance clustered SLS and SLB separately from the rest of the populations, Bayesian clustering clearly showed lack of discrete genetic structure in Sri Lankan goat populations despite significant morphological and phenotypic differences among them. Mitochondrial DNA D-loop sequences revealed significantly high haplotype diversity with the existence of maternal haplogroups ‘A’ and ‘B’. Analysis of mtDNA sequences indicated maternal origins of Jaffna Local, Kotukachchiya and Sri Lankan South distinct from the other goat populations.International Atomic Energy AgencyFaculty of Agriculture University of Peradeniya, PeradeniyaUNESP – Univ. Estadual Paulista Faculdade de Medicina Veterinária de AraçatubaJoint FAO/IAEA Division of Nuclear techniques in Food and Agriculture International Atomic Energy AgencyTamil Nadu Veterinary and Animal Sciences UniversityCAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources Institute of Animal Science Chinese Academy of Agricultural Sciences (CAAS)UNESP – Univ. Estadual Paulista Faculdade de Medicina Veterinária de AraçatubaUniversity of PeradeniyaUniversidade Estadual Paulista (Unesp)International Atomic Energy AgencyTamil Nadu Veterinary and Animal Sciences UniversityChinese Academy of Agricultural Sciences (CAAS)Silva, PradeepaDematawewa, C. M.B.Kurukulasuriya, MaheshikaUtsunomiya, Yuri T. [UNESP]Garcia, José Fernando [UNESP]Pichler, RudolfThiruvenkadan, A. K.Ramasamy, SaravananHan, Jian-LinPeriasamy, Kathiravan2018-12-11T17:30:54Z2018-12-11T17:30:54Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51-61application/pdfhttp://dx.doi.org/10.1016/j.smallrumres.2016.12.030Small Ruminant Research, v. 148, p. 51-61.0921-4488http://hdl.handle.net/11449/17855710.1016/j.smallrumres.2016.12.0302-s2.0-850085020952-s2.0-85008502095.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSmall Ruminant Research0,485info:eu-repo/semantics/openAccess2024-09-04T19:15:39Zoai:repositorio.unesp.br:11449/178557Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
title |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
spellingShingle |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations Silva, Pradeepa DNA Genetic diversity Goat Haplogroup Microsatellite mtDNA D-loop Sri Lanka |
title_short |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
title_full |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
title_fullStr |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
title_full_unstemmed |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
title_sort |
Genetic diversity analysis of major Sri Lankan goat populations using microsatellite and mitochondrial DNA D-loop variations |
author |
Silva, Pradeepa |
author_facet |
Silva, Pradeepa Dematawewa, C. M.B. Kurukulasuriya, Maheshika Utsunomiya, Yuri T. [UNESP] Garcia, José Fernando [UNESP] Pichler, Rudolf Thiruvenkadan, A. K. Ramasamy, Saravanan Han, Jian-Lin Periasamy, Kathiravan |
author_role |
author |
author2 |
Dematawewa, C. M.B. Kurukulasuriya, Maheshika Utsunomiya, Yuri T. [UNESP] Garcia, José Fernando [UNESP] Pichler, Rudolf Thiruvenkadan, A. K. Ramasamy, Saravanan Han, Jian-Lin Periasamy, Kathiravan |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Peradeniya Universidade Estadual Paulista (Unesp) International Atomic Energy Agency Tamil Nadu Veterinary and Animal Sciences University Chinese Academy of Agricultural Sciences (CAAS) |
dc.contributor.author.fl_str_mv |
Silva, Pradeepa Dematawewa, C. M.B. Kurukulasuriya, Maheshika Utsunomiya, Yuri T. [UNESP] Garcia, José Fernando [UNESP] Pichler, Rudolf Thiruvenkadan, A. K. Ramasamy, Saravanan Han, Jian-Lin Periasamy, Kathiravan |
dc.subject.por.fl_str_mv |
DNA Genetic diversity Goat Haplogroup Microsatellite mtDNA D-loop Sri Lanka |
topic |
DNA Genetic diversity Goat Haplogroup Microsatellite mtDNA D-loop Sri Lanka |
description |
The present study aimed at the genetic characterization of five major goat populations of Sri Lanka including four indigenous populations (Jaffna Local – JFL, Kottukachchiya – KOT, Southern – SLS and North Central – SNC) and one stabilized crossbred (German Boer x indigenous goats, also known as “Sri Lankan Boer” – SLB). Genetic diversity was evaluated using 15 microsatellite markers and the mitochondrial DNA D-loop variation. Allelic diversity and observed and expected heterozygosities were moderate, but less than Eurasian and Indian goat breeds. The overall mean estimated inbreeding coefficient (FIS) was 0.069 and significant heterozygote deficiency was detected in JFL (P < 0.001), KOT (P < 0.01) and SLS (P < 0.05), indicating population-specific drift or selection of the loci assessed. Genetic differentiation among populations was low and the phylogenetic clustering pattern was in line with the geographical location of goat populations. Although pair-wise Cavalli-Sforza and Edwards chord distance clustered SLS and SLB separately from the rest of the populations, Bayesian clustering clearly showed lack of discrete genetic structure in Sri Lankan goat populations despite significant morphological and phenotypic differences among them. Mitochondrial DNA D-loop sequences revealed significantly high haplotype diversity with the existence of maternal haplogroups ‘A’ and ‘B’. Analysis of mtDNA sequences indicated maternal origins of Jaffna Local, Kotukachchiya and Sri Lankan South distinct from the other goat populations. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:30:54Z 2018-12-11T17:30:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.smallrumres.2016.12.030 Small Ruminant Research, v. 148, p. 51-61. 0921-4488 http://hdl.handle.net/11449/178557 10.1016/j.smallrumres.2016.12.030 2-s2.0-85008502095 2-s2.0-85008502095.pdf |
url |
http://dx.doi.org/10.1016/j.smallrumres.2016.12.030 http://hdl.handle.net/11449/178557 |
identifier_str_mv |
Small Ruminant Research, v. 148, p. 51-61. 0921-4488 10.1016/j.smallrumres.2016.12.030 2-s2.0-85008502095 2-s2.0-85008502095.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Small Ruminant Research 0,485 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
51-61 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021395274399744 |